The characterization of androstenone transport in boar plasma

Bone, Christine; Anderson, Courtney; Lou, Yanping; Squires, E. J.

doi:10.1016/j.jsbmb.2018.09.006

Cited by 5 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This group did not find a specific binding protein for androstenone in the plasma and suggested that androstenone likely binds to albumin or alpha-globulins due to their non-specific binding properties and abundance in porcine plasma. Subsequent work from our laboratory using a novel high-performance liquid chromatography (HPLC) gel filtration technique [41] confirmed that androstenone is bound and transported in the plasma of the boar by albumin. Approximately 80% of androstenone in the plasma is bound to albumin, which dissociates to drive the accumulation of androstenone in the adipose tissue [41].…”

Section: Androstenone Transportmentioning

confidence: 93%

“…Subsequent work from our laboratory using a novel high-performance liquid chromatography (HPLC) gel filtration technique [41] confirmed that androstenone is bound and transported in the plasma of the boar by albumin. Approximately 80% of androstenone in the plasma is bound to albumin, which dissociates to drive the accumulation of androstenone in the adipose tissue [41]. However, the percentage of androstenone displaced from albumin can vary considerably between different animals.…”

Section: Androstenone Transportmentioning

confidence: 93%

See 1 more Smart Citation

Pork Production with Entire Males: Directions for Control of Boar Taint

Squires

Bone

Cameron

2020

Animals

Self Cite

View full text Add to dashboard Cite

Boar taint is caused by the accumulation of androstenone and skatole and other indoles in the fat; this is regulated by the balance between synthesis and degradation of these compounds and can be affected by a number of factors, including environment and management practices, sexual maturity, nutrition, and genetics. Boar taint can be controlled by immunocastration, but this practice has not been accepted in some countries. Genetics offers a long-term solution to the boar taint problem via selective breeding or genome editing. A number of short-term strategies to control boar taint have been proposed, but these can have inconsistent effects and there is too much variability between breeds and individuals to implement a blanket solution for boar taint. Therefore, we propose a precision livestock management approach to developing solutions for controlling taint. This involves determining the differences in metabolic processes and the genetic variations that cause boar taint in specific groups of pigs and using this information to design custom treatments based on the cause of boar taint. Genetic, proteomic or metabolomic profiling can then be used to identify and implement effective solutions for boar taint for specific populations of animals.

show abstract

Section: Androstenone Transportmentioning

confidence: 93%

Section: Androstenone Transportmentioning

confidence: 93%

Pork Production with Entire Males: Directions for Control of Boar Taint

Squires

Bone

Cameron

2020

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some of these compounds display neurosteroid functions [ 215 ], but they mostly have been related to inter-individual communication as pheromones [ 87 , 202 , 268 ] and/or odorous markers [ 205 ]. They are produced by and secreted from skin apocrine glands and other organs such as the brain, adrenals, ovaries and testes [ 83 , 215 ], and have been detected in circulating boar plasma [ 269 ]. The focussing of brain structure/function (and behavior) towards reproduction .…”

Section: The Varied Physiological Functions Of Androgensmentioning

confidence: 99%

The Roles of Androgens in Humans: Biology, Metabolic Regulation and Health

Alemany

2022

IJMS

View full text Add to dashboard Cite

Androgens are an important and diverse group of steroid hormone molecular species. They play varied functional roles, such as the control of metabolic energy fate and partition, the maintenance of skeletal and body protein and integrity and the development of brain capabilities and behavioral setup (including those factors defining maleness). In addition, androgens are the precursors of estrogens, with which they share an extensive control of the reproductive mechanisms (in both sexes). In this review, the types of androgens, their functions and signaling are tabulated and described, including some less-known functions. The close interrelationship between corticosteroids and androgens is also analyzed, centered in the adrenal cortex, together with the main feedback control systems of the hypothalamic–hypophysis–gonads axis, and its modulation by the metabolic environment, sex, age and health. Testosterone (T) is singled out because of its high synthesis rate and turnover, but also because age-related hypogonadism is a key signal for the biologically planned early obsolescence of men, and the delayed onset of a faster rate of functional losses in women after menopause. The close collaboration of T with estradiol (E2) active in the maintenance of body metabolic systems is also presented Their parallel insufficiency has been directly related to the ravages of senescence and the metabolic syndrome constellation of disorders. The clinical use of T to correct hypoandrogenism helps maintain the functionality of core metabolism, limiting excess fat deposition, sarcopenia and cognoscitive frailty (part of these effects are due to the E2 generated from T). The effectiveness of using lipophilic T esters for T replacement treatments is analyzed in depth, and the main problems derived from their application are discussed.

show abstract

“…Recently, a metabolite tentatively identified as androst-3-enol-3-sulfate was detected by liquid chromatography–mass spectrometry from Leydig cell culture and was found to return free androstenone, and not a hydroxylated metabolite, following chemical removal of the sulfate group [ 9 ]. Additionally, we have previously demonstrated that androstenone sulfate has a low binding capacity for porcine albumin, which is the carrier protein responsible for the transport of various steroids in the boar including free androstenone [ 10 , 11 ]. Consequently, androstenone sulfate circulates predominantly unbound in the porcine plasma and is presumably readily available for uptake into peripheral tissues [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar

Bone

Squires

2021

Animals

Self Cite

View full text Add to dashboard Cite

Boars express high testicular levels of sulfotransferase enzymes, and consequently, the boar taint causing compound androstenone predominantly circulates as a steroid sulfate. Androstenone sulfate is suspected to function as a steroid reservoir that can be deconjugated to provide a source of free androstenone for accumulation. Therefore, the purpose of this study was to characterize the uptake and deconjugation of androstenone sulfate in the adipose tissue of the boar. Real-time PCR was used to quantify the expression of steroid sulfatase (STS) and several organic anion transporting polypeptides (OATPs) in the adipose tissue. Additionally, [3H]-androstenone sulfate was incubated with adipocytes or supernatant from homogenized fat to assess steroid uptake and conversion, respectively. A positive correlation existed between OATP-B expression and androstenone sulfate uptake (r = 0.86, p = 0.03), as well as between STS expression and androstenone sulfate conversion (r = 0.76, p < 0.001). Moreover, fat androstenone concentrations were positively correlated (r = 0.85, p < 0.001) with androstenone sulfate conversion and tended to increase with STS expression in early maturing boars. This suggests that androstenone sulfate uptake and deconjugation are mediated by OATP-B and STS, respectively, which may influence the development of boar taint in early maturing animals.

show abstract

The characterization of androstenone transport in boar plasma

Cited by 5 publications

References 15 publications

Pork Production with Entire Males: Directions for Control of Boar Taint

Pork Production with Entire Males: Directions for Control of Boar Taint

The Roles of Androgens in Humans: Biology, Metabolic Regulation and Health

The Uptake and Deconjugation of Androstenone Sulfate in the Adipose Tissue of the Boar

Contact Info

Product

Resources

About