The cells of the rabbit meniscus: their arrangement, interrelationship, morphological variations and cytoarchitecture

Graverand, Marie‐Pierre Hellio Le; Ou, Yongchung; SCHIELD-YEE, TERESA; Barclay, Leona; Hart, David A.; NATSUME, TAKASHI; Rattner, J. B.

doi:10.1017/s0021878201007671

Cited by 47 publications

(79 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have identifi ed four distinct cell morphologies in the meniscus, including cells in the inner, middle, outer regions and in the surface layer (Hellio Le Graverand et al, 2001). The inner region of the meniscus is generally described as more similar to cartilage, with rounded cells resembling articular chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of meniscal cell phenotypes using gene expression profiles

Son

Levenston²

2012

eCM

View full text Add to dashboard Cite

The lack of quantitative and objective metrics to assess cartilage and meniscus cell phenotypes contributes to the challenges in fi brocartilage tissue engineering. Although functional assessment of the fi nal resulting tissue is essential, initial characterization of cell sources and quantitative description of their progression towards the natural, desired cell phenotype would provide an effective tool in optimizing cell-based tissue engineering strategies. The purpose of this study was to identify quantifi able characteristics of meniscal cells and thereby fi nd phenotypical markers that could effectively categorize cells based on their tissue of origin (cartilage, inner, middle, and outer meniscus). The combination of gene expression ratios collagen VI/collagen II, ADAMTS-5/collagen II, and collagen I/collagen II was the most effective indicator of variation among different tissue regions. We additionally demonstrate a possible application of these quantifi able metrics in evaluating the use of serially passaged chondrocytes as a possible cell source in fi brocartilage engineering. Comparing the ratios of the passaged chondrocytes and the native meniscal cells may provide direction to optimize towards the desired cell phenotype. We have thus shown that measurable markers defi ning the characteristics of the native meniscus can establish a standard by which different tissue engineering strategies can be objectively assessed. Such metrics could additionally be useful in exploring the different stages of meniscal degradation in osteoarthritis and provide some insight in the disease progression.

show abstract

Section: Introductionmentioning

confidence: 99%

Discrimination of meniscal cell phenotypes using gene expression profiles

Son

Levenston²

2012

eCM

View full text Add to dashboard Cite

show abstract

“…2 Four morphologically distinct cell populations-stellate, branched, round, and fusiform-are present in rabbit menisci. 10 Moreover, human meniscus cells have several distinct populations: elongated fibroblast-like, polygonal, and rounded chondrocyte-like cells. 11 We previously demonstrated that human inner meniscus cells show ovoid and chondrocytic morphologies, although slender and fibroblastic cells are obtained from the outer halves of the menisci.…”

mentioning

confidence: 99%

Mechanical stretch enhances COL2A1 expression on chromatin by inducing SOX9 nuclear translocalization in inner meniscus cells

Kanazawa

Furumatsu

Hachioji

et al. 2011

Journal Orthopaedic Research

View full text Add to dashboard Cite

ABSTRACT:The meniscus plays an important role in controlling the biomechanics of the knee. However, the mechanical stress-related response in meniscus cells remains unclear. We investigated mechanical stretch-regulated gene expression in human meniscus cells. Human inner and outer meniscus cells were prepared from the inner and outer halves of the lateral meniscus. The gene expressions of Sry-type HMG box (SOX) 9 and a1(II) collagen (COL2A1) were assessed by real-time PCR analyses after cyclic tensile strain (CTS) treatment (0.5 Hz, 5% stretch). The localization and phosphorylation of SOX9 were evaluated by immunohistochemical and Western blot (WB) analyses. Chromatin immunoprecipitation (IP) analysis was performed to assess the stretch-related protein-DNA complex formation between SOX9 and the COL2A1 enhancer on chromatin. Type II collagen deposition and SOX9 production were detected only in inner menisci. CTS treatments increased expression of the COL2A1 and SOX9 genes in inner meniscus cells, but not in outer meniscus cells. In addition, CTS treatments stimulated nuclear translocalization and phosphorylation of SOX9 in inner meniscus cells. Chromatin IP analyses revealed that CTS increased the association between SOX9 and its DNA-binding site, included in the COL2A1 enhancer, on chromatin. Our results indicate that inner and outer meniscus cells have different properties in mechanical stretchinduced COL2A1 expression. In inner meniscus cells, mechanical stretch may have an essential role in the epigenetic regulation of COL2A1 expression. ß

show abstract

“…The meniscus contains 60-70% water and ϳ70% collagen by dry weight (4), as well as smaller amounts of proteoglycans, noncollagenous proteins, lipids, and cells (5). The extracellular matrix is maintained by meniscal fibrochondrocytes that exhibit phenotype characteristics of both fibroblasts and chondrocytes (6). The meniscus is an inhomogeneous structure that shows significant differences in composition, cellularity, and vascularity, depending on distance from the peripheral edge.…”

mentioning

confidence: 99%

“…The outer one-third of the meniscus (sometimes called the "red-red zone" because of its vascular supply) is composed of type I collagen (4,7,8). The cells in this region are fibroblast-like (6,9), and the tissue is vascularized by vessels that penetrate into the tissue from the perimeniscal capillary plexus (10). In contrast, the inner region of the meniscus (called the "white-white zone") contains fibrochondrocyte-like cells (6,9) as well as both type I and type II collagen (7,8), has a higher aggrecan content than the outer region, and is avascular (10).…”

mentioning

confidence: 99%

Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin‐1 or tumor necrosis factor α

McNulty

Moutos

Weinberg

et al. 2007

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To examine the hypotheses that increasing concentrations of interleukin-1 (IL-1) or tumor necrosis factor ␣ (TNF␣) inhibit the integrative repair of the knee meniscus in an in vitro model system, and that inhibitors of these cytokines will enhance repair.Methods. Explants (8 mm in diameter) were harvested from porcine medial menisci. To simulate a full-thickness defect, a 4-mm-diameter core was removed and reinserted. Explants were cultured for 14, 28, or 42 days in the presence of 0-1,000 pg/ml of IL-1 or TNF␣. Explants were also cultured in the presence of IL-1 or TNF␣ with IL-1 receptor antagonist (IL-1Ra) or TNF monoclonal antibody (mAb). At the end of the culture period, biomechanical testing, cell viability, and histologic analyses were performed to quantify the extent of repair.Results. Mechanical testing revealed increased repair strength, cell accumulation, and tissue formation at the interface over time under control conditions. Pathophysiologic concentrations of both IL-1 and TNF␣ significantly decreased repair strength, cell migration, and tissue formation at the interface. The addition of IL-1Ra or TNF mAb to explants prevented the effects of IL-1 or TNF␣, respectively. Conclusion. Our findings document that physiologically relevant concentrations of IL-1 and TNF␣ inhibit meniscal repair in vitro and therefore may also inhibit meniscal repair during arthritis or following joint injury. The finding that IL-1Ra and TNF mAb promoted integrative meniscal repair in an inflammatory microenvironment suggests that intraarticular delivery of IL-1Ra and/or TNF mAb may be useful clinically to promote meniscal healing following injury.

show abstract

The cells of the rabbit meniscus: their arrangement, interrelationship, morphological variations and cytoarchitecture

Cited by 47 publications

References 0 publications

Discrimination of meniscal cell phenotypes using gene expression profiles

Discrimination of meniscal cell phenotypes using gene expression profiles

Mechanical stretch enhances COL2A1 expression on chromatin by inducing SOX9 nuclear translocalization in inner meniscus cells

Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin‐1 or tumor necrosis factor α

Contact Info

Product

Resources

About