The cell cycle dependent phosphorylation of histone H3 is correlated with the condensation of plant mitotic chromosomes

Houben, Andreas; Wako, Tadayuki; Furushima-Shimogawara, Rieko; Presting, Gernot G.; Künzel, G.; Schubert, Ingo; Fukui, K.

doi:10.1046/j.1365-313x.1999.00496.x

Cited by 111 publications

(96 citation statements)

References 21 publications

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“…There are well-known histone codes for transcriptional silencing and activation and for condensation and decondensation of chromatin. In the case of transcriptional silencing, tri-methylation of K9 and/or K27 on histone H3 is essential (Lachner et al, 2003), and phosphorylation of S10 on this histone is associated with mitotic condensation (Houben et al, 1999). Our findings clearly demonstrate that the acetylation of H4 exhibits residue-, stage-, and chromosome region-specific dynamics during the cell cycle.…”

Section: Discussionmentioning

confidence: 55%

Comprehensive analysis of dynamics of histone H4 acetylation in mitotic barley cells

2005

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Nucleosomal histones are covalently modified at specific amino acid residues. In the case of histone H4, four lysines (K5, K8, K12, and K16) are acetylated. In the current studies, we examined the dynamics of histone H4 acetylation at K8 and K12 in mitotic barley cells using a three-dimensional immunofluorescent method. Based on the results and previous studies on the dynamics of K5 and K16 acetylation, we provide a comprehensive view of the dynamics of H4 acetylation. Interphase nuclei exhibit strong acetylation in the centromeric region at K5, K8 and K12. In the case of K12, strong acetylation at nucleolar organizing regions was observed from prophase to anaphase. The dynamics of K12 were closely related to those of K5. On the other hand, K8 exhibited a pattern of almost uniform acetylation from prophase to telophase and strong acetylation in distal regions of chromosomes at both metaphase and anaphase, which is very similar to the dynamics of K16 acetylation. Thus, it appears that there is pair-wise acetylation of K12 and K5 in the nucleolar organizing regions and of K8 and K16 in the gene-rich regions. Together, these results suggest that pair-wise dynamics of H4 acetylation regulate chromosomal structure and function during the cell cycle.

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Section: Discussionmentioning

confidence: 55%

Comprehensive analysis of dynamics of histone H4 acetylation in mitotic barley cells

2005

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“…A change in H3P distribution on barley chromosomes during mitosis, documented in LM studies (Houben et al, 1999;Manzanero et al, 2002), could be confirmed with indirect immunogold labeling and SEM analysis, revealing a correlation between increasing compaction of chromomeres and increasing signal intensity in the pericentric region. Up to prometaphase, at which stage the chromomeres are still loose and the centromeric constriction is not yet pronounced, H3P signals are evenly distributed on the chromosome (Fig.…”

Section: Chromosomes As a "Loaded Spring"mentioning

confidence: 67%

3D Analysis of chromosome architecture: advantages and limitations with SEM

Wanner

Schroeder-Reiter²,

Formanek³

2005

Cytogenet Genome Res

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Three-dimensional mitotic plant chromosome architecture can be investigated with the highest resolution with scanning electron microscopy compared to other microscopic techniques at present. Specific chromatin staining techniques making use of simultaneous detection of back-scattered electrons and secondary electrons have provided conclusive information on the distribution of DNA and protein in barley chromosomes through mitosis. Applied to investigate the structural effects of different preparative procedures, these techniques were the groundwork for the “dynamic matrix model” for chromosome condensation, which postulates an energy-dependent process of looping and bunching of chromatin coupled with attachment to a dynamic matrix of associated protein fibers. Data from SEM analysis shows basic higher order chromatin structures: chromomeres and matrix fibers. Visualization of nanogold-labeled phosphorylated histone H3 (ser10) with high resolution on chromomeres shows that functional modifications of chromatin can be located on structural elements in a 3D context.

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“…[79][80][81][82] Nonetheless, contradictory conclusions about the relationship between histone H3 phosphorylation and chromosome condensation were obtained in oocytes. Bui et al reported that the changes in histone H3 kinase activity accurately correspond with the phosphorylation of H3/Ser10 and chromosome condensation during porcine oocyte maturation, implicating that H3/Ser10ph is a key event in meiotic chromosome condensation.…”

Section: Functions Of Histone Modifications During Mammalian Oocyte Mmentioning

confidence: 99%

Histone modifications during mammalian oocyte maturation: Dynamics, regulation and functions

Gu¹,

Wang²,

Sun³

2010

Cell Cycle

124

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The cell cycle dependent phosphorylation of histone H3 is correlated with the condensation of plant mitotic chromosomes

Cited by 111 publications

References 21 publications

Comprehensive analysis of dynamics of histone H4 acetylation in mitotic barley cells

Comprehensive analysis of dynamics of histone H4 acetylation in mitotic barley cells

3D Analysis of chromosome architecture: advantages and limitations with SEM

Histone modifications during mammalian oocyte maturation: Dynamics, regulation and functions

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