The Cell Wall-Associated Kinase (<i>WAK</i>) and<i>WAK</i>-Like Kinase Gene Family

Verica, Joseph A.; He, Zheng-Hui

doi:10.1104/pp.011028

Cited by 188 publications

(195 citation statements)

References 21 publications

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“…The structure of RFO1/WAKL22: RFO1 has been classified as WAKL22 on the basis of the backbone structure of its extracellular domain that is unique to the WAK/WAKL family (Verica and He 2002). The WAK/ WAKL family has been further divided into four subgroups.…”

Section: Discussionmentioning

confidence: 99%

RESISTANCE TO FUSARIUM OXYSPORUM 1, a Dominant Arabidopsis Disease-Resistance Gene, Is Not Race Specific

2005

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Arabidopsis thaliana ecotypes differ in their susceptibility to Fusarium wilt diseases. Ecotype Taynuilt-0 (Ty-0) is susceptible to Fusarium oxysporum forma specialis (f.) matthioli whereas Columbia-0 (Col-0) is resistant. Segregation analysis of a cross between Ty-0 and Col-0 revealed six dominant RESISTANCE TO FUSARIUM OXYSPORUM (RFO) loci that significantly contribute to f. matthioli resistance in Col-0 relative to Ty-0. We refer to the locus with the strongest effect as RFO1. Ty-0 plants in which only the Col-0 allele of RFO1 (RFO1 Col-0 ) was introduced were resistant to f. matthioli. Surprisingly, RFO1Col-0 also conferred resistance to f. raphani, demonstrating that RFO1-mediated resistance is not race specific. Expression of resistance by RFO2, RFO4, or RFO6 was dependent on RFO1 . Map-based cloning of RFO1 showed that RFO1 is identical to the previously named Arabidopsis gene WAKL22 (WALL-ASSOCIATED KINASE-LIKE KINASE 22), which encodes a receptor-like kinase that does not contain an extracellular leucine-rich repeat domain. Consistent with these results, a Col-0 rfo1 loss-of-function mutant was more susceptible to f. matthioli, f. conglutinans, and f. raphani. Thus, RFO1 encodes a novel type of dominant disease-resistance protein that confers resistance to a broad spectrum of Fusarium races.

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Section: Discussionmentioning

confidence: 99%

RESISTANCE TO FUSARIUM OXYSPORUM 1, a Dominant Arabidopsis Disease-Resistance Gene, Is Not Race Specific

2005

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“…receptor-like kinases; Morillo and Tax, 2006) to cell wall modifications (e.g. WAK kinase; Verica and He, 2002). Because of their function as transcriptional activators or repressors of target genes, the transcription factors identified can be key regulators of various processes, from the regulation of metabolic activity (e.g.…”

Section: Developmental Regulations In Expanding Fruit Tissuesmentioning

confidence: 99%

Gene and Metabolite Regulatory Network Analysis of Early Developing Fruit Tissues Highlights New Candidate Genes for the Control of Tomato Fruit Composition and Development

et al. 2009

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Variations in early fruit development and composition may have major impacts on the taste and the overall quality of ripe tomato (Solanum lycopersicum) fruit. To get insights into the networks involved in these coordinated processes and to identify key regulatory genes, we explored the transcriptional and metabolic changes in expanding tomato fruit tissues using multivariate analysis and gene-metabolite correlation networks. To this end, we demonstrated and took advantage of the existence of clear structural and compositional differences between expanding mesocarp and locular tissue during fruit development (12-35 d postanthesis). Transcriptome and metabolome analyses were carried out with tomato microarrays and analytical methods including proton nuclear magnetic resonance and liquid chromatography-mass spectrometry, respectively. Pairwise comparisons of metabolite contents and gene expression profiles detected up to 37 direct gene-metabolite correlations involving regulatory genes (e.g. the correlations between glutamine, bZIP, and MYB transcription factors). Correlation network analyses revealed the existence of major hub genes correlated with 10 or more regulatory transcripts and embedded in a large regulatory network. This approach proved to be a valuable strategy for identifying specific subsets of genes implicated in key processes of fruit development and metabolism, which are therefore potential targets for genetic improvement of tomato fruit quality.

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“…The two-intron gene structure pattern of Arabidopsis WAK/WAKLs (Verica and He, 2002) was generally conserved in OsWAKs. However, OsWAKs with extra/ missing EGF-like domain, an extra/missing intron was observed in their extracellular regions.…”

Section: Wall-associated Kinase Gene Family-ricementioning

confidence: 99%

“…Alternatively, these sequences may encode nonfunctional WAKL isoforms. Bacterial expression of several WAKL members shows that they have auto-phosphorylation activity, and analysis of several of the WAKL proteins shows that they localize to the cell wall (Verica and He, 2002). This suggests that the WAKLs, like the WAKs, are protein kinases that are tightly associated with the cell wall.…”

Section: Wall-associated Kinase Like Genes-arabidopsismentioning

confidence: 99%