The cellular dynamics of plant aquaporin expression and functions

Maurel, Christophe; Santoni, Véronique; Luu, Doan‐Trung; Wudick, Michael M.; Verdoucq, Lionel

doi:10.1016/j.pbi.2009.09.002

Cited by 135 publications

(97 citation statements)

References 75 publications

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“…Integrative (Ehlert et al, 2009) and cellular aspects (Van Wilder et al, 2008;Gattolin et al, 2009;Maurel et al, 2009) of plant-water relations and nutrition have been extensively investigated. However, the characteristics of plant aquaporins at the single-molecular level have remained unclear.…”

Section: Discussionmentioning

confidence: 99%

“…This process contributes to diverse responses, such as nutritional adjustments and modification of the number of active proteins at the plasma membrane (Takano et al, 2005). PIP molecules are subject to constitutive cycling between the plasma membrane and cytoplasm through processes of endo-and exocytosis (reviewed in Maurel et al, 2009). In addition, the alteration of PIP abundance at the plasma membrane through stimulus-induced trafficking of PIPs is a significant mechanism to regulate root water transport (Boursiac et al, 2005(Boursiac et al, , 2008Maurel et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…PIP molecules are subject to constitutive cycling between the plasma membrane and cytoplasm through processes of endo-and exocytosis (reviewed in Maurel et al, 2009). In addition, the alteration of PIP abundance at the plasma membrane through stimulus-induced trafficking of PIPs is a significant mechanism to regulate root water transport (Boursiac et al, 2005(Boursiac et al, , 2008Maurel et al, 2009). We observed a salt-induced intracellular accumulation of GFP-PIP2;1 and a reduced membrane density.…”

Section: Discussionmentioning

confidence: 99%

“…Although earlier studies focused on the structure and regulation mechanisms (Tö rnroth-Horsefield et al, 2006;Maurel et al, 2009), few have examined the diffusion dynamics and partitioning of membrane proteins in living cells, which is important for bridging data collected at the level of individual molecules to the physical and physiological processes (Joo et al, 2008). Bulk approaches have been unable to unambiguously determine the oligomeric state and mobility of molecules within living cells.…”

Section: Introductionmentioning

confidence: 99%

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Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation

et al. 2011

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PIP2;1 is an integral membrane protein that facilitates water transport across plasma membranes. To address the dynamics of Arabidopsis thaliana PIP2;1 at the single-molecule level as well as their role in PIP2;1 regulation, we tracked green fluorescent protein-PIP2;1 molecules by variable-angle evanescent wave microscopy and fluorescence correlation spectroscopy (FCS). Single-particle tracking analysis revealed that PIP2;1 presented four diffusion modes with large dispersion of diffusion coefficients, suggesting that partitioning and dynamics of PIP2;1 are heterogeneous and, more importantly, that PIP2;1 can move into or out of membrane microdomains. In response to salt stress, the diffusion coefficients and percentage of restricted diffusion increased, implying that PIP2;1 internalization was enhanced. This was further supported by the decrease in PIP2;1 density on plasma membranes by FCS. We additionally demonstrated that PIP2;1 internalization involves a combination of two pathways: a tyrphostin A23-sensitive clathrin-dependent pathway and a methyl-b-cyclodextrin-sensitive, membrane raft-associated pathway. The latter was efficiently stimulated under NaCl conditions. Taken together, our findings demonstrate that PIP2;1 molecules are heterogeneously distributed on the plasma membrane and that clathrin and membrane raft pathways cooperate to mediate the subcellular trafficking of PIP2;1, suggesting that the dynamic partitioning and recycling pathways might be involved in the multiple modes of regulating water permeability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation

et al. 2011

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“…It is now apparent that no matter the plant or the environment, control of water movements resides largely in the activity and specificity of aquaporins, especially those at the root-solution interfaces (Maurel et al 2008(Maurel et al , 2009). An exception may be the case of by-pass flow in rice (Yeo et al 1987;Faiyue et al 2010).…”

Section: Water and Transpirationmentioning

confidence: 99%

The integration of activity in saline environments: problems and perspectives

Cheeseman

2013

Functional Plant Biol.

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Abstract. The successful integration of activity in saline environments requires flexibility of responses at all levels, from genes to life cycles. Because plants are complex systems, there is no 'best' or 'optimal' solution and with respect to salt, glycophytes and halophytes are only the ends of a continuum of responses and possibilities. In this review, I briefly examine seven major aspects of plant function and their responses to salinity including transporters, secondary stresses, carbon acquisition and allocation, water and transpiration, growth and development, reproduction, and cytosolic function and 'integrity'. I conclude that new approaches are needed to move towards understanding either organismal integration or 'salt tolerance', especially cessation of protocols dependent on sudden, often lethal, shock treatments and the embracing of systems level resources. Some of the tools needed to understand the integration of activity and even 'salt stress' are already in hand, such as those for whole-transcriptome analysis. Others, ranging from discovery studies of the nature of the cytosol to expanded tool kits for proteomic, metabolomic and epigenomic studies, still need to be further developed. After resurrecting the distinction between applied stress and the resultant strain and noting that with respect to salinity, the strain is manifest in changes at all -omic levels, I conclude that it should be possible to model and quantify stress responses.

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