The CD271 expression could be alone for establisher phenotypic marker in Bone Marrow derived mesenchymal stem cells.

Flores-Torales, Edgardo; Orozco-Barocio, Arturo; González-Ramella, Óscar; Carrasco-Yalán, Antonio A.; Gazarian, Karlen; Cuneo-Pareto, Silvio

doi:10.2478/v10042-010-0063-6

Cited by 25 publications

(21 citation statements)

References 26 publications

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“…After 2 weeks of culture in an osteogenic medium, abundant matrix mineralization was seen in BM-CD271 but not in the CB-CD271 cells (n = 3; bar = 50 μm). cy of CB-CD271 cells was close to BM-CD271 cells in bone marrow, which was 0.53% [Flores-Torales et al, 2010]. Despite the similar CD271 cell frequency, numerous studies have confirmed a lower frequency of MSCs in umbilical cord blood than in bone marrow [Martins et al, 2009].…”

Section: Discussionmentioning

confidence: 61%

“…In addition, the time from the collection of umbilical cord blood to isolation, its volume and content of mononuclear cells all contribute to the yield of MSC isolation [Bieback et al, 2004;Sibov et al, 2012]. Flores-Torales et al [2010] found that CD271+ cell population in bone marrow was highly correlated with the one defined by a group of stem cell markers. In the present study, BM-CD271 and CB-CD271 cells shared certain cell surface markers and differed in others.…”

Section: Discussionmentioning

confidence: 99%

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CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Watson

Foo

et al. 2013

Cells Tissues Organs

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CD271 has been applied to isolate mesenchymal stem cells (MSCs) from bone marrow and other tissues. Umbilical cord blood is a unique resource of stem cells and endothelial progenitor cells. Isolation of MSCs from umbilical cord blood, however, has been inefficient and inconsistent. This study was designed to examine the potential application of CD271 as a marker for the isolation of MSCs from umbilical cord blood. CD271+ cells were isolated from umbilical cord blood and bone marrow using CD271 antibody-conjugated microbeads, and characterized in osteogenic, chondrogenic and adipogenic differentiation. CD271+ cells from umbilical cord blood were slow to proliferate compared with those isolated from bone marrow. While CD271+ cells from bone marrow differentiated into osteogenic, chondrogenic and adipogenic lineages, there were no sound indications of differentiation by CD271+ cells from umbilical cord blood under the same differentiation conditions applied to the CD271+ cells from bone marrow. The study also found that bone marrow CD271+ cells remarkably upregulated the expression of chondrogenic genes under chondrogenic differentiation induction. When implanted into bone defects in mice, CD271+ cells from bone marrow regenerated significant bone, but the counterparts in umbilical cord blood formed little bone in the bone defects. In conclusion, CD271 is an efficient marker for MSC isolation from bone marrow but has failed to isolate MSCs from umbilical cord blood. CD271+ cells in bone marrow are particularly chondrogenic. The property of CD271+ cells is unique but varies from different tissues.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Watson

Foo

et al. 2013

Cells Tissues Organs

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“…There is a lack of published information about the expression level of CD271 on ovine stem cells, but average CD271 expression on human bone marrow cells is reported to be 0.53% [Flores-Torales et al, 2010]. The expression of CD271 on bone marrow cells decreased after cultivation in vitro [Jones et al, 2006].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of CD271<sup>+</sup> Stem Cells Derived from Sheep Dermal Skin

