1994
DOI: 10.1007/bf00209250
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The cell blot assay in analysis of rat anterior pituitary cell secretion

Abstract: We have investigated the efficacy of the cell blot assay in analysis of the secretion of hormones and peptides from rat anterior pituitary cells. The dissociated cells are cultured on pieces of translucent polyvinylidene difluoride membrane, on which their secretory products are adsorbed and subsequently immunostained. The area and integrated optical density of the stained 'halo' surrounding individual cells is measured by microscopical image processing and the values for basal secretion of a particular hormon… Show more

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Cited by 4 publications
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“…To examine the effect of ODN on SL release from goldfish pituitary cells, a cell immunoblot assay was carried out as described previously with modifications for use on isolated goldfish pituitary cells . This method allowed for semiquantitative measurement of SL release from individual cells .…”
Section: Methodsmentioning
confidence: 99%
“…To examine the effect of ODN on SL release from goldfish pituitary cells, a cell immunoblot assay was carried out as described previously with modifications for use on isolated goldfish pituitary cells . This method allowed for semiquantitative measurement of SL release from individual cells .…”
Section: Methodsmentioning
confidence: 99%