The cellular activities of the subfraction of red onion peel crude ethanolic extract in MDA-MB-231 cells

Choe, Sin Pei; Siah, Poh Chiew; Lim, Shern Kwok; Peh, Kok Khiang; Ong, Ming Thong; Khoo, Boon Yin

doi:10.4103/pr.pr_20_20

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Quercetin content in subfraction F1 was detected by an ACQUITY UPLC system (Waters Corporation, Milford, MA, USA) incorporated with a photodiode array as the detector in the School of Biological Sciences, USM. F1 was prepared as described in Chao's previous study [ 14 ]. A 2.0 μ L aliquot of F1 or quercetin standard solution was injected into the UPLC BEH C18 column (100 mm × 2.1 mm i.d., 1.7 µ m particle size, Waters Corporation, Milford, Massachusetts, USA).…”

Section: Methodsmentioning

confidence: 99%

Comparative Studies on a Standardized Subfraction of Red Onion Peel Ethanolic Extract (Plant Substance), Quercetin (Pure Compound), and Their Cell Mechanism and Metabolism on MDA-MB-231

Leong

Chao

Siah

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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This study indicates the presence of quercetin in subfraction F1 and the standardized value of F1 derived from research using ultra-performance liquid chromatography (UPLC) and AlCl3 colorimetric assays, which further proved that both F1 and quercetin are potential growth inhibitors in MDA-MB-231 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In the process, staining of F1-treated cells with annexin/propidium iodide (PI) reduced cell proliferation and induced only S and G2 phases of cell cycle arrest in the treated cells by flow cytometry. Quercetin reduced cell proliferation by inducing apoptosis and S phase arrest. The 5′-bromo-2′-deoxyuridine (BrdU) incorporation of DNA synthesis in MDA-MB-231 cells was also inhibited after F1 and quercetin treatments. F1 and quercetin induced CYP1A1 and CYP1B1 gene expression, but only F1 induced CYP2S1 gene expression in the treated cells. Both F1 and quercetin inhibited the proliferation of MDA-MB-231 cells in different ways, but F1 is likely a better potential anticancer agent derived from the green approach towards breast cancer treatment.

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Section: Methodsmentioning

confidence: 99%

Comparative Studies on a Standardized Subfraction of Red Onion Peel Ethanolic Extract (Plant Substance), Quercetin (Pure Compound), and Their Cell Mechanism and Metabolism on MDA-MB-231

Leong

Chao

Siah

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Another study report revealed that the survival rate of HT-29 cells (human colon carcinoma cell line) was significantly reduced following 24 h incubation of cells with ethanol extract of yellow onion (Allium cepa L.) peel in a dose-dependent manner [31]. It has been demonstrated that a subfraction of the red onion (Allium cepa L.) peel ethanolic extract had a similar anticancerogenic activity against human breast cancer cells (MDA-MB-231) [32]. In agreement with the outcomes of the current study, Zhou et al (2020) demonstrated that extracts obtained from storey onion (Allium cepa L. var.…”

Section: Anticancer Effectsmentioning

confidence: 99%

Thermosonication Processing of Purple Onion Juice (Allium cepa L.): Anticancer, Antibacterial, Antihypertensive, and Antidiabetic Effects

Yıkmış,

Erdal,

Doguer

et al. 2024

Processes

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Onion (Allium cepa L.) juice is an important product used in gastronomy and food formulations. The first objective of this study was to optimize the content of bioactive compounds in purple onion juice (POJ) after the thermosonication process using response surface methodology (RSM) and artificial neural network (ANN) application models. Second, the anticancer, antibacterial, antihypertensive, and antidiabetic effects of POJ obtained after thermal pasteurization (P-POJ) or thermosonication (TS-POJ) were investigated after obtaining the ANN and RSM analysis reports. The optimization process for TS-POJ was carried out at 44 °C, for 13 min, with a 68% amplitude. The findings demonstrated that the angiotensin-converting enzyme (ACE) inhibition level was greater in TS-POJ samples than in the untreated control (C-POJ) sample (p > 0.05). C-POJ, TS-POJ, and P-POJ exhibited the inhibition of cell proliferation in vitro in a dose-dependent manner in lung (A549), cervical (HeLa), and colon cancer cells following 24 h incubation. Thermosonication or thermal pasteurization did not markedly affect the cell proliferation of the examined cancer cells compared to the untreated control group. While no antibacterial effect was observed with low concentrations of samples, they showed an antibacterial effect at pure concentrations (100%). The thermosonication treatment for processing purple onion juice was successful in this study’s results.

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“…Approximately 5 µl of the transformants with a dilution factor of 7.81×10 − 10 was then spotted in triplicate on the microbiological agar plates of YPD that contained 0 µg/ml (control), 25 µg/ml, 50 µg/ml, 75 µg/ml and 100 µg/ml quercetin (Cayman Chemical, Michigan, USA) or F1 [21]. The control contained < 1% of dimethyl sulfoxide (DMSO; Merck, Darmstadt, Germany).…”

Section: Screening Quercetin and F1 Effects By Yeast-based Assaymentioning

confidence: 99%

Quercetin and a plant substance, identified using a multi-copy number of DNA topoisomerase I in recombinant Pichia pastoris, inhibited MDA-MB-231 proliferation via different manners.

Zhao

Pei

Fadzil

et al. 2021

Preprint

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The study employed an in vivo strategy to construct a multi-copy number of human DNA topoisomerase I (hTopI) gene using pPIC3.5K vector in GS115 strain of Pichia. The yeast transformant (GS115-pPIC3.5K-hTopI; clone) was then used to investigate the preliminary growth effect of a pure compound (quercetin) and a standardised subfraction of ethanolic red onion peel extract (F1). The clones’ cell density was likely to be unaffected; only the total protein expression and enzyme activity were increased following the increased copy number of hTop1 in the host. The clone that showed the target enzyme's highest activity is said to respond specifically to growth inhibitors, whereby both quercetin and F1 were proven to be potential growth inhibitors as assessed by the MTT assay. In the process, quercetin reduced cell proliferation by inducing apoptosis and cell cycle arrest (S phase only), whereas F1 reduced cell proliferation by inducing cell cycle arrest only (S and G2 phases). Quercetin and F1 induced CYP1A1 and CYP1B1 (carcinogenicity) gene mRNA expression, but only F1 induced CYP2S1 (cytotoxicity) gene mRNA expression in the treated cells, suggesting that both quercetin and F1 inhibited the cell proliferation of MDA-MB-231 via different manners. The newly developed GS115-pPIC3.5K-hTopI can be used to select various potential substances for breast cancer treatment in the future.

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The cellular activities of the subfraction of red onion peel crude ethanolic extract in MDA-MB-231 cells

Cited by 5 publications

References 33 publications

Comparative Studies on a Standardized Subfraction of Red Onion Peel Ethanolic Extract (Plant Substance), Quercetin (Pure Compound), and Their Cell Mechanism and Metabolism on MDA-MB-231

Comparative Studies on a Standardized Subfraction of Red Onion Peel Ethanolic Extract (Plant Substance), Quercetin (Pure Compound), and Their Cell Mechanism and Metabolism on MDA-MB-231

Thermosonication Processing of Purple Onion Juice (Allium cepa L.): Anticancer, Antibacterial, Antihypertensive, and Antidiabetic Effects

Quercetin and a plant substance, identified using a multi-copy number of DNA topoisomerase I in recombinant Pichia pastoris, inhibited MDA-MB-231 proliferation via different manners.

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