The cDNA cloning and mRNA expression of heat shock protein 70 gene in the haemocytes of bay scallop (Argopecten irradians, Lamarck 1819) responding to bacteria challenge and naphthalin stress

Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge

“…Deane et al [50] showed that the levels of Hsp70 were rapidly and drastically increased in sea bream (Salmonella sarba) exposed both to live V. alginolyticus and its extracellular product (ECP). Cellura et al [46] reported that pathogen V. anguillarum up-regulated Hsp70 gene expression from 12 to 48 h post-injection, and returned to baseline after 72 h. In the present study, the Hsp70 expression in haemocytes of P. fucata was up-regulated after V. alginolyticus challenge, and a clearly time-dependent expression pattern of PFHsp70 was observed, which is in good agreement with previous studies in other molluscs [27,34,46]. While the reason for the significant increase at 16 h post-injection was hard to explain.…”

“…As stress response proteins, heat shock proteins are supposed to act as a ''danger signal'' to immune cells, promote immune responses that are involved in protection of the cytoplasm components against not only various abiotic stressors, but also to biological stressors such as bacterial infections [20,27,47]. The Hsp70 family, one of the most abundant of various Hsps, has been confirmed maintaining cellular homeostasis and playing a role in protecting organism following pathogenic stress [7,21,22,[47][48].…”

“…In aquatic organisms, dramatic increases in the expression of Hsp70 mRNA in muscle and gill tissue were observed in pacific abalone (H. discus hannai) challenged with pathogenic bacteria Vibrio anguillarum [34]. Also, after injected bay scallop (A. irradians) by V. anguillarum, the mRNA expression of Hsp70 in the haemocytes reached a maximum level at 8 h and lasted to 16 h [27]. Deane et al [50] showed that the levels of Hsp70 were rapidly and drastically increased in sea bream (Salmonella sarba) exposed both to live V. alginolyticus and its extracellular product (ECP).…”

“…Several cDNAs encoding Hsp70s have been described in molluscs, including Crassostrea gigas [23], Ostrea edulis [24], Mytilus edulis [25], Chlamys farreri [26], and Argopecten irradians [27], Also, recent studies examining the effects of environmental stressors, including seasonal changes [28,29], salinity [30], PCBs [31], PAHs [32], heavy metal accumulation [33], and bacterial infections [27,34], on Hsp70 gene expression have been reported in different species of molluscs [19], and recognized the relevant physiological and ecological importance of heat shock gene expression in response of changing environments [9,14,15,[35][36][37]. However, no information is available on Hsp70 expression of Pinctada fucata at transcriptional level under bacterial challenge.…”

“…The mortalities resulted in a severe curtailment in maricultured pearls and the destruction of available pearl oyster resources. Although the causes for the oyster mortalities are still unclear, it is believed that the mortalities are related to a complicated interaction of multiple factors, such as pathogens, deteriorating water quality and stock degeneration from inbreeding [27].…”