The cep quorum-sensing system of Burkholderia cepacia H111 controls biofilm formation and swarming motility

Huber, Birgit; Riedel, Katharina; Hentzer, Morten; Heydorn, Arne; Gotschlich, Astrid; Givskov, Michael; Molin, Søren; Eberl, Leo

doi:10.1099/00221287-147-9-2517

Cited by 403 publications

(374 citation statements)

References 67 publications

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“…The migration front is preceded by a visible layer of slime-like extracellular material as observed, for example, for Burkholderia cepacia (12,17). Within the matrix of extracellular polymeric material, the population densities are obviously extremely high (17). In support of this view, it has been demonstrated that QS is induced and AHLs are produced in swarming colonies (reviewed in ref.…”

supporting

confidence: 51%

“…Remarkable is the role of small hydrophobic proteins, SapB or the hydrophobin SC3, in the formation of Streptomyces or fungal aerial mycelia (28). QSregulated biosurfactant production via AHLs with side chains from four to six carbons has been demonstrated in Serratia liquefaciens (10), Pseudomonas aeruginosa (29,30), and possibly B. cepacia (17). Moreover, in these bacteria the link between the biosurfactant production and swarming has also been shown.…”

Section: Oh-(slc)-hsl Produced By Cini In R Etli Decreases Viscosmentioning

confidence: 94%

“…It has been suggested that glycolipid and͞or lipopeptide biosurfactants thereby produced function as wetting agents (16). The migration front is preceded by a visible layer of slime-like extracellular material as observed, for example, for Burkholderia cepacia (12,17). Within the matrix of extracellular polymeric material, the population densities are obviously extremely high (17).…”

mentioning

confidence: 99%

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Quorum signal molecules as biosurfactants affecting swarming in Rhizobium etli

Daniels

Reynaert

Hoekstra

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

138

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Swarming motility is suggested to be a social phenomenon that enables groups of bacteria to coordinately and rapidly move atop solid surfaces. This multicellular behavior, during which the apparently organized bacterial populations are embedded in an extracellular slime layer, has previously been linked with biofilm formation and virulence. Many population density-controlled activities involve the activation of complex signaling pathways using small diffusible molecules, also known as autoinducers. In Gramnegative bacteria, quorum sensing (QS) is achieved primarily by means of N-acylhomoserine lactones (AHLs). Here, we report on a dual function of AHL molecules in controlling swarming behavior of Rhizobium etli, the bacterial symbiotic partner of the common bean plant. The major swarming regulator of R. etli is the cinIR QS system, which is specifically activated in swarming cells by its cognate AHL and other long-chain AHLs. This signaling role of long-chain AHLs is required for high-level expression of the cin and rai QS systems. Besides this signaling function, the long-chain AHLs also have a direct role in surface movement of swarmer cells as these molecules possess significant surface activity and induce liquid flows, known as Marangoni flows, as a result of gradients in surface tension at biologically relevant concentrations. These results point to an as-yet-undisclosed direct role of long-chain AHL molecules as biosurfactants.cell-cell signaling ͉ motility ͉ quorum sensing

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supporting

confidence: 51%

Section: Oh-(slc)-hsl Produced By Cini In R Etli Decreases Viscosmentioning

confidence: 94%

mentioning

confidence: 99%

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Quorum signal molecules as biosurfactants affecting swarming in Rhizobium etli

Daniels

Reynaert

Hoekstra

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

138

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“…L'adhésion est la première étape, absolument nécessaire pour la formation du biofilm. Mais même si l'adhérence est suffisamment efficace pour conduire au recouvrement de la surface par des couches multiples de cellules bactériennes, il a été observé que des cellules fortement adhérentes peuvent ne pas conduire à la structure tridimensionnelle typique d'un biofilm [13]. Il a été proposé d'expliquer les propriétés adhésives des bactéries par les propriétés physicochimiques globales de la surface des cellules [4].…”

Section: Attachement Aux Surfacesunclassified

Article

Ibrahim

Briandet²,

Mistou

et al. 2004

Lait

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-Immobilization of lactococci. Bacteria present in dairy products exist predominantly in an immobilized form, as coagulated milk is not a liquid and thus does not allow bacteria to move free. Lactococci may be immobilized by attachment to surfaces, chain formation and by trapping in the extracellular matrix. Use of semi-liquid medium to simulate immobilized growth revealed characteristic properties of non-planctonic lactococcal cultures, which are: limited distribution in the medium, slow growth, a mixture of the stationary and exponentially growing cells, and genetic instability of the culture. These characteristics depend on molecular composition of the cell wall. We have shown that peptidoglycan (PG) breaks, which can be introduced not only by PG hydrolases, but also by defects of PG synthesis machinery (mutation in the ponA gene) play a significant role in immobilization (adhesion, sedimentation, chain formation). We also showed that cell wall anchored proteins play a positive role in L. lactis adhesion. Our studies contribute to the identification of molecular determinants responsible for the phenomenon of adhesion, formation of biofilms, and more generally for functioning of the lactic acid bacteria in natural and industrial environments. They have equally as objective to define the molecular factors necessary for the establishment of positive biofilms, which would minimize the risks of contamination in dairy industry. Lactococcus lactis / adhesion / biofilm / cell wall / peptidoglycan hydrolaseRésumé -Dans l'industrie laitière, les bactéries se retrouvent la plupart du temps sous une forme immobilisée, dans la mesure où le lait coagulé ne constitue pas un milieu liquide et ne permet donc pas aux bactéries en croissance des déplacements libres. Notre principal objectif réside dans la modélisation de la croissance immobilisée. Les lactocoques peuvent être immobilisés par un attachement sur les surfaces, la formation de chaînes ou l'emprisonnement au sein d'une matrice extra cellulaire. L'utilisation d'un milieu semi-liquide comme modèle d'immobilisation nous a permis de montrer que les propriétés caractéristiques de la croissance non-planctonique des lactocoques consistent en une distribution limitée dans le milieu, une croissance lente et le mélange de cellules en phases stationnaires et exponentielles, et que l'immobilisation est à l'origine de l'instabilité génétique de la culture. Ces caractéristiques dépendent de la composition moléculaire de la paroi cellulaire. Les cassures de peptidoglycane (PG), qui peuvent être introduites non seulement par des PG hydrolases, mais aussi suite à des défauts de la machinerie de synthèse du PG (mutation du gène ponA), sont importantes dans la croissance sous forme immobilisée des * Auteur correspondant : Saulius.Kulakauskas@diamant.jouy.inra.fr 104 M. Ibrahim et al.

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“…Interestingly, in some species, such as Serratia marcescens, the requirement for quorum-sensing regulation of attachment depends on the nature of the surface, with adherence to abiotic but not biotic surfaces being modulated by quorum sensing (87). Overall, the importance of quorum sensing in the regulation of cell-surface and cell-cell attachment has been repeatedly reported for a variety of other bacterial species, including E. coli, L. monocytogenes, Burkholderia cenocepacia, and Burkholderia cepacia (22,37,52,64,154).…”

Section: Figmentioning

confidence: 99%