The challenge and advancement of annulus fibrosus tissue engineering

Jin, Li; Shimmer, Adam L.; Li, Xudong

doi:10.1007/s00586-013-2663-2

Cited by 34 publications

(25 citation statements)

References 109 publications

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“…Cell migration is observed more frequently in AF compared to NP, nevertheless AF's regenerative ability remains rather limited . AF is a type of tissue that comprises distinguishable and intricately designed layers, which gradually change from the inner to outer AF . High technological skills, sophisticated biomaterials, and tissue engineering methods are likely necessary to form AF tissue constituents .…”

Section: Discussionmentioning

confidence: 99%

“…39 AF is a type of tissue that comprises distinguishable and intricately designed layers, which gradually change from the inner to outer AF. 40 High technological skills, sophisticated biomaterials, and tissue engineering methods are likely necessary to form AF tissue constituents. 12 Moreover, AF tissue maintenance and repair depends on lower cell numbers than other cartilage tissues.…”

Section: Discussionmentioning

confidence: 99%

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Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

et al. 2019

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In recent years, studies have explored novel approaches for cell transplantation to enable annulus fibrosus (AF) regeneration of the intervertebral disc in particular for lumbar disc herniation. Nevertheless, successful engraftment of cells is structurally challenging, and no definitive method has yet been established. This study investigated the potential of cell sheet technology to facilitate cell engraftment for AF repair. AF injury was induced by a 1 × 1 mm defect in rat tails after which AF cell sheets were transplanted. Its regenerative effects were compared to a nondegenerated and degeneration only conditions. Degenerative changes of the entire intervertebral disc were examined by disc height measurements, histology, and immunohistochemistry for 4‐, 8‐, and 12‐weeks post‐transplantation. Cell engraftment was confirmed by tracing PKH26 fluorescent dyed AF cells. In the transplant group, disc degeneration was significantly suppressed after 4, 8, and 12 weeks when compared with the degenerative group, as indicated by histological scoring and DHI observations. At 2 and 4 weeks after transplant, PKH26 positive cells could be detected in defect region and surrounding AF. The results suggest cell engraftment into AF tissue could be established by the cell sheet technology without additional scaffolding or adhesives. In short, AF cell sheets appear to be an effective and accessible tool for AF repair and to support intervertebral disc regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

et al. 2019

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“…Therefore, engineered disc replacements must be designed to achieve similar properties to healthy disc tissues for both function in the joint and to provide mechanical signal to the residing cells. Functional tissue engineering of the intervertebral disc has focused on replacements for the NP, AF, and disc-like constructs (Bowles et al, 2012, 2011; Foss et al, 2014; Hu et al, 2012; Hudson et al, 2013; Iatridis et al, 2013; Jin et al, 2013; Mizuno et al, 2006; Nerurkar et al, 2010b, 2009; O'Halloran and Pandit, 2007; Sasson et al, 2012; Silva-Correia et al, 2011; Strange and Oyen, 2012). Tissue engineering of the AF has mainly focused on replicating its structural and tensile properties (Hudson et al, 2013; Jin et al, 2013; Nerurkar et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Elastic, permeability and swelling properties of human intervertebral disc tissues: A benchmark for tissue engineering

Cortés

Jacobs

DeLucca

et al. 2014

Journal of Biomechanics

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SUMMARY The aim of functional tissue engineering is to repair and replace tissues that have a biomechanical function, i.e., connective orthopaedic tissues. To do this, it is necessary to have accurate benchmarks for the elastic, permeability, and swelling (i.e., biphasic-swelling) properties of native tissues. However, in the case of the intervertebral disc, the biphasic-swelling properties of individual tissues reported in the literature exhibit great variation and even span several orders of magnitude. This variation is probably caused by differences in the testing protocols and the constitutive models used to analyze the data. Therefore, the objective of this study was to measure the human lumbar disc annulus fibrosus (AF), nucleus pulposus (NP), and cartilaginous endplates (CEP) biphasic-swelling properties using a consistent experimental protocol and analyses. The testing protocol was composed of a swelling period followed by multiple confined compression ramps. To analyze the confined compression data, the tissues were modeled using a biphasic-swelling model, which augments the standard biphasic model through the addition of a deformation-dependent osmotic pressure term. This model allows considering the swelling deformations and the contribution of osmotic pressure in the analysis of the experimental data. The swelling stretch was not different between the disc regions (AF: 1.28±0.16; NP: 1.73±0.74; CEP: 1.29±0.26), with a total average of 1.42. The aggregate modulus (Ha) of the matrix was higher in the CEP (390 kPa) compared to the NP (100 kPA) or AF (30 kPa). The permeability was very different across tissues regions, with the AF permeability (80 E−4 mm4/Ns) higher than the NP and CEP (6-7 E−16 m4/Ns). Additionally, a normalized time-constant (3000 sec) for the stress relaxation was similar for all the disc tissues. The properties measured in this study are important as benchmarks for tissue engineering and for modeling the disc's mechanical behavior and transport.

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“…In view of the native structure of AF tissue, restoration of the function and properties of the AF requires the use of biomaterials that are easy to process and have good mechanical properties . In recent years, scaffolds used for tissue engineered AF have included silk protein, an alginate/chitosan composite material, demineralized bone matrix and synthetic polymer materials (such as poly‐ɛ‐caprolactone [PCL]) …”

Section: Methodsmentioning

confidence: 99%

Construction Strategy and Progress of Whole Intervertebral Disc Tissue Engineering

Yang¹,

Xu²,

Hurday

et al. 2016

Orthopaedic Surgery

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Degenerative disc disease (DDD) is the major cause of low back pain, which usually leads to work absenteeism, medical visits and hospitalization. Because the current conservative procedures and surgical approaches to treatment of DDD only aim to relieve the symptoms of disease but not to regenerate the diseased disc, their long-term efficiency is limited. With the rapid developments in medical science, tissue engineering techniques have progressed markedly in recent years, providing a novel regenerative strategy for managing intervertebral disc disease. However, there are as yet no ideal methods for constructing tissue-engineered intervertebral discs. This paper reviews published reports pertaining to intervertebral disc tissue engineering and summarizes data concerning the seed cells and scaffold materials for tissue-engineered intervertebral discs, construction of tissue-engineered whole intervertebral discs, relevant animal experiments and effects of mechanics on the construction of tissue-engineered intervertebral disc and outlines the existing problems and future directions. Although the perfect regenerative strategy for treating DDD has not yet been developed, great progress has been achieved in the construction of tissue-engineered intervertebral discs. It is believed that ongoing research on intervertebral disc tissue engineering will result in revolutionary progress in the treatment of DDD.

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The challenge and advancement of annulus fibrosus tissue engineering

Cited by 34 publications

References 109 publications

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

Elastic, permeability and swelling properties of human intervertebral disc tissues: A benchmark for tissue engineering

Construction Strategy and Progress of Whole Intervertebral Disc Tissue Engineering

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