The Cellular and Molecular Patterns Involved in the Neural Differentiation of Adipose-Derived Stem Cells

Șelaru, Aida; Dinescu, Sorina; Costache, Marieta

doi:10.1007/5584_2020_547

Cited by 3 publications

(2 citation statements)

References 92 publications

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“…Adipose-derived stem cells (ADSCs), known to originate from stromalvascular fragments of adipose tissue, reportedly possess promising therapeutic potential [13]. Several studies have demonstrated that ADSCs can promote peripheral nerve regeneration [12,[14][15][16]. Furthermore, conditioned medium from ADSCs was found to enhance axonal regeneration [17,18].…”

Section: Characterization Of Isolated Exosomesmentioning

confidence: 99%

Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation

Rau

Kuo

et al. 2021

IJMS

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Exosomes secreted by adipose-derived stem cells (ADSC-exo) reportedly improve nerve regeneration after peripheral nerve injury. Herein, we investigated whether pretreatment of ADSCs with FK506, an immunosuppressive drug that enhances nerve regeneration, could secret exosomes (ADSC-F-exo) that further augment nerve regeneration. Designed exosomes were topically applied to injured nerve in a mouse model of sciatic nerve crush injury to assess the nerve regeneration efficacy. Outcomes were determined by histomorphometric analysis of semi-thin nerve sections stained with toluidine blue, mouse neurogenesis PCR array, and neurotrophin expression in distal nerve segments. Isobaric tags for relative and absolute quantitation (iTRAQ) were used to profile potential exosomal proteins facilitating nerve regeneration. We observed that locally applied ADSC-exo and ADSC-F-exo significantly enhanced nerve regeneration after nerve crush injury. Pretreatment of ADSCs with FK506 failed to produce exosomes possessing more potent molecules for enhanced nerve regeneration. Proteomic analysis revealed that of 192 exosomal proteins detected in both ADSC-exo and ADSC-F-exo, histone deacetylases (HDACs), amyloid-beta A4 protein (APP), and integrin beta-1 (ITGB1) might be involved in enhancing nerve regeneration.

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Section: Characterization Of Isolated Exosomesmentioning

confidence: 99%

Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation

Rau

Kuo

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Cell-based therapy is currently considered promising for the treatment of peripheral nerve injury [21,22]. Several studies have demonstrated that adipose-derived stem cells (ADSCs) [23][24][25] and even their conditioned medium can promote peripheral nerve regeneration [26,27]. The exosomes might be responsible for the effect of the secretome of the medium by transporting proteins, nucleic acids, and lipids into target cells for intercellular communication [28,29].…”

Section: Introductionmentioning

confidence: 99%

Exosomes Secreted by Adipose-Derived Stem Cells Following FK506 Stimulation Reduce Autophagy of Macrophages in Spine after Nerve Crush Injury

Kuo

Rau

et al. 2021

IJMS

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Macrophages emerge in the milieu around innervated neurons after nerve injuries. Following nerve injury, autophagy is induced in macrophages and affects the regulation of inflammatory responses. It is closely linked to neuroinflammation, while the immunosuppressive drug tacrolimus (FK506) enhances nerve regeneration following nerve crush injury and nerve allotransplantation with additional neuroprotective and neurotrophic functions. The combined use of FK506 and adipose-derived stem cells (ADSCs) was employed in cell therapy for organ transplantation and vascularized composite allotransplantation. This study aimed to investigate the topical application of exosomes secreted by ADSCs following FK506 treatment (ADSC-F-exo) to the injured nerve in a mouse model of sciatic nerve crush injury. Furthermore, isobaric tags for relative and absolute quantitation (iTRAQ) were used to profile the potential exosomal proteins involved in autophagy. Immunohistochemical analysis revealed that nerve crush injuries significantly induced autophagy in the dorsal root ganglia and dorsal horn of the spinal segments. Locally applied ADSC-F-exo significantly reduced autophagy of macrophages in the spinal segments after nerve crush injury. Proteomic analysis showed that of the 22 abundant exosomal proteins detected in ADSC-F-exo, heat shock protein family A member 8 (HSPA8) and eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) are involved in exosome-mediated autophagy reduction.

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Combining chitin biological conduits with injectable adipose tissue-derived decellularised matrix hydrogels loaded with adipose-derived mesenchymal stem cells for the repair of peripheral nerve defects in rats

Chen

Zhou

et al. 2023

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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The Cellular and Molecular Patterns Involved in the Neural Differentiation of Adipose-Derived Stem Cells

Cited by 3 publications

References 92 publications

Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation

Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation

Exosomes Secreted by Adipose-Derived Stem Cells Following FK506 Stimulation Reduce Autophagy of Macrophages in Spine after Nerve Crush Injury

Combining chitin biological conduits with injectable adipose tissue-derived decellularised matrix hydrogels loaded with adipose-derived mesenchymal stem cells for the repair of peripheral nerve defects in rats

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