The Characterization of a Malachite Green Stainable, Glutaraldehyde Extractable Phospholipid in Rabbit Spermatozoa

Teichman, R.; Cummins, James M.; Takei, Gerald H.

doi:10.1095/biolreprod10.5.565

Cited by 40 publications

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“…In fact, they are based on the precipitation of electrondense materials, such as ferric hematoxylin (Kao and Lichtenberger 1987), acid hematein (Fraschini et al 1992), and malachite green (Teichman et al 1974;Boshier et al 1984;Lawton 1989). In mineralized tissues, phospholipids have been detected by malachite green in dentine (Goldberg and Septier 1985), in in vitro bone nodules (Takahashi et al 1991;Nefussi et al 1992), and in epiphyseal cartilage (Bonucci and Silvestrini 1994).…”

Section: Introductionmentioning

confidence: 99%

Influence of specimen preparation on the identification of phospholipids by the phospholipase A2-gold method in mineralizing cartilage and bone

Zini¹,

Sabatelli²,

Silvestrini

et al. 1996

Histochem Cell Biol

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The role of phospholipids in biological mineralization has been hypothesized but not fully elucidated. In order to identify phospholipids at the ultrastructural level in the mineralizing extracellular matrix, rat epiphyseal cartilage and metaphyseal bone have been labeled with the phospholipase A(2) (PLA(2))-gold method. The specificity and the efficiency of phospholipid detection have been evaluated by postembedding labeling of sections from epoxy- or hydrophilic resin-embedded samples, and by preembedding labeling of cryosectioned samples. The efficiency of the labeling was higher in cryosections than in hydrophilic resin-embedded specimens, while lower efficiency was found in epoxy resin-embedded samples. A 2- to 6-fold increase of the labeling density in calcified with respect to uncalcified areas of cartilage and bone has been found, depending on the specimen preparation used. The labeling intensity was significantly higher, at the periphery of the calcifying nodules in the epiphyseal cartilage matrix and in the calcifying osteoid, while the fully calcified bone matrix presented a weak labeling. Matrix vesicles, which are considered a possible source of extracellular phospholipids, appeared labeled in cryosections and in epoxy resin-embedded samples after a preincubation with PLA(2), which also increased the labeling of the intracellular membranes. The localization of phospholipids in the areas of initial mineralization suggests some hypotheses on the possible involvement of these molecules in the mineral phase deposition process.

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Section: Introductionmentioning

confidence: 99%

Influence of specimen preparation on the identification of phospholipids by the phospholipase A2-gold method in mineralizing cartilage and bone

Zini¹,

Sabatelli²,

Silvestrini

et al. 1996

Histochem Cell Biol

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“…Histochemical localization of lipids in various tissues has been done by staining with various reagents such as Sudan black (Berenbaum, 1958), )-phenylenediamine (Boshier et al, 1984), or malachite green (Teichman et al, 1974). With Sudan black B it was possible to stain bound lipids in various tissues and structures, including basement membranes (Berenbaum, 1954).…”

Section: Introductionmentioning

confidence: 99%

“…Malachite green (diaminotriphenylmethane) is a basic dye that has an affinity for lipids and lipid-related substances, including fatty acids, fatty aldehydes, phospholipids, glycolipids, and cholesterol (Teichman et al, 1974). Fixation of tissues in the presence of malachite green followed by post-osmication retains lipid-related materials, which are usually lost after preparation of tissues by rou- tine methods, and also stains them as electron-dense structures.…”

Section: Introductionmentioning

confidence: 99%

“…1978;Teichman et al, 1972Teichman et al, ,1974. Lipids stained by this method are likely to be phospholipids (Boshier et al, 1984;Teichman et al, 1974).…”

Section: Introductionmentioning

confidence: 99%

“…This method was applied at the electron microscopic level for detection of lipids in the rabbit spermatozoa (Teichman et al, 1972(Teichman et al, ,1974, developing rat tooth (Goldberg et al, 1988;Goldberg and Escaig, 1987;Goldberg and Septier, 1985). and in a variety of other tissues (Poucho et al, 1978).…”

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confidence: 99%

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Association of malachite green-positive material with heparan sulfate proteoglycan double tracks in basement membrane of mouse kidney tubules.

Inoué¹

1995

J Histochem Cytochem.

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The presence of lipids in the basement membrane of the mouse kidney tubules was examined by histochemical staining with malachite green. Pieces of mouse kidney cortex were immersed in a fuative containing 3% glutaraldehyde and 0.1% malachite green in 0.067 M sodium cacodylate buffer, pH 6.8, for 18 hr at 4°C. Control tissue was fued in the same way except that no malachite green was added to the fuative. The tissue pieces were ayoprotected, frozen in Freon 22, and subjected to freeze-substitution in dry acetone containing 1% @Or. Thin sections of Epon-embedded specimens were observed by electron microscopy at fmt without uranyl-lead counterstaining. The basement membrane of

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Epididymal Secretion of Carnitine

Cooper

1986

The Epididymis, Sperm Maturation and Fertilisation

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The Characterization of a Malachite Green Stainable, Glutaraldehyde Extractable Phospholipid in Rabbit Spermatozoa

Cited by 40 publications

References 0 publications

Influence of specimen preparation on the identification of phospholipids by the phospholipase A2-gold method in mineralizing cartilage and bone

Influence of specimen preparation on the identification of phospholipids by the phospholipase A2-gold method in mineralizing cartilage and bone

Association of malachite green-positive material with heparan sulfate proteoglycan double tracks in basement membrane of mouse kidney tubules.

Epididymal Secretion of Carnitine

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