The cell biology of leukocyte-mediated proteolysis

Owen, Caroline A.; Campbell, Edward J.

doi:10.1002/jlb.65.2.137

Cited by 383 publications

(423 citation statements)

References 155 publications

(172 reference statements)

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“…After LPS infusion, the capacity of caseinolysis of PMN neutral proteases and acidic proteases has been shown by Moussaoui et al [50]. PMN are reported to contain acidic proteases such as cathepsins B, C, D or L [54]. These proteases are not at their optimal pH after degranulation since they are in the environment (milk) with a pH tending to neutrality, suggesting that neutral proteases cannot degrade proteins in an extra-cellular way.…”

Section: Caseinolysis By Pmnmentioning

confidence: 97%

“…During the inflammatory response, the activities of these proteases are significantly increased as reported for collagenase [60], elastase [5,60], cathepsin G only [5] and both cathepsins C and G [60]. The role of these proteases in the degradation of the extracellular matrix is well known [5,54]. Many studies report a high SCC during mastitis with mainly PMN [31].…”

Section: An Increase In Proteolytic Activitiesmentioning

confidence: 99%

“…[31,54]. After LPS infusion, the capacity of caseinolysis of PMN neutral proteases and acidic proteases has been shown by Moussaoui et al [50].…”

Section: Caseinolysis By Pmnmentioning

confidence: 99%

“…PMN proteases degrade other proteins of the extracellular matrix such as collagen, gelatins, laminin, etc. [54].…”

Section: The Relationship With the Increase Of The Epithelial Barriermentioning

confidence: 99%

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Polymorphonuclear proteolytic activity and milk composition change

Roux¹,

Laurent²,

Moussaoui³

2003

Vet. Res.

117

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-Relationships between Quarter Milk Cell Count (QMCC) and Tank Milk Cell Count (TMCC) with milk biochemical and technological parameters in milk and dairy products were investigated. All parameters measured were affected by the increase of TMCC and QMCC between 0 and 600 000 cells/mL. The variable effect of lactation stage which is different for different authors, is discussed. The three mechanisms, measured during the inflammation of the udder, implicated in the modification of milk quality are described (a decrease in synthesis, a decrease in the milk barrier permeability and an increase in proteolytic activities). The direct effect of plasmin in caseinolysis is well known; the specific role of the increase of somatic cells (especially PMN) in the modification of milk quality is described. Several specific proteolytic activities of PMN are described and the impact of these activities on caseinolysis is evaluated. Two hypothetical mechanisms of caseinolysis by PMN are suggested and a synthetic scheme of the role of plasmin, bacteria and somatic cells in caseinolysis is discussed. caseinolysis / mastitis / milk / PMN proteases / SCC

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Section: Caseinolysis By Pmnmentioning

confidence: 97%

Section: An Increase In Proteolytic Activitiesmentioning

confidence: 99%

“…[31,54]. After LPS infusion, the capacity of caseinolysis of PMN neutral proteases and acidic proteases has been shown by Moussaoui et al [50].…”

Section: Caseinolysis By Pmnmentioning

confidence: 99%

“…PMN proteases degrade other proteins of the extracellular matrix such as collagen, gelatins, laminin, etc. [54].…”

Section: The Relationship With the Increase Of The Epithelial Barriermentioning

confidence: 99%

See 2 more Smart Citations

Polymorphonuclear proteolytic activity and milk composition change

Roux¹,

Laurent²,

Moussaoui³

2003

Vet. Res.

117

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“…It has been suggested that the increased capillary permeability at inflammatory foci allows SSL to extravasate (14). Besides, the infiltration of inflammatory cells may actively or passively promote SSL localization, either by taking up liposomes in the circulation and subsequent infiltration into the inflamed tissue, or by facilitating liposome extravasation as a result of leukocyte-mediated tissue inury (16).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Schiffelers

Storm

Bakker-Woudenberg

2001

Pharmaceutical Research

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Purpose. To gain insight into the host factors influencing liposome localization at sites of bacterial infection. Methods. In a unilateral Klebsiella pneumoniae pneumonia rat model, capillary permeability and number of circulating leukocytes was quantified and related to the degree of liposome target localization. Results. Liposome localization was highest in the hemorrhagic zone of infection, a zone characterized by markedly increased capillary permeability and high bacterial numbers. Both liposome localization and capillary permeability correlated positively with severity of infection. Lung instillation of other inflammatory stimuli, such as lipopolysaccharide or 0.1 M HCl inducing increased capillary permeability, also promoted liposome localization. As liposomal target localization in leukopenic rats was similar to that in immunocompetent rats, contribution of circulating leukocytes seems limited. Intrapulmonary distribution of liposomes shows that leukocytes at the target site are involved in liposome uptake after extravasation. Conclusions. Increased capillary permeability plays a crucial role in liposome localization at the infected site, whereas contribution of leukocytes is limited. These results suggest inflammatory conditions that could benefit from liposomal drug delivery. The involvement of leukocytes in liposome uptake at the target site could be important information in the selection of appropriate drugs.

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