Jahroomishirazi¹,

Bader²,

Ebert³

et al. 2014

Cells Tissues Organs

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Background: Mesenchymal stem cells (MSCs) have great promise in the field of regenerative medicine due to their differentiation potential into several lineages. Besides the bone marrow, MSCs can be obtained from the dermis, which represents a large stem cell reservoir in the skin. Sheep provide an appropriate large animal model for preclinical studies. In this study, we focused on the isolation and characterization of MSCs from sheep dermis as an alternative to bone marrow MSCs (bmMSCs). Methods: Primary ovine cells were obtained from the dermis for comparison with bone marrow. CD271⁺/45^- dermal MSCs (CD271-dMSCs), which were sorted by flow cytometry, and plastic-adherent bmMSCs were examined for morphology, proliferation and senescence-associated β-galactosidase activity in both low and high oxygen conditions. CD271 expression on cultured cells was assessed by flow cytometry. Adipogenic and osteogenic potentials of CD271-dMSCs were evaluated by oil red O and von Kossa staining. Chondrogenic capacity of CD271-dMSCs and CD271⁺/CD45^- bone marrow cells (CD271-bmMSCs) was detected using immunohistochemistry and measurement of sulfated glycosaminoglycans. Results: The cell proliferation assay demonstrated no significant difference between CD271-dMSCs and bmMSCs under low oxygen conditions. Cultured CD271-dMSCs revealed much more CD271 expression compared to CD271-bmMSCs. CD271-dMSCs and CD271-bmMSCs showed basically similar expression of the cartilage-specific proteins aggrecan and collagen type II, although with a stronger staining in CD271-bmMSC-derived cultures. Remarkably, there was co-expression of CD271 and aggrecan during chondrogenic differentiation, suggesting an involvement of CD271 in chondrogenesis. Conclusion: Based on these findings, CD271-dMSCs might serve as an appropriate alternative cell source in preclinical research.

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“…In fact, one of the criteria for defining bone marrow stromal cells proposed by the International Society for Cellular Therapy is the expression of CD105, CD90, and CD73 and lack of CD45, CD34, or CD14 cell surface markers [35]. More recently CD146 and CD271 were suggested as specific MSC markers [36,37]. Interestingly, our data show little alteration in the expression of the MSC surface markers CD44 and CD90 in long-term culture, despite the reduction in stemness properties of the cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of Medium Supplements on Proliferation, Differentiation Potential, and In Vitro Expansion of Mesenchymal Stem Cells

Gharibi

Hughes

2012

Stem Cells Translational Medicine

174

152

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Mesenchymal stem cells (MSCs) possess great potential for use in regenerative medicine. However, their clinical application may be limited by the ability to expand their cell numbers in vitro while maintaining their differential potentials and stem cell properties. Thus the aim of this study was to test the effect of a range of medium supplements on MSC self-renewal and differentiation potential. Cells were cultured until confluent and subcultured continuously until reaching senescence. Medium supplementation with fibroblast growth factor (FGF)-2, platelet-derived growth factor (PDGF)-BB, ascorbic acid (AA), and epidermal growth factor (EGF) both increased proliferation rate and markedly increased number of cell doublings before reaching senescence, with a greater than 1,000-fold increase in total cell numbers for AA, FGF-2, and PDGF-BB compared with control cultures. Long-term culture was associated with loss of osteogenic/adipocytic differentiation potential, particularly with FGF-2 supplementation but also with AA, EGF, and PDGF-BB. In addition FGF-2 resulted in reduction in expression of CD146 and alkaline phosphatase, but this was partially reversible on removal of the supplement. Cells expressed surface markers including CD146, CD105, CD44, CD90, and CD71 by flow cytometry throughout, and expression of these putative stem cell markers persisted even after loss of differentiation potentials. Overall, medium supplementation with FGF-2, AA, EGF, and PDGF-BB greatly enhanced the total in vitro expansion capacity of MSC cultures, although differentiation potentials were lost prior to reaching senescence. Loss of differentiation potential was not reflected by changes in stem cell surface marker expression. STEM CELLS TRANSLATIONAL MEDICINE 2012;1:771-782

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The CD271 expression could be alone for establisher phenotypic marker in Bone Marrow derived mesenchymal stem cells.

Cited by 25 publications

References 26 publications

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Isolation and Characterization of CD271<sup>+</sup> Stem Cells Derived from Sheep Dermal Skin

Effects of Medium Supplements on Proliferation, Differentiation Potential, and In Vitro Expansion of Mesenchymal Stem Cells

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