The cell barrier effect in rabbit corneas kept under eye bank conditions

Medin, Walther; Davanger, Martin

doi:10.1111/j.1755-3768.1989.tb04403.x

Acta Ophthalmologica

1989

DOI: 10.1111/j.1755-3768.1989.tb04403.x

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The cell barrier effect in rabbit corneas kept under eye bank conditions

Walther Medin

Martin Davanger

Abstract: The influence of endothelial cell pumping and of cell barrier functions on cultivated corneas has been studied by comparing the rate of weight increase in freeze-treated, ouabain-treated and fresh corneas. Inhibition of the endothelial cell pump activity increased the swelling rate from 0.5% of start weight per h (fresh corneas) to about 2% per h (ouabain-treated corneas), as measured after 24 h in culture medium. The cell barrier effect was estimated by comparing the swelling rate of ouabain-treated corneas a… Show more

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Cited by 6 publications

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“…This is the purpose of the present study. (2)) are taken from recent studies (Medin & Davanger 1988, 1989b; ei r absolute valuzhave not been published before.…”

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confidence: 99%

The swelling of cornea in bank cultivation. The role of barriers and peripheral edge

Medin

Davanger

1991

Acta Ophthalmologica

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The swelling of a rabbit cornea in organ culture is caused by influx of fluid via the surface. Data regarding the weight increase rates during the first 4 h of corneal buttons with different diameters, have been used to calculate the net influx per mm2 per h via the anterior + posterior surfaces on one hand, and via the peripheral edge on the other hand. In fresh corneas the contribution to the corneal swelling, of the net fluid uptake via the anterior + the posterior surfaces, is negligible. The influx via the peripheral edge is about the same in fresh and freeze treated buttons. For freeze treated corneas, the resistance for fluid transport via the anterior + posterior surfaces seems to be 4 times higher than the resistance for transport via the peripheral edge. This result indicates that the Descemet's membrane and the Bowman's layer may have some barrier effect. An alternative or supplementary explanation is that, at least in swollen corneas, there may be reduced resistance to fluid flow along the corneal plane as compared with the flow vertical to this plane.

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“…This is the purpose of the present study. (2)) are taken from recent studies (Medin & Davanger 1988, 1989b; ei r absolute valuzhave not been published before.…”

mentioning

confidence: 99%

The swelling of cornea in bank cultivation. The role of barriers and peripheral edge

Medin

Davanger

1991

Acta Ophthalmologica

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Assessment of the effects of cetypyridium chloride on water content of the collagen-keratocyte matrix of the mammalian corneal stroma ex vivo

Doughty

1999

Biochimica et Biophysica Acta (BBA) - General Subjects

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Influence of temperature on corneas stored in culture medium. A comparative study using functional and morphological methods

Sandboe

Medin

Frøslie

2003

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To investigate the influence of storage temperature on corneal swelling and on endothelial morphology in cultured corneas. Material and methods: Forty-eight rabbit corneas were separated into four groups of 12. The corneas were stored in culture medium at 37 (group 37), 34 (group 34), 31 (group 31) and 23 (room temperature) (group 23), respectively. All the corneas were monitored by weight recordings on days 0, 1, 2, 3, 4 and 7. On day 7, corneas were prepared for scanning electron microscopy and endothelial cell counts were performed. Results: Lowering the temperature of the culture medium resulted in less swelling. Both temperature and storage time had significant effects on corneal swelling (p < 0.001). On day 7, the observed mean weight increase was 131.2%, 143.0%, 172.5% and 199.7% in groups 23, 31, 34 and 37, respectively. The estimated mean daily weight increase for the corneas were 2.6%, 4.0%, 9.1% and 16.0% in groups 23, 31, 34 and 37, respectively. Scanning electron microscopy showed an intact endothelial layer in all groups after 7 days and there were no statistically significant differences in endothelial counts between groups 23, 31 and 34. In group 37, the cell borders were difficult to distinguish after 7 days and no meaningful count could be performed. Conclusions: The swelling rate of cultured corneas is significantly less at 23 and 31than it is at 34 and 37 during the first week. This is most likely the result of a greatly increased barrier effect at lower temperatures. Whereas weight recording revealed profound differences between the groups, scanning electron microscopy and endothelial cell counting did not. The results support the hypothesis that storage at 37is not optimal in culturing corneas. Lowering the temperature below body temperature, and even lower than 31, results in less corneal swelling.

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The cell barrier effect in rabbit corneas kept under eye bank conditions

Cited by 6 publications

References 6 publications

The swelling of cornea in bank cultivation. The role of barriers and peripheral edge

The swelling of cornea in bank cultivation. The role of barriers and peripheral edge

Assessment of the effects of cetypyridium chloride on water content of the collagen-keratocyte matrix of the mammalian corneal stroma ex vivo

Influence of temperature on corneas stored in culture medium. A comparative study using functional and morphological methods

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