The Ceramics Radiating Far Infrared Ray Energy (Rhyolite) Promote the Formation of Bone

Abstract

Far infrared ray (FIR) energy radiated by the natural ceramics (Rhyolite) cooled FIR ceramics activates water molecules and blood circulation to stimulate skin and other tissues. The aim of our study is to make clear whether the FIR ceramics radiating FIR energy affect or not on the new bone formation in vivo and in vitro. Methods: MC3T3-E1 cells were cultured in FIR CO 2 incubator. The cell proliferation and the gene expression were analyzed by using WST-8 assay kit, RT-PCR and micro array analysis. The enzyme activities were analyzed by using the api R ZYM kit. Furthermore, titanium and natural FIR ceramics compounds were implanted under the periosteium of rat skull bone by injection method. Four weeks later, the samples were examined by the light microscope and micro CT analyses. Results: Proliferation of MC3T3-E1 cell was and DNA concentrations were inhibited by FIR energy radiation. The ALP activities were accelerated and the area of calcification nodules increased on 4 weeks. The RT-PCR data showed that the gene Runx2, Osterix, BSP, OCN, Col1a1 and OPN expression of MC3T3-E1 osteoblast like cells was activated. Bone mineral density (BMD mg/cm²) of implanted sites of T50-F50, T25-F75 and F100 groups was significantly enhanced after 4 weeks compared with control groups. This data shows FIR energy radiation by the natural FIR ceramics promoted bone-forming activity of osteoblasts. Significance: This study suggested that new bioactive ceramics such us natural FIR ceramics was useful for some clinically applications to repair bone defects for example of dental implant surgery.

Far-Infrared Irradiation Inhibits Adipogenic Differentiation and Stimulates Osteogenic Differentiation of Human Tonsil-Derived Mesenchymal Stem Cells: Role of Protein Phosphatase 2B

“…In this regard, FIR sauna therapy was reported to significantly reduce body weight and body fat in obese patients [46]. There are also a few findings of in vivo beneficial effects of FIR irradiation on bone formation; for example, FIR irradiation was reported to promote bone-forming activity of osteoblasts and increase bone mineral density when natural FIR ceramics were implanted under the periosteum of rat skull [47]. Our current data, together with these previous findings, may extend the potential use of FIR therapy in clinical applications to treat obesity and repair bone defects.…”