The cellular uptake of meta-tetra(hydroxyphenyl)chlorin entrapped in organically modified silica nanoparticles is mediated by serum proteins

Compagnin, Chiara; Baù, Luca; Mognato, Maddalena; Celotti, Lucia; Miotto, Giovanni; Arduini, Maria; Moret, Francesca; Fede, Caterina; Secci, Francesco; Echevarria, I.M. Rio; Mancin, Fabrizio; Reddi, Elena

doi:10.1088/0957-4484/20/34/345101

Cited by 53 publications

(39 citation statements)

References 41 publications

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“…liposomal mTHPC formulations [28, 29] or the encapsulation of mTHPC into nanoparticles composed of poly(lactic-co-glycolic acid) [30], poly(lactic-co-glycolic acid)-b-poly(ethylene glycol) [31], poly(ethylene glycol) methacrylate-co-2-(diisopropylamino)ethyl methacrylate copolymers [32], human serum albumin [33], organic-modified silica [34] or calcium phosphate. [35] These studies describe promising carriers for mTHPC by improving solubility and reducing dark toxicity however it is not possible to directly compare them as very different model systems were used in each case.…”

Section: Discussionmentioning

confidence: 99%

Photoinduced effects of m-tetrahydroxyphenylchlorin loaded lipid nanoemulsions on multicellular tumor spheroids

et al. 2016

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BackgroundPhotosensitizers are used in photodynamic therapy (PDT) to destruct tumor cells, however, their limited solubility and specificity hampers routine use, which may be overcome by encapsulation. Several promising novel nanoparticulate drug carriers including liposomes, polymeric nanoparticles, metallic nanoparticles and lipid nanocomposites have been developed. However, many of them contain components that would not meet safety standards of regulatory bodies and due to difficulties of the manufacturing processes, reproducibility and scale up procedures these drugs may eventually not reach the clinics. Recently, we have designed a novel lipid nanostructured carrier, namely Lipidots, consisting of nontoxic and FDA approved ingredients as promising vehicle for the approved photosensitizer m-tetrahydroxyphenylchlorin (mTHPC).ResultsIn this study we tested Lipidots of two different sizes (50 and 120 nm) and assessed their photodynamic potential in 3-dimensional multicellular cancer spheroids. Microscopically, the intracellular accumulation kinetics of mTHPC were retarded after encapsulation. However, after activation mTHPC entrapped into 50 nm particles destroyed cancer spheroids as efficiently as the free drug. Cell death and gene expression studies provide evidence that encapsulation may lead to different cell killing modes in PDT.ConclusionsSince ATP viability assays showed that the carriers were nontoxic and that encapsulation reduced dark toxicity of mTHPC we conclude that our 50 nm photosensitizer carriers may be beneficial for clinical PDT applications.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-016-0221-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Photoinduced effects of m-tetrahydroxyphenylchlorin loaded lipid nanoemulsions on multicellular tumor spheroids

et al. 2016

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“…Recently, ORMOSIL nanoparticles with IR-820 for in vivo near-infrared fl uorescence imaging were successfully synthesized using the direct method [146] . In addition, ORMOSIL containing meta-tetra (hydroxyphenyl) chlorin [142] , silicon phthalocyanine 4 (Pc4) [144] , and protoporphyrin IX (PpIX) [146] were prepared for PDT.…”

Section: The Direct Methodsmentioning

confidence: 99%

“…ORMOSIL nanoparticles containing various photosensitizers such as HPPH [60,139,143] , 3-iodobenzyl-pyro [149] , silicon phthalocyanine 4 (Pc4) [144] , meta-tetra (hydroxyphenyl) chlorin [142] , and protoporphyrin IX (PpIX) [146] have been reported. Tumor cells effi ciently took up these nanoparticles containing photosensitizers in vitro , and then light irradiation of tumor cells results in substantial cell death.…”

Section: Photodynamic Therapy (Pdt)mentioning

confidence: 99%

Biomedical applications of organosilica nanoparticles toward theranostics

Nakamura

2012

Nanotechnology Reviews

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Nanoparticles for biomedical applications have several advantages as multifunctional agents. Among various types of nanoparticles for biomedical applications, silica nanoparticles have characteristic positioning due to their inherent property. The recent development of silica nanoparticles is creating a new trend in nanomedicine. A novel type of silica nanoparticle, organosilica nanoparticle, is both structurally and functionally different from the common (inorgano) silica nanoparticle. The organosilica nanoparticles are inherent organic-inorganic hybrid nanomaterials. The interior and exterior functionalities of organosilica nanoparticles are useful for their multifunctionalization. Biomedical applications of organosilica nanoparticles are leading to a wide range of nanomedical fi elds such as basic biomedical investigations and clinical applications. Multifunctionalizations peculiar to organosilica nanoparticles enable the creation of novel imaging systems and therapeutic applications. In this review, I will introduce differences between (inorgano)silica nanoparticles and organosilica nanoparticles, and then focus on biomedical applications of organosilica nanoparticles toward theranostics.

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“…As an alternative to pure silica, ORMOSIL NPs were investigated by the groups of Kopelmann and Prasad that encapsulated and successfully delivered to cells m-THPC [138], methylene blue [139] and 2-devinyl-2-(1-hexyloxyethyl)-pyropheophorbide (HPPH) [140]. Applying a modified Stöber procedure, which consists in using water and surfactants instead of organic solvents during synthesis, Mancin and coworkers produced naked and highly PEGylated ORMOSIL NPs with m-THPC physically entrapped in the silica matrix [141,142]. They reported that the presence of the PEG corona not only imparted stealth properties to the nanocarrier and stabilized the systems against aggregation, but also limited the transfer of m-THPC to serum proteins slowing down the rather fast PS release in cell culture medium therefore limiting protein-mediated cellular mTHPC uptake.…”

Section: Ceramic-based Npsmentioning

confidence: 99%

Strategies for optimizing the delivery to tumors of macrocyclic photosensitizers used in photodynamic therapy (PDT)

Moret

Reddi

2017

J. Porphyrins Phthalocyanines

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This review briefly summaries the principles and mechanisms of action of photodynamic therapy (PDT) as concerns its application in the oncological field, highlighting its drawbacks and some of the strategies that have been or are being explored to overcome them. The major aim is to increase the efficiency and selectivity of the photosensitizer (PS) uptake in the cancer cells for optimizing the PDT effects on tumors while sparing normal cells. Some attempts to achieve this are based on the conjugation of the PS to biomolecules (small ligands, peptides) functioning as carriers with the ability to efficiently penetrate cells and/or specifically recognize and bind proteins/receptors overexpressed on the surface of cancer cells. Alternatively, the PS can be entrapped in nanocarriers derived from various types of materials that can target the tumor by exploiting the enhanced permeability and retention (EPR) effects. The use of nanocarriers is particularly attractive because it allows the simultaneous delivery of more than one drug with the possibility of combining PDT with other therapeutic modalities.

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The cellular uptake of meta-tetra(hydroxyphenyl)chlorin entrapped in organically modified silica nanoparticles is mediated by serum proteins

Cited by 53 publications

References 41 publications

Photoinduced effects of m-tetrahydroxyphenylchlorin loaded lipid nanoemulsions on multicellular tumor spheroids

Photoinduced effects of m-tetrahydroxyphenylchlorin loaded lipid nanoemulsions on multicellular tumor spheroids

Biomedical applications of organosilica nanoparticles toward theranostics

Strategies for optimizing the delivery to tumors of macrocyclic photosensitizers used in photodynamic therapy (PDT)

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