The Changes of Gene Expression in Honeybee (Apis mellifera) Brains Associated with Ages

Tsuchimoto, Mayumi; Aoki, Makoto; Takada, Mamoru; Kanou, Yoshinori; Sasagawa, Hiromi; Kitagawa, Yasuo; Kadowaki, Tatsuhiko

doi:10.2108/0289-0003(2004)21[23:tcogei]2.0.co;2

Cited by 23 publications

(25 citation statements)

References 37 publications

(33 reference statements)

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“…The corresponding insect-specific genes are therefore supposedly involved in more specialized functions. A similar conclusion can be reached from the micro- and macroarray analyses of transcripts detected in adult honey bee workers performing different tasks during their adult life cycle [6,7]. …”

Section: Resultssupporting

confidence: 67%

The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome

et al. 2004

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Section: Resultssupporting

confidence: 67%

The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome

et al. 2004

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“…Transcription of several genes encoding neurotransmitter transporters, ion channels, protein kinases, and phosphatases, e.g., is enhanced in the foraging bee (Tsuchimoto et al, 2004). Oxidative stress inactivates particular proteins preferentially (Sohal et al, 1990(Sohal et al, , 1995Sohal and Weindruch, 1996;Sohal, 2002).…”

Section: Discussionmentioning

confidence: 99%

Cellular senescence in honey bee brain is largely independent of chronological age

Seehuus

Krekling

Amdam

2006

Experimental Gerontology

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Accumulation of oxidative stress-induced damage in brain tissue plays an important role in the pathogenesis of normal aging and neurodegenerative diseases. Neuronal oxidative damage typically increases with age in humans, and also in the invertebrate and vertebrate model species most commonly used in aging research. By use of quantitative immunohistochemistry and Western blot, we show that this aspect of brain senescence is largely decoupled from chronological age in the honey bee (Apis mellifera). The bee is a eusocial insect characterized by the presence of a reproductive queen caste and a caste of functionally sterile female workers that performs various alloparental tasks such as nursing and foraging. We studied patterns of oxidative nitration and carbonylation damage in the brain of worker bees that performed nurse tasks as 8-and 200-day-olds and foraging tasks as 20-and 200-day-olds. In addition, we examined 180-day-old diutinus bees, a stress-resistant temporal worker form that survives unfavorable periods. Our results indicate that nitration damage occurs only at low levels in vivo, but that a 60-kDa protein from honey bee brain is selectively nitrated by peroxynitrite in vitro. Oxidative carbonylation is present at varying levels in the visual and chemosensory neuropiles of worker bees, and this inter-individual variation is better explained by social role than by chronological age.

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“…In the latter class is foraging (for), a previously identified cGMP-dependent protein kinase whose pharmacological activation causes precocious positive phototaxis and precocious foraging (Ben-Shahar et al, 2002. Similarly, a recent macroarray study demonstrates higher expression levels for genes involved in signal transduction, ion channels, neurotransmitter transport, transcription factors, plasma membrane proteins and most cell adhesion proteins in foragers as compared with newly emerged bees, suggesting plasticity and remodeling of neurocellular properties during aging and/or behavioral development in honey bees (Tsuchimoto et al, 2004). Using an earlier nonnormalized version of the honey bee brain cDNA library later used to develop the bee EST database, Kucharski and Maleszka (2002) showed that foragers also increase the expression of genes encoding royal jelly proteins, metabolic enzymes (␣-glucosidase, aminopeptidases, glucose dehydrogenase) and a LIM domain protein that is a putative transcription regulator.…”

Section: Honey Bee Behavioral Geneticsmentioning

confidence: 93%

Muscle biochemistry and the ontogeny of flight capacity during behavioral development in the honey bee,Apis mellifera

Roberts

Elekonich

2005

Journal of Experimental Biology

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Summary CommentaryMuscle biochemistry and the ontogeny of flight capacity during behavioral development in the honey bee, Apis mellifera However, the honey bee, Apis mellifera, is a model system whose experimental tractability is powerful and rapidly evolving, making this species among the best available for the study of social behavior and development. Indeed, honey bee evolution, behavior, physiology and genetics are each well represented in an abundant literature (>5000 references) on this species. Honey bees are oviparous (egg laying), holometabolous (completely metamorphosing) insects that live in large colonies usually containing over 20·000 individuals. Embryos and larvae are individually housed in open cells on honeycombs and are cared for by adult bees. The environment of the brood is maintained by the bees and is remarkably constant. The temperature is maintained between 30 and 35°C, carbon dioxide levels are held between 1 and 4.3%, and relative humidity is regulated between 70 and 75% (Winston, 1987). Honey bees exhibit a form of behavioral development termed 'temporal polyethism', moving through a series of behaviorally defined life history stages in an age-related fashion. For the first 2-3·weeks of life, adult workers perform tasks inside the hive such as brood care ('nursing') and hive maintenance. Typically at about 3·weeks of age, workers transition to performing tasks outside the hive such as foraging. Foraging bees are typically the oldest workers in the hive. The physiology of honey bees changes as they age and move from non-flying tasks in the hive to foraging, which imparts a suite of different functional demands. For example, hypopharyngeal glands regress and produce enzymes for processing nectar instead of brood food, juvenile hormone levels increase, body mass decreases, body water content increases and, as we describe in more detail below, metabolic and flight capacity increases (Fluri et al., 1982; Harrison, 1986; Huang et al., 1994;Ohashi et al., 1996Ohashi et al., , 1999Pontoh and Low, 2002;Robinson and Vargo, 1997;Winston, 1987).Many behavioral transitions made as animals enter a new environment or life history stage in adulthood typically occur only once, but honey bee adult behavioral development is exceedingly plastic and responsive to the social environment; honey bees can move into a later stage precociously, delay the transition or return to a previous behavior (and the previous physiological state associated with that behavior) depending on the social context and colony needs. For example, in colonies deficient in nurses, young bees will continue to tend brood rather than switch to outside tasks (Robinson et al., 1989). Similarly, in colonies completely lacking young bees, older bees that would normally be foragers often revert to nursing behavior. In reverted nurses, juvenile hormone levels drop and their hypopharyngeal glands enlarge to resemble those of normally aged nurses (Huang and Robinson, 1996;Page et al., 1992;Robinson et al., 1992). The effect of colony demograph...

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The Changes of Gene Expression in Honeybee (Apis mellifera) Brains Associated with Ages

Cited by 23 publications

References 37 publications

The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome

The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome

Cellular senescence in honey bee brain is largely independent of chronological age

Muscle biochemistry and the ontogeny of flight capacity during behavioral development in the honey bee,Apis mellifera

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