The Characteristics of Cultured Mucosal Cell Sheet as a Material for Grafting; Comparison with Cultured Epidermal Cell Sheet

Hata, Ken‐ichiro; Kagami, Hideaki; Ueda, Minoru; Torii, Shuhei; Matsuyama, Mutsushi

doi:10.1097/00000637-199505000-00013

Cited by 83 publications

(47 citation statements)

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“…Oral keratinocytes were isolated as described previously [Hata et al, 1995]. Briefly, the surface epithelium was harvested from oral mucosa of the mandible of the same pig from which enamel organs were removed.…”

Section: Methodsmentioning

confidence: 99%

“…Basal layer keratinocytes are progenitor cells that undergo terminal differentiation as they migrate to the surface of the oral mucosa. These cells have been successfully cultured over a long term using 3T3-J2 feeder layer cells (3T3) [Rheinwald and Green, 1975;Hata et al, 1995]. Here we describe the successful application of a 3T3 feeder layer to culture EOE cells over the long term, in effect replicating the mucosal epithelium, and the subsequent tissue engineering of enamel from these cells.…”

Section: Introductionmentioning

confidence: 99%

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Enamel Tissue Engineering Using Subcultured Enamel Organ Epithelial Cells in Combination with Dental Pulp Cells

Honda

Shinmura

Shinohara³

2008

Cells Tissues Organs

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We describe a strategy for the in vitro engineering of enamel tissue using a novel technique for culturing enamel organ epithelial (EOE) cells isolated from the enamel organ using 3T3-J2 cells as a feeder layer. These subcultured EOE cells retain the capacity to produce enamel structures over a period of extended culture. In brief, enamel organs from 6-month-old porcine third molars were dissociated into single cells and subcultured on 3T3-J2 feeder cell layers. These subcultured EOE cells were then seeded onto a collagen sponge in combination with primary dental pulp cells isolated at an early stage of crown formation, and these constructs were transplanted into athymic rats. After 4 weeks, complex enamel-dentin structures were detected in the implants. These results show that our culture technique maintained ameloblast lineage cells that were able to produce enamel in vivo. This novel subculture technique provides an important tool for tooth tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enamel Tissue Engineering Using Subcultured Enamel Organ Epithelial Cells in Combination with Dental Pulp Cells

Honda

Shinmura

Shinohara³

2008

Cells Tissues Organs

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“…What makes these cells suitable for use in ocular surface reconstruction is that they are less differentiated than epidermal keratinocytes, need less time to grow in culture, and do not undergo keratinization when maintained in culture; in addition, scarring of the biopsy location is inconspicuous [29,30], and, most important, they are devoid of secondary structures such as hair follicles and sweat glands. Transplantation of sheets of oral epithelium onto the abraded corneas of rabbits was shown to generate a clear ocular surface [31].…”

Section: Oral Mucosamentioning

confidence: 99%

Concise Review: The Coming of Age of Stem Cell Treatment for Corneal Surface Damage

Ramachandran

Basu

Sangwan

et al. 2014

Stem Cells Translational Medicine

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The cornea is a vital component of the eye because it provides approximately 70% of the refraction and focusing of incoming light. Being the outermost surface of the eye, it faces continuous stress from dryness, photodamage, infection, and injury; however, like the skin, the cornea regularly refreshes itself by shedding its epithelial cells, which are readily replaced, keeping the ocular surface stable and functional. This regular turnover of the corneal epithelial cells occurs through the stem cells in the limbus, an annular ring of a tissue surrounding the cornea, separating it from the sclera and the conjunctival membrane. The loss of this reserve of stem cells leads to a condition called limbal stem cell deficiency. Treatment for this disorder has evolved from transplanting whole limbal tissues to the affected eye to transplanting laboratory cultured limbal cells. This procedure is called cultivated limbal epithelial transplantation (CLET). Since its start in 1997, more than 1,000 CLET procedures have been reported from around the world, with varying degrees of success. In this paper, we compare the methods of cultivation and the outcomes and discuss some problem areas, use of other cells as substitutes for limbal epithelium, and various carrier materials used in transplantation. Our analysis suggests that CLET as a treatment for corneal surface damage has come of age. We also highlight a simpler procedure (simple limbal epithelial transplantation) that involves cultivation of limbal tissue in situ on the surface of the cornea in vivo and that has outcomes comparable to CLET.

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“…The main concept is to use the smooth surface of the oral mucosa, with its stem cell properties, to reconstruct the ocular surface. Oral mucosae are thought to be at a lower stage of differentiation than epidermal keratinocytes (Collin et al, 1992, Schermer et al, 1986 because they divide rapidly and can be maintained in culture for prolonged periods without keratinization (Hata et al, 1995). Various antimicrobial peptides (AMPs) are known to be present on the epithelial cells of ocular and oral surfaces (Haynes et al, 1999).…”

Section: Oral Mucosal Cellsmentioning

confidence: 99%

Limbal Stem Cell Transplantation and Corneal Neovascularization

Katikireddy¹,

Jurkunas²

2012

New Advances in Stem Cell Transplantation

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The Characteristics of Cultured Mucosal Cell Sheet as a Material for Grafting; Comparison with Cultured Epidermal Cell Sheet

Cited by 83 publications

References 0 publications

Enamel Tissue Engineering Using Subcultured Enamel Organ Epithelial Cells in Combination with Dental Pulp Cells

Enamel Tissue Engineering Using Subcultured Enamel Organ Epithelial Cells in Combination with Dental Pulp Cells

Concise Review: The Coming of Age of Stem Cell Treatment for Corneal Surface Damage

Limbal Stem Cell Transplantation and Corneal Neovascularization

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