The changes in electrolytes, particularly chloride, which accompany growth in chick muscle

Barlow, Joseph J.; Manery, J. F.

doi:10.1002/jcp.1030430204

Cited by 27 publications

(10 citation statements)

References 34 publications

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“…On the other hand, the intracellular sodium concentration fell significantly over the 5 days of culture (P<O*Ol). The value obtained for myoblasts is almost the same as that reported by Barlow & Manery (1954) for 34-day chick, where fusion into muscle fibres had already occurred. Apparently, therefore, the rate of fall of intracellular sodium concentration is more rapid in cultured muscle than in the intact animal.…”

Section: Distribution Of Ionssupporting

confidence: 88%

Properties of the Cell Membrane of Developing Skeletal Muscle Fibres in Culture and Its Sensitivity to Acetylcholine

Dryden

Erulkar

Haba

1974

Clin Exp Pharmacol Physiol

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S U M M A R Y 1. The relationship between determinants of membrane potential has been studied during myogenesis in culture.2. Myoblasts obtained from 1 1-day chick embryo musculature were cultured and intracellular records were made from myoblasts and developing unstriated and striated myotubes.3. Resting membrane potentials increased with time of culture until values equivalent to those obtained from fully developed striated muscle fibres were reached after about 20 days in culture.4. High concentrations of potassium added to the medium failed to affect significantly the resting membrane potentials in myoblasts and myotubes up to 10 days in culture. Changes in external sodium had little effect on values of potentials from myotubes and cells up to 4 days in culture, but removal of sodium after 5 and 6 days in culture caused depolarization. 5. Electrolyte distributions determined for myoblasts and myotubes 4 days after fusion showed no significant change in potassium content, whereas the sodium concentration fell significantly. 6. Acetylcholine applied by microinjection caused two types of response from myoblasts depending upon the level of the resting membrane potential : when the potential was less than -7 mV, hyperpolarization occurred; when it was greater than this value, depolarization occurred. 7. Depolarizing responses to acetylcholine application were regularly obtained from myotubes with resting membrane potentials greater than -7 mV.8. The responses to acetylcholine could be blocked by d-tubocurarine in the medium ; tetramethylammonium (a nicotinic agonist) acted similarly to acetylcholine, but bethanecol (a muscarinic agonist) had no effect.

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Section: Distribution Of Ionssupporting

confidence: 88%

Properties of the Cell Membrane of Developing Skeletal Muscle Fibres in Culture and Its Sensitivity to Acetylcholine

Dryden

Erulkar

Haba

1974

Clin Exp Pharmacol Physiol

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“…Intracellular concentration of elements in rabbit psoas muscle is given in Table 1 Apparently there is a relatively large amount of chlorine inside the muscle cell. While the relative concentration of intracellular chlorine is considerably less than in serum, (Table 1), it is much higher than anticipated (4).…”

Section: Resultsmentioning

confidence: 92%

Scanning Transmission Electron microscope Studies of Deep-Frozen Unfixed Muscle Correlated with Spatial Localization of Intracellular Elements by Fluorescent X-Ray Analysis

Bacaner

Broadhurst

Hutchinson

et al. 1973

Proc. Natl. Acad. Sci. U.S.A.

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Thin sections of deep-frozen unfixed muscle were studied in a scanning electron microscope modified for transmission imaging and equipped with a "cryostage" for vacuum compatibility of hydrated tissue.With an energy-dispersive x-ray analysis system, intracellular atomic species in the scan beam path were identified by their fluorescent x-rays and spatially localized in correlation with the electron optical image of the microstructure. Marked differences are noted between the ultrastructure of deep-frozen hydrated muscle and that of fixed dehydrated muscle. In frozen muscle, myofibrils appear to be composed of previously undescribed longitudinal structures between 400-1000 A wide ("macromyofilaments"). The usual myofilaments, mitochondria, and sarcoplasmic reticulum were not seen unless the tissue was "fixed" before examination. Fluorescent x-ray analysis of the spatial location of constituent elements clearly identified all elements heavier than Na. Intracellular Cl was relatively higher than expected.The present body of knowledge relating the microstructure and physiologic function of muscle is based primarily on (1) electron microscope studies of fixed tissue, dehydrated so as to be compatible with the vacuum required for electron optical imaging and (2) (Fig. 1).To obtain sufficient contrast in the electron optical images of a thin section of tissue, the normal reflected electron imaging operation of the SEM was converted to transmission imaging-causing it to function as a transmission scanning electron microscope. The contrast obtained in unstained unfixed tissue was enhanced by partial dark-field techniques that were developed for this purpose. A detailed description of the cryostage and transfer system and transmission imaging technique will be reported separately (16).X-Ray Analysis. In the SEM, a 20 keV electron beam focused to a small spot, about 75 A in diameter, is scanned 3423

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“…Muscular growth in birds and mammals is known to involve a fall in the percentage of water and extracellular electrolytes, and a rise in the percentage of protein, potassium and phosphorus (Leslie & Davidson, 1951;Robinson, 1952a;Barlow & Manery, 1954). McCance & found that during the development of human and pig muscle there was an increase in the nitrogen/potassium ratio.…”

mentioning

confidence: 99%

The effect of growth on the composition of avian muscle

Dickerson

1960

Biochemical Journal

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The changes in electrolytes, particularly chloride, which accompany growth in chick muscle

Cited by 27 publications

References 34 publications

Properties of the Cell Membrane of Developing Skeletal Muscle Fibres in Culture and Its Sensitivity to Acetylcholine

Properties of the Cell Membrane of Developing Skeletal Muscle Fibres in Culture and Its Sensitivity to Acetylcholine

Scanning Transmission Electron microscope Studies of Deep-Frozen Unfixed Muscle Correlated with Spatial Localization of Intracellular Elements by Fluorescent X-Ray Analysis

The effect of growth on the composition of avian muscle

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