The Characterisation of Pluripotent and Multipotent Stem Cells Using Fourier Transform Infrared Microspectroscopy

Abstract

Fourier transform infrared (FTIR) microspectroscopy shows potential as a benign, objective and rapid tool to screen pluripotent and multipotent stem cells for clinical use. It offers a new experimental approach that provides a holistic measurement of macromolecular composition such that a signature representing the internal cellular phenotype is obtained. The use of this technique therefore contributes information that is complementary to that acquired by conventional genetic and immunohistochemical methods.

Cellular injury evidenced by impedance technology and infrared microspectroscopy

“…Zelig et al (2009) found that this scenario to be associated with apoptotic cells [29]. [49] Other vibrational bands associated with cytotoxicity was also detected in the cells treated with 18 µg/mL (Fig. 4, the vibrational mode of the C-O-C ring of deoxyribose in Z-form DNA [37,38,44].…”

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

“…5. An absorption band at 1745 cm −1 is related to the stretching vibration of C=O ester group of lipids [21], while two intense bands at 1650 and 1540 cm −1 are related to amide I and amide II functional groups of proteins respectively [22]. Another band at 1454 cm −1 arises from the methyl and methylene groups from lipids and proteins, whereas the band at 1396 cm −1 is due to the COO − stretching vibrations of amino acid side chains [21].…”

“…An absorption band at 1745 cm −1 is related to the stretching vibration of C=O ester group of lipids [21], while two intense bands at 1650 and 1540 cm −1 are related to amide I and amide II functional groups of proteins respectively [22]. Another band at 1454 cm −1 arises from the methyl and methylene groups from lipids and proteins, whereas the band at 1396 cm −1 is due to the COO − stretching vibrations of amino acid side chains [21]. The band at 1239 cm −1 is related to the P=O asymmetrical stretching of PO 2 phosphodiester groups from phosphorylated molecules, whereas the band at 1080 cm −1 arises from the P=O symmetrical stretching of PO 2 phosphodiester groups from phosphorylated molecules and glycogen [21, 23].…”

Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

“…y Cao y cols. [8,9] , los espectros FTIR obtenidos en este trabajo de CMP y CPD fueron complejos, pero el uso del análisis multivariado nos facilitó el procesamiento para identificar y evidenciar los cambios espectrales más significativos durante la diferenciación; en nuestro caso, la segunda derivada de los espectros de CMP y CPD (Figura 4), fue utilizada para proporcionar mayor precisión al ACP.…”