The characteristics of Hep‐2 cell with multiple drug resistance induced by Taxol

Li, Li; Jiang, Alice; Dong, Pin; Wan, Yi; Yu, Zi

doi:10.1016/j.otohns.2007.04.026

Cited by 24 publications

(21 citation statements)

References 18 publications

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“…In a previous study, it was demonstrated that the expression of MDR1/P-gp in human laryngeal cancer tissues was associated with malignant progression and metastasis (9). Furthermore, several studies have reported that MDR1/P-gp may serve a crucial role in the regulation of MDR in laryngeal cancer cells (10,11). Zhigang et al (11) observed that the inhibition of MDR1/P-gp expression served as an effective method to reverse MDR in laryngeal cancer cells.…”

Section: Discussionmentioning

confidence: 98%

“…It is understood that MDR1/P-gp serves a crucial role in regulating intracellular drug concentrations, consequently determining the drug sensitivity of human cells. Recently, MDR1/P-gp has been elucidated to be involved in the regulation of MDR in human laryngeal cancer cells (10,11), with the inhibition of MDR1/P-gp expression having been confirmed as an effective method to reverse MDR in such cells (11). Furthermore, previous data have demonstrated that MDR1/P-gp expression is significantly upregulated in response to hypoxia in laryngeal cancer cells (12).…”

Section: Introductionmentioning

confidence: 99%

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Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells

Zhou

Huang

et al. 2016

Oncology Letters

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Abstract. In a previous study, it was demonstrated that hypoxia upregulated the multidrug resistance (MDR) of laryngeal cancer cells to chemotherapeutic drugs, with multidrug resistance 1 (MDR1)/P-glycoprotein (P-gp) expression also being upregulated. The present study aimed to investigate the role and mechanism of MDR1/P-gp on hypoxia-induced MDR in human laryngeal carcinoma cells. The sensitivity of laryngeal cancer cells to multiple drugs and cisplatin-induced apoptosis was determined by CCK-8 assay and Annexin-V/propidium iodide staining analysis, respectively. The accumulation of rhodamine 123 (Rh123) in the cells served as an estimate of drug accumulation and was evaluated by flow cytometry (FCM). MDR1/P-gp expression was inhibited using interference RNA, and the expression of the MDR1 gene was analyzed using reverse transcription-quantitative polymerase chain reaction and western blotting. As a result, the sensitivity to multiple chemotherapeutic agents and the apoptosis rate of the hypoxic laryngeal carcinoma cells increased following a decrease in MDR1/P-gp expression (P<0.05). Additionally, FCM analysis of fluorescence intensity indicated that the downregulated expression of MDR1/P-gp markedly increased intracellular Rh123 accumulation (P<0.05). Such results suggest that MDR1/P-gp serves an important role in regulating hypoxia-induced MDR in human laryngeal carcinoma cells through a decrease in intracellular drug accumulation.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells

Zhou

Huang

et al. 2016

Oncology Letters

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“…The CNE, Hep-2 and FaDu cell lines from human nasopharyngeal, laryngeal and hypopharyngeal cancers, were obtained from the American Type Culture Collection (ATCC). A taxol-resistant cell line (Hep-2T) was developed by continuous exposure of Hep-2 cells to stepwise escalating concentrations of taxol for 12 months (24). All of the cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin at 37˚C in a humidified atmosphere composed of 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Expression and function of ABCG2 in head and neck squamous cell carcinoma and cell lines

Shen

Dong

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. Overexpression of breast cancer resistance protein, the ATP-binding cassette, subfamily G, member2 (BCRP/ABCG2), confers multidrug resistance to tumor cells and often limits the efficacy of chemotherapy. The aim of this study was to investigate the expression and functional activity of ABCG2 in head and neck squamous cell carcinoma (HNSCC) and corresponding cell lines. Immunohistochemistry was performed to investigate the presence of the ABCG2 transporter in HNSCC tissues. Expression of ABCG2 in the Hep-2, Hep-2T, CNE and FaDu cell lines was analyzed by real-time quantitative reverse transcription-polymerase chain reaction and Western blotting at the levels of messenger RNA (mRNA) and protein, respectively. The drug sensitivity of the above four cell lines to mitoxantrone was detected using MTT, and the drug accumulation of mitoxantrone was analyzed by flow cytometry. Positive expression of ABCG2 was detected in 52.04% of the laryngeal cancer samples from 98 patients, in 65% of the 40 hypopharyngeal cancer samples and in 58.82% of the 34 nasopharyngeal cancer samples. The level of expression was found to be correlated with tumor TNM stage (P<0.05) and lymph node metastasis (P<0.01). All four HNSCC cell lines expressed ABCG2 at the mRNA and protein levels. The levels of ABCG2 expression in the four cell lines were significantly correlated with the function and sensitivity to mitoxantrone. The addition of fumitremorgin C at a concentration of 5 µM to mitoxantrone treatment caused a varied increase in mitoxantrone accumulation of 1.09-fold, 1.33-fold (P<0.01), 1.4-fold (P<0.01) and 1-fold in the Hep-2, Hep-2T, CNE and FaDu cells, respectively. Expression of ABCG2 varied among the different types of carcinoma tissues and each HNSCC cell line, and it induced multidrug resistance and separation of cancer stem cells attributing to its efflux pump function. Thus, ABCG2 expression may be an unfavorable prognostic factor for HNSCC. Due to the negligible expression and function of ABCG2, we suggest that the FaDu cell line is suitable to be a negative control in studies involving HNSCC. Taken together, ABCG2 is a promising universal biomarker of cancer stem cells and a target gene for HNSCC chemotherapy.

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“…The laryngeal carcinoma cells Hep-2 was gained from the American Type Culture Collection (ATCC) and multidrug-resistant cell variant Hep-2T had been prepared and characterized in our previous work (Li et al, 2007). All cell lines were maintained as monolayers in Dulbecco's modification of Eagle's medium (DMEM; Gibco Corporation, USA), supplemented with 10% fetal bovine serum (Hyclone, USA) and antibiotics (100 IU/ ml penicillin and 100 IU/ml streptomycin) at 37°C in a humidified atmosphere containing 95% air and 5% CO 2 .…”

Section: Cell Line and Culturementioning

confidence: 99%

Hypoxia Induced Multidrug Resistance of Laryngeal Cancer Cells via Hypoxia-inducible Factor-1α

Li¹,

Dong²,

Wang³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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The characteristics of Hep‐2 cell with multiple drug resistance induced by Taxol

Abstract: These findings may provide theoretical support for the reverse of MDR.

Cited by 24 publications

References 18 publications

Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells

Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells

Expression and function of ABCG2 in head and neck squamous cell carcinoma and cell lines

Hypoxia Induced Multidrug Resistance of Laryngeal Cancer Cells via Hypoxia-inducible Factor-1α

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