The characteristics of multifocal electroretinogram in isolated perfused porcine eye

Abstract

We investigated characteristics of multifocal electroretinograms (mfERG) from in vitro perfused porcine eyes. TTX, NMDA, APB, and PDA were used to identify contributions to the mfERG from inner retinal neurons, ON-pathway, OFF-pathway, and photoreceptors. The cellular contributions of the first-order kernel (K1) in an isolated perfused porcine mfERG came from both inner and outer retina, and were similar to those of in vivo porcine mfERG. In addition, application of isoflurane and propofol caused interference with the mfERG response which resembled inner retinal activities sensitive to TTX + NMDA application. Improved understanding of the cellular origins of the perfused porcine mfERG, in the absence of anesthetic agents, is useful for identifying changes shown in the waveform under anesthesia.

Multifocal electroretinogram for functional evaluation of retinal injury following ischemia–reperfusion in pigs

“…The cellular origin for the response related to mfERG is still under investigation, but both the inner and outer retina then seem to contribute to the P1 wave form [24]. Oxygen consumption in different retinal layers has been investigated [25], but in what way this contributes to the alteration of the P1 is still not understood.…”

Time-Dependent Decline in Multifocal Electroretinogram Requires Faster Recording Procedures in Anesthetized Pigs

“…9 In experimental studies recordings typically last from 3 to 14 minutes. 2,3,6,10–18 The balance between the signal-to-noise ratio and the exhaustion of the examined individual determines the necessary duration of a recording. It is likely that when animals are anesthetized the noise is minimal and shorter recordings would be sufficient.…”