The Characteristics of Cultural Conditions for the Mycelial Growth and Fruiting Body Formation of Paecilomyces sinclairii

The Optimal Culture Conditions Affecting the Mycelial Growth and Fruiting Body Formation ofPaecilomyces fumosoroseus

“…Of 10 culture media, Hamada medium was the most suitable for a favorable growth of P. fumosoroseus (Table 2). This result is corresponded with that of P. sinclairii which had been reported by Shim et al (2003). Even though the mycelial growth of P. fumosoroseus and P. sinclairii was optimal on Hamada medium, the mycelial growth of Grifola umbellata was very poor on Hamada medium.…”

“…Choi et al (1999) reported that mycelial growth of P. japonica was optimal at pH 7. Shim et al (2003) also reported that P. sinclairii showed maximal mycelial growth at pH 8. However, the mycelial growth and density of P. fumosoroseus was almost identical in the range of pH 6~9 (Fig.…”

“…The mycelial density of P. fumosoroseus showed thin appearances in all carbon source, whereas compact appearances were observed in 16 nitrogen sources. Shim et al (2003) also reported that P. sinclairii grew very well in basal medium which contained dextrin and glutamine, respectively. Since P. fumosoroseus and P. sinclairii belong to genus Paecilomyces of Hyphomycetes, Deuteromycota, they are taxonomically considered as very close species (Lacey et al, 1999).…”

“…Effect of C/N ratio: Optimum C/N ratio suitable for a favorable growth of P. fumosoroseus was observed on the culture media which were adjusted to C/N ratio of 40:1. On the culture media which were mixed with 1% glucose as carbon source and then adjusted to the ratio of 30:1, P. sinclairii showed the most favorable mycelial growth (Shim et al, 2003). Despite a gradual rise of 1%, 2%, 3% and 4% glucose, C/N ratio of 40:1 showed the most favorable mycelial growth of P. fumosoroseus as compared with other C/N ratios (Table 5).…”

“…Therefore, these entomopathogenic properties have led to the development (Jackson et al, 1997;Lee et al, 1999) and commercialization (Bolckmans et al, 1995;Furuya et al, 1983;Manabe et al, 1996) of mycoinsecticide or medicine. However, only a few species of Paecilomyces have been artificially cultured in Korea (Sung et al, 1997;Lee et al, 1999;Kim et al, 2002;Cho et al, 2002;Shim et al, 2003). To develop P. fumosoroseus as a mycoinsecticide for biological control or medicine for human beings, it is necessary to develop method for mass production of fruiting bodies in in vitro condition.…”