The cell-wall glycoproteins of the green alga Scenedesmus obliquus. The predominant cell-wall polypeptide of Scenedesmus obliquus is related to the cell-wall glycoprotein gp3 of Chlamydomonas reinhardtii

Voigt, J.; Stolarczyk, A.; Zych, Maria; Malec, Przemysław; Burczyk, J.

doi:10.1016/j.plantsci.2013.10.011

Cited by 50 publications

(38 citation statements)

References 58 publications

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“…1) proposed by Zhang et al [18] in which TAN is the total ammonia nitrogen in the reactor and temperature (T) was expressed in Kelvin. 8 [NH 3 ] = TAN 1+ 10 -pH 10 -(0.09018+2729.92/T)…”

Section: Methodsmentioning

confidence: 99%

“…Specifically, the genera Coprothermobacter is a thermophilic bacteria with high proteolytic activity able to degrade proteins into acetate, H2 and CO2 [52], and thus its presence is likely related to its ability to degrade components of the microalgal cell-wall. Scenedesmus have a high proportion of proteins and cellulose in their cells [8], and this would explain the high proportion of proteolytic and cellulolytic bacteria observed in the reactors. In contrast to the mesophilic AnMBR, the phylum Chloroflexi only accounted for 1.1% of all reads while the phylum Firmicutes is one of the dominant phyla in the thermophilic CSTR.…”

Section: Microbial Community In Thermophilic Cstrmentioning

confidence: 99%

“…However, the use of wastewater streams to cultivate microalgal biomass typically results in the growth of specific strains such as Scenedesmus [7], which possesses a rigid cell-wall. This cell-wall is composed of cellulose, hemicellulose and lignin [6,8] and is highly resistant to biological degradation [9].…”

Section: Introductionmentioning

confidence: 99%

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Microbial community characterization during anaerobic digestion of Scenedesmus spp. under mesophilic and thermophilic conditions

et al. 2017

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ElsevierGreses-Huerta, S.; Gaby, JC.; Aguado García, D.;Ferrer, J.; Seco Torrecillas, A.; Horn, SJ. (2017). Microbial community characterization during anaerobic digestion of Scenedesmus spp. under mesophilic and thermophilic conditions. AbstractMicrobial communities were thoroughly characterized in a mesophilic anaerobic membrane bioreactor (AnMBR) and a thermophilic continuous stirred tank reactor (CSTR), which were both treating recalcitrant microalgal biomass dominated by Scenedesmus. 16S rRNA amplicon sequencing analysis was performed when the AnMBR achieved 70% algal biodegradation and revealed high microbial diversity, probably due to the high solid retention time (SRT) of the AnMBR configuration. The bacterial community consisted of Chloroflexi (27.9%), WWE1 (19.0%) and Proteobacteria (15.4%) as the major phyla, followed by Spirochaetes (7.7%), Bacteroidetes 2 (5.9%) and Firmicutes (3.6%). These phyla are known to exhibit proteolytic and cellulolytic capabilities required to degrade the Scenedesmus cell-wall. Methanosaeta was the most abundant methanogen detected in the AnMBR suggesting that methane was mainly produced by the acetoclastic pathway. In comparison, the thermophilic CSTR achieved 32.6% algal biodegradation, and its bacterial community had fewer Operational Taxonomic Units (977 OTUs) than the AnMBR (1396 OTUs), as is generally observed for high temperature biogas reactors. However, phyla with high hydrolytic potential were detected such as Firmicutes (34.6%) and the candidate taxon EM3 (38.7%) in the thermophilic CSTR. Although the functional metabolism of EM3 in anaerobic digesters is unknown, the high abundance of EM3 suggests that this taxon plays an important role in the thermophilic, anaerobic degradation of Scenedesmus. The abundant syntrophic bacteria and the detection of hydrogenotrophic methanogens in the thermophilic CSTR suggest that the hydrogenotrophic pathway was the dominant pathway for methane production in this reactor.

