The certification of SRM 1546 - Meat Homogenate, a new reference material for nutrients in a high protein, high fat matrix

Abstract

In response to reference material needs expressed by the food industry and government regulators, the National Institute of Standards and Technology (NIST) has developed a new Standard Reference Material (SRM) consisting of a canned meat product with certified and reference values for a large number of constituents. SRM 1546 Meat Homogenate consists of a mixture of finely ground pork and chicken prepared and canned by a commercial process. NIST determined the concentration levels of cholesterol, sodium, calcium, iron, and seven fatty acids in this SRM using well defined methods and procedures. These analytes as well as 34 other constituents or properties were determined in an interlaboratory comparison exercise involving 21 laboratories, most of which are associated with the National Food Processors Association (NFPA) Food Industry Analytical Chemists Subcommittee (FIACS). From statistical analysis of the data, NIST assigned certified concentrations for the eleven analytes measured at NIST and reference concentrations for the proximates, six additional fatty acids, seven minerals, and seven water-soluble vitamins. Information values without uncertainties are provided for the concentrations of six additional constituents for which the uncertainties could not adequately be assessed. SRM 1546 will provide laboratories with a means to evaluate the accuracy of the methods they use to assign nutrient levels to processed meats and similar products.

Cholesterol Content and Methods for Cholesterol Determination in Meat and Poultry

“…Therefore, the isotopically labeled internal standard and the analyte behave similarly through all preparation and quantitative steps and co‐elute, except that they have different masses. For example, the cholesterol‐(25,26,27 13 C 3 ) (Welch and others 2001) and cholesterol‐d 7 (Ellerbe and others 1989) after TMS derivatization and electron impact ionization are monitored at m/z 461 and m/z 465, respectively, whereas the TMS derivative of food cholesterol is monitored at m/z 458 (Welch and others 2001). The information about mass and ion intensity is used to quantify analytes.…”

“…ID/MS is a highly reliable and accurate method for quantification. It has become the definitive method of analysis, which has been used to certify concentrations of food‐related analytes in SR materials (Ellerbe and others 1989; Welch and others 2001; NIST 2010). The ID/MS was initially evaluated for determining cholesterol in serum only (Gambert and others 1979; Cohen and others 1980; Takatsu and Nishi 1987).…”

“…Eckfeldt and others (1991) developed a GC‐ID/MS method for serum cholesterol with a benchtop quadrupole MS and cholesterol‐(25,26,27 13 C 3 ) as an internal standard and found that their method was as precise and accurate as the national definitive method developed by NIST using a magnetic sector MS for serum cholesterol. The studied method was also less labor intensive so that a single analyst could analyze up to 30 samples in 8 h. The ID/MS technique has been shown to be superior to other quantification methods and been the only definitive method for cholesterol analysis in foods (Ellerbe and others 1989; Welch and others 2001; NIST 2010); however, this technique is more expensive, more time‐consuming, and therefore impractical for routine analysis and is only used as reference method and for research purposes. Recent advances in MS and ID/MS techniques for the analyses of sterols and food‐related compounds were reviewed by Shimada and others (2001), Careri and others (2002), and Griffiths and Wang (2009).…”

Characterization of Three Berry Standard Reference Materials for Nutrients

“…A summary of the elemental compositions of selected food-matrix SRMs available from NIST is provided in Table 2. As has been observed previously, some laboratories have difficulty measuring low concentrations of elements in foods, e.g., sodium in SRM 2384 Baking Chocolate and iron in SRM 1546 Meat Homogenate (11, 12). Contamination in the laboratory is the probable cause of the problem.…”

Influence of Nutrient Intake on 24 Hour Urinary Hydration Biomarkers Using a Clustering-Based Approach

“…Nutrient balance plays a critical role in human development [ 1 , 2 , 3 ] and interplays with every major organ system across the stages of life [ 4 , 5 , 6 , 7 ]. The nutrient matrix, in its broad conception [ 5 , 8 ], illustrates the importance of synergistic interactions between nutrients whose co-concentration has vital implications for prevention of injury and disease [ 9 , 10 , 11 ]. Tandem consumption of certain nutrients (both macronutrients and micronutrients) creates synergies that are critical to body functions.…”