The characteristic regulation of gene expression <i>Lbp</i> and <i>Sod3</i> in peri‐implant connective tissue of rats

Kobayashi, Takafumi; Sasaki, Hodaka; Asami, Yosuke; Mori, Gentaro; Yoshinari, Masao; Yajima, Yasutomo

doi:10.1002/jbm.a.36839

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Previous animal studies have reported that OTM increased ROS-mediated oxidative stress in rat periodontal tissues (71), and rats with high gingival oxidative stress levels have lower SOD3 expression in gingival tissues with increased alveolar bone resorption (57). Increased ROS and decreased SOD3 expression in rat peri-implant connective tissues have also been observed following dental implant placement (72). Furthermore, excessive ROS in osteoblasts suppresses cell viability, SOD3 expression, and bone formation-related genes (56).…”

Section: Chapter V Discussionmentioning

confidence: 99%

Expression of superoxide dismutase 3 (SOD3) during orthodontic tooth movement in rats

Jindarojanakul

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Hypoxia induces reactive oxygen species (ROS) production in periodontal tissues. Superoxide dismutase 3 (SOD3) is an enzyme protecting�cells from ROS. This study investigated SOD3 expression and function during rat orthodontic tooth movement�(OTM) and in hypoxia-exposed rat PDL�cells. OTM of right maxillary first molars were performed in Sprague-Dawley rats for 1 and 14 days (n�= 6 per group). SOD3 and HIF-1? expression were evaluated by immunohistochemistry. SOD3 effects on cell viability and proliferation, ROS production, and mRNA expression of�Hif-1?,�Rankl,�and�Opg�in PDL cells and osteoclast differentiation were investigated under normoxia�and hypoxia. SOD3 expression in PDL tissues decreased on compression side on day 1 and on both sides on day 14. HIF-1? levels significantly increased on compression side on day 14. Cell viability, cell proliferation, and�Opg�mRNA expression decreased, whereas ROS production and�Hif-1?�and�Rankl�mRNA expression increased in SOD3-silenced PDL cells. Hypoxia reduced�Sod3�and�Opg�mRNA expression and increased ROS,�Rankl�mRNA expression, and osteoclast formation; SOD3 treatment attenuated these effects. Therefore, SOD3 plays a role in periodontal remodelling during OTM and in hypoxia-exposed PDL cells through ROS, HIF-1?, and RANKL/OPG pathways.

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Section: Chapter V Discussionmentioning

confidence: 99%

Expression of superoxide dismutase 3 (SOD3) during orthodontic tooth movement in rats

Jindarojanakul

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Dental implant material related changes in molecular signatures in peri-implantitis – A systematic review of omics in-vivo studies

Spinell,

Kröger,

Freitag

et al. 2023

Dental Materials

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Changes in superoxide dismutase 3 (SOD3) expression in periodontal tissue during orthodontic tooth movement of rat molars and the effect of SOD3 onin vitrohypoxia-exposed rat periodontal ligament cells

Jindarojanakul

Kamimoto

Niki

et al. 2023

European Journal of Orthodontics

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Summary Background/Objectives Hypoxia during orthodontic tooth movement (OTM) induces reactive oxygen species (ROS) production in periodontal tissues. Superoxide dismutase 3 (SOD3) is an anti-inflammatory enzyme that protects cells from ROS. This study investigated the expression and function of SOD3 during rat OTM and in hypoxia-exposed rat periodontal ligament (PDL) cells. Materials/Methods OTM of right maxillary first molars were performed in 8-week-old male Sprague–Dawley rats using closed-coil spring for 1 and 14 days (n = 6 per group). SOD3 and hypoxia-inducible factor 1-alpha (HIF-1α) protein expression was evaluated by immunohistochemistry. The effects of SOD3 on cell viability and proliferation, ROS production, and mRNA expression of Hif1-α, receptor activator of nuclear factor kappa-Β ligand (Rankl), and osteoprotegerin (Opg) in PDL cells and osteoclast differentiation were investigated under normal and hypoxic conditions. Results SOD3 expression in PDL tissues significantly decreased on the compression side on day 1 and on both sides on day 14 of OTM. HIF-1α levels significantly increased on the compression side on day 14. Cell viability, cell proliferation, and Opg mRNA expression decreased, whereas ROS production and Hif1-α and Rankl mRNA expression increased in the PDL cells upon SOD3 silencing. Hypoxia reduced Sod3 and Opg mRNA expression and increased ROS, Rankl mRNA expression, and osteoclast formation; SOD3 treatment attenuated these effects. Conclusion/Implications SOD3 plays a role in periodontal tissue remodelling during OTM and in hypoxia-exposed PDL cells through ROS, HIF-1α, and RANKL/OPG pathways. Moreover, SOD3 treatment could attenuate the negative effects of hypoxia on the PDL cells.

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The characteristic regulation of gene expression Lbp and Sod3 in peri‐implant connective tissue of rats

Cited by 3 publications

References 38 publications

Expression of superoxide dismutase 3 (SOD3) during orthodontic tooth movement in rats

Expression of superoxide dismutase 3 (SOD3) during orthodontic tooth movement in rats

Dental implant material related changes in molecular signatures in peri-implantitis – A systematic review of omics in-vivo studies

Changes in superoxide dismutase 3 (SOD3) expression in periodontal tissue during orthodontic tooth movement of rat molars and the effect of SOD3 onin vitrohypoxia-exposed rat periodontal ligament cells

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