The characterisation of thiobarbituric acid reactivity in human plasma and urine

Gutteridge, John M.C.; Tickner, T.R.

doi:10.1016/0003-2697(78)90838-2

Cited by 104 publications

(30 citation statements)

References 15 publications

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“…Several lines of evidences suggest an important role of enhanced lipid peroxidation in pathogenesis of hemolytic anemia. Increased level of erythrocyte MDA and decreased level of Hb have been described in experimental visceral leishmaniasis in Hamsters by Sen et al (7).The increased MDA in erythrocytes may be seen as a result of tissue catabolism of PUFAs (8). HMDA levels were significantly decreased after the treatment of patient which suggests decrease in the production of ROS due to killing of microorganisms.…”

Section: Resultsmentioning

confidence: 81%

Erythrocyte glutathione status in human visceral leishmaniasis

Neupane

Majhi

Chandra

et al. 2008

Indian J Clin Biochem

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A glutathione redox cycle is a major antioxidant defense system for the detoxification of reactive oxygen species within erythrocytes. Reactive oxygen species such as superoxide anions, hydrogen peroxide and hydroxyl radicals are generated as a host defense mechanism for killing of engulfed Leishmania donovani, a causative agent of visceral leishmaniasis, are capable of damaging lipids and other biomolecules when produced in excess. Erythrocytes are most vulnerable to Reactive oxygen species. In present study we aimed to evaluate erythrocyte reduced glutathione (GSH) levels as an antioxidant and erythrocyte malondialdehyde (MDA) as a marker of lipid peroxidation. The study included twenty-five Visceral leishmaniasis patients and they were followed up after their complete chemotherapy with antileishmanial drugs (sodium stibogluconate) for 30 days. Forty six age and sex matched healthy individuals were taken as controls. GSH levels in erythrocytes of visceral leishmaniasis patients were increased in spite of significant increased erythrocyte MDA as compared to controls. Whereas erythrocyte GSH and MDA levels of follow up patients were decreased as compared to patients before treatment groups. We concluded that visceral leishmaniasis patients are in oxidative stress which most likely induces the endogenous antioxidant such as GSH or its poor utilization by cells.

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Section: Resultsmentioning

confidence: 81%

Erythrocyte glutathione status in human visceral leishmaniasis

Neupane

Majhi

Chandra

et al. 2008

Indian J Clin Biochem

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“…Gutteridge and Tickner [1] have suggested that non volatile primary peroxidation products derived from poly unsaturated fatty acids (PUFA) are responsible for the TBA-reactive material in normal human blood. They have suggested that the TBA reactivity of blood could be a measure of overall peroxidative changes in blood lipids.…”

Section: Discussionmentioning

confidence: 99%

Thiobarbituric Acid Reactive Material in Uremic Blood

Fillit¹,

Elion²,

Sullivan³

et al. 1981

Nephron

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The thiobarbituric acid (TBA) assay was applied to uremic blood. Significant TBA-reactive material was found in patients with chronic renal failure compared to normal controls. The presence of TBA-reactive material correlated with the degree of renal failure, and was removed by hemodialysis. The reactive material is not 2-deoxyribose or sialic acid. In addition, the presence of TBA-reactive material in uremic blood is not abolished by prior aspirin ingestion, suggesting it is not related to active prostaglandin synthesis. The possible origin of the TBA-reactive material in uremic blood is discussed.

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“…TBARS were measured fluorometrically according to McMillan et al [13] with some modifications of the usual reaction procedures [9,14], The reaction tem perature was lowered to 70'C and reaction time con- T8A-MDA, pmol/l fined to 15 min in order to minimize reaction of inulin with thiobarbituric acid and to prevent unspecific hydrolysis of sugars. Standards were prepared by hydrolysis of 1,1,3,3-tetracthoxypropane according to Gutteridge [ 15], Arteriovenous differences (AV Diff) for TBARS were calculated to determine renal net excretion or absorption of these substances.…”

Section: Measurement O F Lpomentioning

confidence: 99%

“…the determination of MDA with the photometric TBA assays [6,9,15], suffer from some uncer tainties [10]. The usually applied high reac tion temperatures (100"C for 10-60 min) will produce hydrolysis products of sugars [19] and lipids, which react with TBA and deliver elevated MDA measures [9], In order to over come this problem we used a fluorimetric method [13] in combination with a lower reaction temperature. Furthermore, in a pa rallel set of experiments we controlled the MDA concentrations in blood and urine in the form of MDA-TBA adducts using HPLC [16].…”

Section: Renal Function and Lipid Peroxidation After Maleate Adminstrmentioning

confidence: 99%

Lipid Peroxidation – An Initial Event in Experimental Acute Renal Failure

Joannidis

Bonn²,

Pfaller³

1989

Kidney Blood Press Res

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A method was developed to monitor the occurrence of lipid peroxidation (LPO) during ischemia and Na-maleate-induced acute renal failure (ARF) on male rats in vivo by measuring malondialdehyde (MDA) levels in arterial and renal venous blood and in urine. No signs of LPO could be detected under control conditions. In ischemic ARF produced by 45 min of renal artery clamping a steep increase of MDA was found in the renal venous effluent immediately after starting reperfusion. This effect was nearly abolished after 5 min of blood reflow while glomerular filtration remained at 5% of control value during a 90-min postischemic observation period. Intoxication with Na-maleate leads to enhanced LPO in combination with an impaired renal function 2 h after administration. These findings would well explain cellular damage and some aspects of renal dysfunction associated with the initation phase of ARF.

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The characterisation of thiobarbituric acid reactivity in human plasma and urine

Cited by 104 publications

References 15 publications

Erythrocyte glutathione status in human visceral leishmaniasis

Erythrocyte glutathione status in human visceral leishmaniasis

Thiobarbituric Acid Reactive Material in Uremic Blood

Lipid Peroxidation – An Initial Event in Experimental Acute Renal Failure

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