The challenge of RNAi-mediated control of hemipterans

Christiaens, Olivier; Smagghe, Guy

doi:10.1016/j.cois.2014.09.012

Cited by 112 publications

(105 citation statements)

References 63 publications

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“…In T. Castaneum , Drosophila , and Helicoverpa armigera , the silencing of EcR and/or USP resulted in molting defects and mortality of the insect juveniles [32,34], indicating that EcR and USP indeed play an important role in development and reproduction across different insect orders. However, our result contrasts with the absences of RNAi responses in pea aphid when dsRNAs targeting EcR and USP genes were delivered through either oral feeding or injection [37]. Differences in RNAi efficacy have been widely observed between experiments and laboratories, even in different populations or strains of the same species in different labs [8,10,14,38].…”

Section: Discussioncontrasting

confidence: 66%

RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants

Yan

Chen

Sun

et al. 2016

IJMS

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RNA interference (RNAi) has been widely used in functional genomics of insects and received intensive attention in the development of RNAi-based plants for insect control. Ecdysone receptor (EcR) and ultraspiracle protein (USP) play important roles in molting, metamorphosis, and reproduction of insects. EcR and USP orthologs and their function in grain aphid (Sitobion avenae F.) have not been documented yet. Here, RT-PCR, qRT-PCR, dsRNA feeding assay and aphid bioassay were employed to isolate EcR and USP orthologs in grain aphid, investigate their expression patterns, and evaluate the effect of RNAi on aphid survival and fecundity, and its persistence. The results indicated that SaEcR and SaUSP exhibited similar expression profiles at different developmental stages. Oral administration of dsRNAs of SaEcR and dsSaUSP significantly decreased the survival of aphids due to the down-regulation of these two genes, respectively. The silencing effect was persistent and transgenerational, as demonstrated by the reduced survival and fecundity due to knock-down of SaEcR and SaUSP in both the surviving aphids and their offspring, even after switching to aphid-susceptible wheat plants. Taken together, our results demonstrate that SaEcR and SaUSP are essential genes in aphid growth and development, and could be used as RNAi targets for wheat aphid control.

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Section: Discussioncontrasting

confidence: 66%

RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants

Yan

Chen

Sun

et al. 2016

IJMS

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“…The degradation of dsRNA in the gut or salivary secretions has already been reported in several insects . Two possible reasons for dsRNA degradation are proposed: either the dsRNA is unstable at a certain pH level in the specific tissues in the insect and/or dsRNases are present in the insect digestive system . The pH range of the hemipteran gut can vary between acidic and alkali conditions, depending on the localisation in the gut, while dsRNA is unstable under very alkali conditions .…”

Section: Challenges Of Plant‐mediated Rnai For Aphid Controlmentioning

confidence: 99%

“…The dsRNA is cleaved by the RNaseIII‐enzyme Dicer into 20–25 nucleotide long, small interfering RNA (siRNA). Subsequently, Argonaute proteins assemble this siRNA to form an RNA‐induced silencing complex (RISC) that targets the destruction of the endogenous mRNA complementary to its guide strand . Several RNAi machinery components, including Dicer, Argonaute, various helicases and dsRNA endonucleases, have been identified in aphid …”

Section: Mechanisms Of Plant‐mediated Rnai Against Aphidsmentioning

confidence: 99%

RNAi‐mediated plant protection against aphids

Liu

Huang

et al. 2016

Pest Management Science

124

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Aphids (Aphididae) are major agricultural pests that cause significant yield losses of crop plants each year by inflicting damage both through the direct effects of feeding and by vectoring harmful plant viruses. Expression of double-stranded RNA (dsRNA) directed against suitable insect target genes in transgenic plants has been shown to give protection against pests through plant-mediated RNA interference (RNAi). Thus, as a potential alternative and effective strategy for insect pest management in agricultural practice, plant-mediated RNAi for aphid control has received close attention in recent years. In this review, the mechanism of RNAi in insects and the so far explored effective RNAi target genes in aphids, their potential applications in the development of transgenic plants for aphid control and the major challenges in this regard are reviewed, and the future prospects of using plant-mediated RNAi for aphid control are discussed. This review is intended to be a helpful insight into the generation of aphid-resistant plants through plant-mediated RNAi strategy. © 2016 Society of Chemical Industry.

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“…It also has the potential to be developed as an insect control strategy by targeting genes involved in growth and development . RNAi in aphids has been applied by injection of dsRNA or ingestion of a mixture of dsRNA with an artificial diet . Alternatively, dsRNA uptake through a plant leaf has been effective in the silencing of the ecdysone receptor gene in the whitefly, Bemisia tabaci , and causing nymph mortality .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a cuticle protein gene as a potential RNAi target in aphids

Shang

Ding

et al. 2019

Pest Management Science

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BACKGROUND RNA interference (RNAi) has potential as a pest insect control technique. One possible RNAi target is the cuticle protein, which is important in insect molting and development. As an example, here we evaluate the possibility of designing double‐stranded RNA (RNA) that is effective for silencing the cuticle protein 19 gene (CP19) in aphids but is harmless to non‐target predator insects. RESULTS The sequences of CP19s were similar (86.6–94.4%) among the tested aphid species (Aphis citricidus, Acyrthosiphon pisum, and Myzus persicae) but different in the predator Propylaea japonica. Ingestion of species‐specific dsRNAs of CP19 by the three aphids produced 39.3–64.2% gene silencing and 45.8–55.8% mortality. Ingestion of non‐species‐specific dsRNA (dsAcCP19) by Ac. pisum and M. persicae gave gene silencing levels ranging from 40.4% to 50.3% and 43.3–50.8% mortality. The dsApCP19 did not affect PjCP19 expression or developmental duration in P. japonica. CONCLUSION The results demonstrate that CP19 is a promising RNAi target for aphid control via one dsRNA design. The targeting of genes that are conserved in insect pests but not present in beneficial insects is a useful RNAi‐based pest control strategy. © 2019 Society of Chemical Industry

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The challenge of RNAi-mediated control of hemipterans

Cited by 112 publications

References 63 publications

RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants

RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants

RNAi‐mediated plant protection against aphids

Evaluation of a cuticle protein gene as a potential RNAi target in aphids

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