The characterization of an economic and portable LED-based photoacoustic imaging system to facilitate molecular imaging

Hariri, Ali; Lemaster, Jeanne E.; Wang, Junxin; Jeevarathinam, Anantha Krishnan S.; Chao, Daniel L.; Jokerst, Jesse V.

doi:10.1016/j.pacs.2017.11.001

Cited by 151 publications

(147 citation statements)

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“…These initial data were collected with a laser‐based scanner; however, these systems are bulky, delicate, and expensive. More recently, LED‐based photoacoustic imaging system has offered important improvements in size, cost, and stability . Thus, we also evaluated these ulcers with LED‐based photoacoustics.…”

Section: Resultsmentioning

confidence: 99%

“…We used both laser and LED‐based equipment. While the laser‐based system has more power and higher resolution, LED‐based equipment offers significant cost advantages that might aid in clinical translation . The results of LED‐based photoacoustic imaging (Figure ) could easily discriminate between different stages.…”

Section: Discussionmentioning

confidence: 99%

“…We also used a LED‐based photoacoustic imaging system (CYBERDYNE Inc.) . The system is equipped with a 128‐element linear array ultrasound transducer with a central frequency of 10 MHz and a bandwidth of 80.9% fitted with two 690 nm LED arrays.…”

Section: Methodsmentioning

confidence: 99%

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Noninvasive staging of pressure ulcers using photoacoustic imaging

Hariri

Chen

Moore

et al. 2019

Wound Repair Regeneration

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Ulcers including pressure ulcers and diabetic foot ulcers damage the skin and underlying tissue in people with compromised blood circulation. They are classified into four stages of severity and span from mild reddening of the skin to tissue damage and muscle/bone infections. Here, we used photoacoustic imaging as a noninvasive method for detecting early tissue damage that cannot be visually observed while also staging the disease using quantitative image analysis. We used a mouse model of pressure ulcers by implanting subdermal magnets in the dorsal flank and periodically applying an external magnet to the healed implant site. The magnet-induced pressure was applied in cycles, and the extent of ulceration was dictated by the number of cycles. We used both laser-and lightemitting diode (LED)-based photoacoustic imaging tools with 690 nm excitation to evaluate the change in photoacoustic signal and depth of injury. Using laser-based photoacoustic imaging system, we found a 4.4-fold increase in the photoacoustic intensity in stage I vs. baseline (no pressure). We also evaluated the depth of injury using photoacoustics. We measured a photoacoustic ulcer depth of 0.38 AE 0.09 mm, 0.74 AE 0.11 mm, 1.63 AE 0.4 mm, and 2.7 AE 0.31 mm (n = 4) for stages I-IV, respectively. The photoacoustic depth differences between each stage were significant (p < 0.05). We also used an LED-based photoacoustic imaging system to detect early stage (stage I) pressure ulcers and observed a 2.5-fold increase in photoacoustic signal. Importantly, we confirmed the capacity of this technique to detect dysregulated skin even before stage I ulcers have erupted. We also observed significant changes in photoacoustic intensity during healing suggesting that this approach can monitor therapy. These findings were confirmed with histology. These results suggest that this photoacoustic-based approach might have clinical value for monitoring skin diseases including pressure ulcers.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Noninvasive staging of pressure ulcers using photoacoustic imaging

Hariri

Chen

Moore

et al. 2019

Wound Repair Regeneration

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“…Over the past decade, many papers have been published indicating different applications of PAI, for example, breast imaging , ocular imaging , cancer detection and staging and sentinel lymph node (SLN) imaging . Exogenous contrast agents have also been helpful during the development of PAI imaging systems .…”

Section: Introductionmentioning

confidence: 99%

Enhanced contrast acoustic‐resolution photoacoustic microscopy using double‐stage delay‐multiply‐and‐sum beamformer for vasculature imaging

Mozaffarzadeh

Varnosfaderani

Sharma

et al. 2019

Journal of Biophotonics

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In acoustic-resolution photoacoustic microscopy (AR-PAM) systems, the lateral resolution in the focal zone of the ultrasound (US) transducer is determined by the numerical aperture (NA) of the transducer. To have a high lateral resolution, a large NA is used. However, the larger the NA, the smaller the depth of focus [DOF]. As a result, the lateral resolution is deteriorated at depths out of the focal region. The synthetic aperture focusing technique (SAFT) along with a beamformer can be used to improve the resolution outside the focal region. In this work, for image formation in AR-PAM, we propose the double-stage delay-multiply-and-sum (DS_DMAS) algorithm to be combined with SAFT. The proposed method is evaluated experimentally using hair targets and in vivo vasculature imaging. It is shown that DS_DMAS provides a higher resolution and contrast compared to other methods. For the B-mode images obtained using the hair phantom, the proposed method reduces the average noise level for all the depths by about 134%, 57% and 23%, compared to the original low-resolution, SAFT+DAS and SAFT+DMAS methods, respectively. All the results indicate that the proposed method can be an appropriate algorithm for image formation in AR-PAM systems. K E Y W O R D S acoustic-resolution photoacoustic microscopy, contrast enhancement, synthetic aperture focusing technique, vasculature imaging, virtual source Moein Mozaffarzadeh, Mehdi H. H. Varnosfaderan and Arunima Sharma contributed equally to this study.

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“…Detection limits in biological tissues (eg, chicken muscle) ex vivo have also been considered for better approximation of tissue imaging conditions and for exploring the maximum imaging depth achieved using a certain agent concentration . However, excised muscle tissue does not reproduce the complex, dynamic physiological conditions that affect optoacoustic contrast in vivo , such as the hemoglobin distribution.…”

Section: Introductionmentioning

confidence: 99%

Synthetic data framework to estimate the minimum detectable concentration of contrast agents for multispectral optoacoustic imaging of small animals

Yang

Olefir

Tzoumas

et al. 2019

Journal of Biophotonics

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The concentrations of contrast agents for optoacoustic imaging of small animals must usually be optimized through extensive pilot experiments on a case‐by‐case basis. The present work describes a streamlined approach for determining the minimum detectable concentration (MDC) of a contrast agent given experimental conditions and imaging system parameters. The developed Synthetic Data Framework (SDF) allows estimation of MDCs of various contrast agents under different tissue conditions without extensive animal experiments. The SDF combines simulated optoacoustic signals from exogenously administered contrast agents with in vivo experimental signals from background tissue to generate realistic synthetic multispectral optoacoustic images. In this paper, the SDF is validated with in vivo measurements and demonstrates close agreement between SDF synthetic data and experimental data in terms of both image intensity and MDCs. Use of the SDF to estimate MDCs for fluorescent dyes and nanoparticles at different tissue depths and for imaging lesions of different sizes is illustrated.

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The characterization of an economic and portable LED-based photoacoustic imaging system to facilitate molecular imaging

Abstract: Graphical abstract

Cited by 151 publications

References 50 publications

Noninvasive staging of pressure ulcers using photoacoustic imaging

Noninvasive staging of pressure ulcers using photoacoustic imaging

Enhanced contrast acoustic‐resolution photoacoustic microscopy using double‐stage delay‐multiply‐and‐sum beamformer for vasculature imaging

Synthetic data framework to estimate the minimum detectable concentration of contrast agents for multispectral optoacoustic imaging of small animals

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