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“…1) proposed by Zhang et al [18] in which TAN is the total ammonia nitrogen in the reactor and temperature (T) was expressed in Kelvin. 8 [NH 3 ] = TAN 1+ 10 -pH 10 -(0.09018+2729.92/T)…”

Section: Methodsmentioning

confidence: 99%

Section: Microbial Community In Thermophilic Cstrmentioning

confidence: 99%

See 1 more Smart Citation

Microbial community characterization during anaerobic digestion of Scenedesmus spp. under mesophilic and thermophilic conditions

et al. 2017

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“…This cell wall could be especially recalcitrant in microalgae species able to grow in stressing environments and used for wastewater remediation, such as Scenedesmus sp. or other coccoid green algae (Dunker and Wilhelm, 2018;Scholz et al, 2014;Voigt et al, 2014), given that it provides tolerance to high organic loads and physiological stresses (Gupta et al, 2016). In this regard, several mechanical treatments, most of them under alkaline conditions, have been tested.…”

Section: Introductionmentioning

confidence: 99%

Recovery of proteins from biomass grown in pig manure microalgae-based treatment plants by alkaline hydrolysis and acidic precipitation

Lorenzo-Hernando

Ruiz-Vegas

Vega

et al. 2019

Bioresource Technology

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The influence of three variables on key parameters of the protein extraction process (an alkaline hydrolysis followed by an acidic precipitation) for biomass from innovative photo-bioreactors for pig manure treatment was evaluated. Alkaline hydrolysis provided high solubilisation values (up to 66.5% of the biomass), augmenting with increasing values of the three studied variables (NaOH concentration, temperature and time). Nevertheless, moderate total (13.2%) and protein extraction yields (16.9%) were obtained, which was attributable to protein denaturation or to the low effectivity of the precipitation method. Extracts rich in proteins (53.5%-77.9%) with suitable amino acid profiles were obtained, but significant amounts of the initial lipids (up to 44.6%) were co-extracted probably due to fatty acids saponification. These results establish the first step for future studies in enhancing cell wall disruption and protein recovery by coupling alkaline hydrolysis with other mechanical pre-treatments, while considering alternative separation and purification methods. Highlights  Protein recovery by pH-shift from a consortium microalgae-bacteria biomass.  ANOVA of NaOH concentration, temperature and time effect on process parameters.  Intermediate alkaline concentration (0.5M NaOH) provided maxima extraction yields.  High solubilisation by alkaline hydrolysis but low acidic precipitation efficiency.  Co-extraction of lipids increased with process intensity.

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“…Scenedesmus sp. has a recalcitrant cell wall with a highly resistant trilaminar structure, composed of cellulose, algaenan and glucosamine-containing biopolymers and glycoproteins (Voigt et al, 2014). Spirulina has a much more easily degraded cell wall, composed of protein, polysaccharides and peptidoglycans (Van Eykelenburg, 1977).…”

Section: Introductionmentioning

confidence: 99%

Anaerobic digestion of Spirulina sp. and Scenedesmus sp.: a comparison and investigation of the impact of mechanical pre-treatment

et al. 2015

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Anaerobic digestion (AD) is a unit process that integrates beneficially and sustainably into many bioprocesses. This study assesses and compares the production of methane from the biomass of the microalga Scenedesmus sp. and the cyanobacterium Spirulina sp. in batch anaerobic digesters. Anaerobic digestion of whole cell Spirulina resulted in a substantially higher methane productivity (0.18 L CH4.Lreactor-1 .day-1) and methane yield (0.113 L CH4.g-1 volatile solids (VS)) compared to the digestion of whole cell Scenedesmus (0.12 L CH4.Lreactor-1 .day-1 and 0.054 L CH4.g VS-1). Spirulina, possibly due to a combination of osmotic shock, the filamentous nature of the cells and lower mechanical strength of the noncellulosic cell wall, was more readily degraded by hydrolytic and acidogenic microorganisms, resulting in the generation of a greater amount of acetic acid. This in turn provided greater substrate for methanogens and hence higher methane yields. In addition, Spirulina cells could be disrupted mechanically more quickly (1h) than Scenedesmus cells (4h) in a bead mill. Mechanical pre-treatment improved the final methane yields (L CH4.g VS-1) obtained from digestion of both substrates, however, the improvement was greater for Scenedesmus. Mechanical pre-treatment resulted in a 47% increase in methane production for Spirulina compared to 76% increase for Scenedesmus fed digesters. The more substantial increase observed for Scenedesmus was due to the relatively inefficient digestion of the whole, unruptured cells.

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The cell-wall glycoproteins of the green alga Scenedesmus obliquus. The predominant cell-wall polypeptide of Scenedesmus obliquus is related to the cell-wall glycoprotein gp3 of Chlamydomonas reinhardtii

Cited by 50 publications

References 58 publications

Microbial community characterization during anaerobic digestion of Scenedesmus spp. under mesophilic and thermophilic conditions

Microbial community characterization during anaerobic digestion of Scenedesmus spp. under mesophilic and thermophilic conditions

Recovery of proteins from biomass grown in pig manure microalgae-based treatment plants by alkaline hydrolysis and acidic precipitation

Anaerobic digestion of Spirulina sp. and Scenedesmus sp.: a comparison and investigation of the impact of mechanical pre-treatment

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