The cDNA Sequencing Project

Urushihara, Hideko; Morio, Takahiro; Tanaka, Yoshimasa

doi:10.1385/1-59745-144-4:31

Cited by 17 publications

(15 citation statements)

References 24 publications

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“…The authors thank S. Mitchell for Dictyostelium SBDS protein purification, the Japanese cDNA project 36 for providing clone VHA344, dictyBase for expression plasmids, N. Perrimon for plasmid pS5⌬H-G4MINT, and J. Rommens for lymphoblastoid cell lines. They thank F. Begum, S. Peak-Chew, and E. Stephens for mass spectrometry, as well as members of A.J.W.…”

Section: Acknowledgmentsmentioning

confidence: 99%

Defective ribosome assembly in Shwachman-Diamond syndrome

Wong

Traynor

Basse

et al. 2011

Blood

143

146

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Shwachman-Diamond syndrome (SDS), a recessive leukemia predisposition disorder characterized by bone marrow failure, exocrine pancreatic insufficiency, skeletal abnormalities and poor growth, is caused by mutations in the highly conserved SBDS gene. Here, we test the hypothesis that defective ribosome biogenesis underlies the pathogenesis of SDS. We create conditional mutants in the essential SBDS ortholog of the ancient eukaryote Dictyostelium discoideum using temperature-sensitive, selfsplicing inteins, showing that mutant cells fail to grow at the restrictive temperature because ribosomal subunit joining is markedly impaired. Remarkably, wild type human SBDS complements the growth and ribosome assembly defects in mutant Dictyostelium cells, but diseaseassociated human SBDS variants are defective. SBDS directly interacts with the GTPase elongation factor-like 1 (EFL1) on nascent 60S subunits in vivo and together they catalyze eviction of the ribosome antiassociation factor eukaryotic initiation factor 6 (eIF6), a prerequisite for the translational activation of ribosomes. Importantly, lymphoblasts from SDS patients harbor a striking defect in ribosomal subunit joining whose magnitude is inversely proportional to the level of SBDS protein.

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Section: Acknowledgmentsmentioning

confidence: 99%

Defective ribosome assembly in Shwachman-Diamond syndrome

Wong

Traynor

Basse

et al. 2011

Blood

143

146

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“…The genome sequence is maintained and annotated at dictyBase (http://dictybase.org/), which also runs the Dictyostelium Stock Center. Also, a largescale EST project has generated cDNAs representing 55% of the estimated genes, and many cDNAs are available as full-length inserts (Urushihara et al 2006).…”

Section: Dictyostelium Genomics and Molecular Geneticsmentioning

confidence: 99%

Dictyostelium Finds New Roles to Model

Williams

2010

Genetics

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Any established or aspiring model organism must justify itself using two criteria: does the model organism offer experimental advantages not offered by competing systems? And will any discoveries made using the model be of wider relevance? This review addresses these issues for the social amoeba Dictyostelium and highlights some of the organisms more recent applications. These cover a remarkably wide gamut, ranging from sociobiological to medical research with much else in between.

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“…The amtA DNA sequence was determined by sequencing cDNA clones SSB490, SSB126, and SLB420, generated by the Japanese cDNA project (51,67,68), and the corresponding region of genomic DNA.…”

Section: Strainsmentioning

confidence: 99%

Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum

et al. 2007

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Ammonia has been shown to function as a morphogen at multiple steps during the development of the cellular slime mold Dictyostelium discoideum; however, it is largely unknown how intracellular ammonia levels are controlled. In the Dictyostelium genome, there are five genes that encode putative ammonium transporters: amtA, amtB, amtC, rhgA, and rhgB. Here, we show that AmtA regulates ammonia homeostasis during growth and development. We found that cells lacking amtA had increased levels of ammonia/ammonium, whereas their extracellular ammonia/ ammonium levels were highly decreased. These results suggest that AmtA mediates the excretion of ammonium. In support of a role for AmtA in ammonia homeostasis, AmtA mRNA is expressed throughout the life cycle, and its expression level increases during development. Importantly, AmtA-mediated ammonia homeostasis is critical for many developmental processes. amtA ؊ cells are more sensitive to NH 4 Cl than wild-type cells in inhibition of chemotaxis toward cyclic AMP and of formation of multicellular aggregates. Furthermore, even in the absence of exogenously added ammonia, we found that amtA ؊ cells produced many small fruiting bodies and that the viability and germination of amtA ؊ spores were dramatically compromised. Taken together, our data clearly demonstrate that AmtA regulates ammonia homeostasis and plays important roles in multiple developmental processes in Dictyostelium.

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The cDNA Sequencing Project

Cited by 17 publications

References 24 publications

Defective ribosome assembly in Shwachman-Diamond syndrome

Defective ribosome assembly in Shwachman-Diamond syndrome

Dictyostelium Finds New Roles to Model

Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum

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