The Cell Nucleus: Biogenesis, Structure, and Function

Jackson, Dean A.

doi:10.1002/3527600906.mcb.200300097.pub2

Cited by 1 publication

(1 citation statement)

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“…We included tension contributions to the pressure in the NE only under hypoosmotic conditions, assuming the NE to be relaxed under hyperosmotic conditions. Certain volume fractions inside the cytosol and the nucleus were excluded from the effective solvent volumes undergoing changes osmolarity during the experiment 143,144 . We estimated that there are ~450 NPCs per cell (Fig.…”

Section: Mathematical Model Of Fluid Flow Across the Npc And Ne Durin...mentioning

confidence: 99%

Nuclear pores as conduits for fluid flow during osmotic stress

Hoffmann,

Kim,

Obarska-Kosinska

et al. 2024

Preprint

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Changing environmental conditions necessitate an immediate cellular adaptation to ensure survival.Dictyostelium discoideum, a bacteriovore slime mold present in the soil of most terrestrial ecosystems, is known for its ability to tolerate drastic changes in osmolarity. How the cells cope with the resulting mechanical stress remains understudied. Here we show thatD. discoideumhas extraordinarily elaborate and resilient nuclear pores that serve as conduits for massive fluid exchange between cytosol and nucleus. We capitalize on the unique properties ofD. discoideumcells to quantify flow across the nuclear envelope that is necessitated by changing nuclear size in response to osmotic stress. Based on mathematical concepts adapted from hydrodynamics, we conceptualize this phenomenon as porous flow across nuclear pores. This type of fluid flow is distinct from the canonically characterized modes of nucleocytoplasmic transport, i.e. passive diffusion and active nuclear transport, because of its dependence on pressure. Our insights are relevant in any biological condition that necessitates rapid nuclear size changes, which includes metastasizing cancer cells squeezing through constrictions, migrating cells and differentiating tissues.

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Section: Mathematical Model Of Fluid Flow Across the Npc And Ne Durin...mentioning

confidence: 99%