The central part of the cyanelle rDNA unit of Cyanophora paradoxa: Sequence comparison with chloroplasts and cyanobacteria

Janssen, Ines; Mucke, Hermann Am; Löffelhardt, Wolfgang; Bohnert, Hans J.

doi:10.1007/bf00015879

Cited by 31 publications

(12 citation statements)

References 25 publications

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“…The S12-$7 intergenic space of the cyanelle operon is much smaller and shows no homology to the corresponding region on 16S rRNA [25]. This seems to rule out autoregulation in accordance with reports on respective plastid operons [ 18].…”

Section: Discussionsupporting

confidence: 68%

“…2 and 3). A putative ribosome binding site [25] is located 19 nucleotides upstream of the initiator ATG.…”

Section: Resultsmentioning

confidence: 99%

“…2 and 3). ThepsbA promoter giving also rise to a major transcript contains the classical'-35' sequence motif [25]. In contrast to the rpsl2-rps7 intergenic region a good ShineDalgarno sequence is found 11 nucleotides upstream of the tufA start codon.…”

Section: Resultsmentioning

confidence: 99%

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The cyanelle str operon from Cyanophora paradoxa: Sequence analysis and phylogenetic implications

1990

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The str operon containing the genes for the ribosomal proteins S12 (rps12) and S7 (rps7) and for the elongation factors G (fus) and Tu (tufA) has been characterized for some cyanobacteria and chloroplasts from algae and higher plants. In the case of plastids a stepwise reduction by one and two genes, respectively, has been observed due to gene transfer to the nuclear genome. The nucleotide sequence of the str operon on the cyanelle genome from Cyanophora paradoxa was determined as a first example for a chlorophyll b-less plastid. It comprises rps12, rps7 and tufA which are closely linked and not interrupted by introns. Transcript analysis revealed cotranscription of the two ribosomal protein genes whereas tufA gave rise to a monocistronic mRNA. Phylogenetic studies using these three different traits allowed an assessment of the position of Cyanophora paradoxa among oxygenic photoautotrophs.

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Section: Discussionsupporting

confidence: 68%

“…2 and 3). A putative ribosome binding site [25] is located 19 nucleotides upstream of the initiator ATG.…”

Section: Resultsmentioning

confidence: 99%

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The cyanelle str operon from Cyanophora paradoxa: Sequence analysis and phylogenetic implications

1990

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“…Further information is available from the sequences of the 16S rRNA-23S rRNA ITS domain in members of several cyanobacterial genera that include the unicellular Synechococcus PCC 6301 (Tomioka & Sugiura, 1984), Synechocystis PCC 6803 (Kaneko et al, 1996) and 47 strains of the genus Microcystis (Otsuka et al, 1999), the filamentous (non-heterocystous) Arthrospira PCC 7345 (Nelissen et al, 1994), Spirulina PCC 6313 (Nelissen et al, 1994) and Trichodesmium NIBB 1067 , the heterocystous Nodularia BCNOD 9427 (Hayes & Barker, 1997) and a strain of uncertain generic identity, ' Mastigocladus HTF ' strain PCC 7518 (G. Van der Auwera & A. Wilmotte, personal communication). In addition, the sequence of the ITS of the photosynthetic cyanelle of Cyanophora paradoxa has been determined (Janssen et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Comparison of conserved structural and regulatory domains within divergent 16S rRNA–23S rRNA spacer sequences of cyanobacteria The GenBank accession numbers for the sequences reported in this paper are AF180968 and AF180969 for ITS-L and ITS-S, respectively.

et al. 2000

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PCR amplification of the internal transcribed spacer (ITS) between the 16SrRNA and 23S rRNA genes of the cyanobacterium Nostoc PCC 7120 gave three products. Two represented true ITS regions of different sizes, while the third was a heteroduplex. The longer spacer (ITS-L) contained 512 nucleotides and carried tRNA Ile and tRNA Ala genes, separated by a large stem-loop structure (V2) composed of short tandemly repeated repetitive sequences. Both tRNA genes, and the 5' half of the intervening stem, were absent from the shorter spacer (ITS-S), of length 283 nucleotides, which was otherwise almost completely identical to ITS-L. The two spacer regions of Nostoc PCC 7120 were aligned to published ITS sequences of cyanobacteria, the cyanelle of Cyanophora paradoxa and Escherichia coli. Although the ITS regions of cyanobacteria vary in length from 283 to 545 nucleotides and contain either both tRNA Ile and tRNA Ala genes, only the tRNA Ile gene, or neither, there is no correlation between ITS size and coding capacity for tRNAs. Putative secondary structures were determined for the deduced transcripts of the rrn operons of several cyanobacteria and were compared to that of E. coli. Highly conserved motifs important for folding and for maturation of the rRNA transcripts were identified, and regions homologous to bacterial antiterminators (box B-box A) were located. The conserved and variable regions of the cyanobacterial ITS are potential targets of PCR primers and oligonucleotide probes for detection and identification of cyanobacteria at different taxonomic levels.

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“…2) contained a perfect inverted repeat forming a 22 bp stem and a 4 b loop that might act as a transcription termination signal for petF1 as well as for the opposite str operon [25]. A putative ribosome binding site, complementary to the 3' end of cyanelle 16 S rRNA [27] was localized 12 bp upstream of the petF1 initiation codon. Sequence motifs resembling ' -10' and ' -35' promoter boxes were found on the 5' side of the Shine-Dalgarno sequence but more deviation from the prokaryotic consensus was observed than with cyanelle psbA and str promoters [19,25].…”

Section: Resultsmentioning

confidence: 99%

The petFI gene encoding ferredoxin I is located close to the str operon on the cyanelle genome of Cyanophora paradoxa

et al. 1990

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The petFI gene encoding ferredoxin I was localized in the large single copy region of cyanelle DNA by heterologous hybridization. Sequence analysis revealed an ORF of 99 amino acids (including the N-terminal processed methionine) at a position 477 bp from the 3' end of tufA but on the opposite strand. The 25 amino-terminal residues well corresponded to partial sequences obtained with purified cyanelle ferredoxin. The assignment of yet another gene that is not found on the genomes of chlorophyll b-type plastids to cyanelle DNA again corroborates the special position of cyanelles serving as a model for plastid evolution from endocytobiotic cyanobacteria.

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The central part of the cyanelle rDNA unit of Cyanophora paradoxa: Sequence comparison with chloroplasts and cyanobacteria

Cited by 31 publications

References 25 publications

The cyanelle str operon from Cyanophora paradoxa: Sequence analysis and phylogenetic implications

The cyanelle str operon from Cyanophora paradoxa: Sequence analysis and phylogenetic implications

Comparison of conserved structural and regulatory domains within divergent 16S rRNA–23S rRNA spacer sequences of cyanobacteria The GenBank accession numbers for the sequences reported in this paper are AF180968 and AF180969 for ITS-L and ITS-S, respectively.

The petFI gene encoding ferredoxin I is located close to the str operon on the cyanelle genome of Cyanophora paradoxa

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