The Chaperonins of Synechocystis PCC 6803 Differ in Heat Inducibility and Chaperone Activity

Kovács, Eszter; Vies, Saskia M. van der; Glatz, Attila; Török, Zsolt; Varvasovszki, Viktória; Horváth, Ibolya; Vı́gh, László

doi:10.1006/bbrc.2001.6083

Cited by 37 publications

(36 citation statements)

References 37 publications

(37 reference statements)

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“…GroEL and Cpn60 stably associate with thylakoid membranes in Anabaena strain PCC7120 (14) and in Synechocystis sp. strain PCC6803 (15). In Synechocystis sp.…”

mentioning

confidence: 99%

Overexpression of the groESL Operon Enhances the Heat and Salinity Stress Tolerance of the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC7120

Chaurasia

Apte

2009

Appl Environ Microbiol

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The bicistronic groESL operon, encoding the Hsp60 and Hsp10 chaperonins, was cloned into an integrative expression vector, pFPN, and incorporated at an innocuous site in the Anabaena sp. strain PCC7120 genome. In the recombinant Anabaena strain, the additional groESL operon was expressed from a strong cyanobacterial P psbA1 promoter without hampering the stress-responsive expression of the native groESL operon. The net expression of the two groESL operons promoted better growth, supported the vital activities of nitrogen fixation and photosynthesis at ambient conditions, and enhanced the tolerance of the recombinant Anabaena strain to heat and salinity stresses.

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“…GroEL and Cpn60 stably associate with thylakoid membranes in Anabaena strain PCC7120 (14) and in Synechocystis sp. strain PCC6803 (15). In Synechocystis sp.…”

mentioning

confidence: 99%

Overexpression of the groESL Operon Enhances the Heat and Salinity Stress Tolerance of the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC7120

Chaurasia

Apte

2009

Appl Environ Microbiol

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“…16) A GroEL tetradecamer from this cyanobacterium was detected only on native PAGE with a gel containing 17% glycerol, while without glycerol, 7-mer and monomer were detected. 16) To evaluate the stability of S. elongatus PCC 7942 native GroELs, we analyzed oligomeric forms of GroEL in crude soluble protein extracts from the S. elongatus PCC 7942 wild type and the ÁgroEL2 mutant by native PAGE, followed by Western blot analysis. We used cell extracts of both the wild type and the ÁgroEL2 mutant in order to discriminate GroEL1 from GroEL2 on the gel.…”

Section: Oligomeric States Of Cyanobacterial Groels In Cell Extractsmentioning

confidence: 85%

“…PCC 6803 groEL1 gene, but not with groEL2 of these thermophilic and mesophilic cyanobacteria. 11,16,17) Although GroEL1 is present, GroEL2, which is dispensable under normal conditions, becomes essential under heat and cold stress in Thermosynechococcus elongatus. 15) Thus GroEL1 and GroEL2 may be non-replaceable with each other.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Biochemical Characterization of Two GroEL Homologs from the CyanobacteriumSynechococcus elongatusPCC 7942

Huq

Sueoka

Narumi

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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“…2c) was exclusively found in the mutant cells (Table 1). The GroEL/GroES system is a major chaperone system in all bacteria and its involvement in cyanobacterial stress responses has been extensively studied (Hihara et al, 2001;Kovacs et al, 2001;Mary et al, 2004). The twofold upshift of periplasmic protease (SSP 8503) was only obvious for the Ftn2 mutant (Table 1).…”

Section: Protein Synthesis and Processing (Group 2)mentioning

confidence: 99%

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942

2007

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Bacterial cell division is a highly co-ordinated and fine-tuned process. In the unicellular cyanobacterium Synechococcus sp. strain PCC 7942, inactivating mutations in the ftn2 and ftn6 genes block cell division and result in a phenotype with extensively elongated cells. In order to establish the pleiotropic responses induced and cellular processes affected by blocked cell division, the proteomes of wild-type and the cell division mutants Ftn2 and Ftn6 of Synechococcus sp. strain PCC 7942 were characterized and compared. By separating soluble extracted proteins on 2D gels, more than 800 protein spots were visualized on each SYPRO Ruby-stained gel. Quantitative differences in protein composition were detected by using the PDQuest software, and comparative analysis revealed that 76 protein spots changed significantly in the cell division mutants. These protein spots were selected for identification using peptide mass fingerprints generated by MALDI-TOF MS. Fifty-three protein spots were successfully identified, representing 44 different proteins. The upregulated proteins include proteins involved in cell division/cell morphogenesis, protein synthesis and processing, oxidative stress response, amino acid metabolism, nucleotide biosynthesis, and glycolysis, as well as unknown proteins. Among the downregulated proteins are those involved in chromosome segregation, protein processing, photosynthesis, redox regulation, carbon dioxide fixation, nucleotide biosynthesis, the biosynthetic pathway to fatty acids, and energy production. Besides eliciting common responses, inactivation of Ftn2 and Ftn6 in the mutants may result in different responses in protein levels between the mutants. Among 18 identified differentially affected protein spots, 75 % (9/12) of the protein spots affected in the Ftn2 mutant were upshifted, whereas in the Ftn6 mutant 70 % (7/10) of the affected protein spots were downshifted. Identification of such differentially expressed proteins provides new targets for future studies that will allow assessment of their physiological roles and significance in cyanobacterial cell division.

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The Chaperonins of Synechocystis PCC 6803 Differ in Heat Inducibility and Chaperone Activity

Cited by 37 publications

References 37 publications

Overexpression of the groESL Operon Enhances the Heat and Salinity Stress Tolerance of the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC7120

Overexpression of the groESL Operon Enhances the Heat and Salinity Stress Tolerance of the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC7120

Comparative Biochemical Characterization of Two GroEL Homologs from the CyanobacteriumSynechococcus elongatusPCC 7942

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942

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