The Ccr4-Not complex monitors the translating ribosome for codon optimality

Buschauer, Robert; Matsuo, Yoshitaka; Chen, Ying-Hsin; Alhusaini, Najwa; Sweet, Thomas J.; Sugiyama, Takato; Ikeuchi, Ken; Cheng, Jingdong; Matsuki, Yasuko; Gilmozzi, Andrea; Berninghausen, Otto; Becker, Thomas; Coller, Jeff; Inada, Toshifumi; Beckmann, Roland

doi:10.1101/854810

Cited by 21 publications

(43 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In other organisms NOT4 contributes to post-transcriptional control as a component of the CCR4-NOT complex, central to mRNA 3′ deadenylation, promotion of decapping and subsequent mRNA decay 25,26 . NOT4 in yeast was recently shown to be essential for the turnover of mRNAs with low codon optimality during translation, triggering recruitment of the CCR4-NOT complex through catalysing ribosome ubiquitination 61 . Although the presence of NOT4 within the CCR4-NOT complex in plants has yet to be established 62 , a recent proteomic characterisation of Target of rapamycin (TOR) signalling in Arabidopsis identified NOT4A as a TOR-regulated phosphoprotein, and NOT4B as an LST8 interactor along with other conserved CCR4-NOT core subunits, suggesting the plant NOT4s may indeed be subunits within this complex 63 .…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE:The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation

et al. 2021

View full text Add to dashboard Cite

Chloroplast function requires the coordinated action of nuclear- and chloroplast-derived proteins, including several hundred nuclear-encoded pentatricopeptide repeat (PPR) proteins that regulate plastid mRNA metabolism. Despite their large number and importance, regulatory mechanisms controlling PPR expression are poorly understood. Here we show that the Arabidopsis NOT4A ubiquitin-ligase positively regulates the expression of PROTON GRADIENT REGULATION 3 (PGR3), a PPR protein required for translating several thylakoid-localised photosynthetic components and ribosome subunits within chloroplasts. Loss of NOT4A function leads to a strong depletion of cytochrome b6f and NAD(P)H dehydrogenase (NDH) complexes, as well as plastid 30 S ribosomes, which reduces mRNA translation and photosynthetic capacity, causing pale-yellow and slow-growth phenotypes. Quantitative transcriptome and proteome analysis of the not4a mutant reveal it lacks PGR3 expression, and that its molecular defects resemble those of a pgr3 mutant. Furthermore, we show that normal plastid function is restored to not4a through transgenic PGR3 expression. Our work identifies NOT4A as crucial for ensuring robust photosynthetic function during development and stress-response, through promoting PGR3 production and chloroplast translation.

show abstract

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE:The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The atomic models PDB 5NDG (Prokhorova et al 2017), 6FYY, 6FYX (Llacer et al , 2018), and 6TB3 (Buschauer et al 2020) containing the models for S . c .…”

Section: Methodsmentioning

confidence: 99%

A structural inventory of native ribosomal ABCE1‐43S pre‐initiation complexes

et al. 2020

Self Cite

View full text Add to dashboard Cite

In eukaryotic translation, termination and ribosome recycling phases are linked to subsequent initiation of a new round of translation by persistence of several factors at ribosomal sub‐complexes. These comprise/include the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP‐binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of the next 43S pre‐initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1‐containing pre‐initiation complexes by cryo‐EM. We found that ABCE1 was mostly associated with early 43S, but also with later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide‐binding domains, while interacting with the N‐terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C‐terminus providing a structural explanation for its antagonistic role with respect to mRNA binding. Overall, the native human samples provide a near‐complete molecular picture of the architecture and sophisticated interaction network of the 43S‐bound eIF3 complex and the eIF2 ternary complex containing the initiator tRNA.

show abstract

“…First, the Not5 subunit interacts with Rpb4 and is required for its cytoplasmic translationrelated functions (Villanyi et al, 2014). Second, CCR4-Not promotes the degradation of inefficiently translated mRNAs by monitoring decoding rates (Webster et al, 2018;Buschauer et al, 2020). Third, together with the eIF4A2 protein, it may participate in the miRNAmediated inhibition of translation initiation of certain transcripts possessing purine-rich sequences within their 5 0 UTR (Wilczynska et al, 2019).…”

Section: Protein Factors Regulating Both Transcription and Translationmentioning

confidence: 99%

So close, no matter how far: multiple paths connecting transcription to mRNA translation in eukaryotes

Slobodin

Dikstein

2020

EMBO Reports

View full text Add to dashboard Cite

Transcription of DNA into mRNA and translation of mRNA into proteins are two major processes underlying gene expression. Due to the distinct molecular mechanisms, timings, and locales of action, these processes are mainly considered to be independent. During the last two decades, however, multiple factors and elements were shown to coordinate transcription and translation, suggesting an intricate level of synchronization. This review discusses the molecular mechanisms that impact both processes in eukaryotic cells of different origins. The emerging global picture suggests evolutionarily conserved regulation and coordination between transcription and mRNA translation, indicating the importance of this phenomenon for the fine‐tuning of gene expression and the adjustment to constantly changing conditions.

show abstract

The Ccr4-Not complex monitors the translating ribosome for codon optimality

Cited by 21 publications

References 46 publications

RETRACTED ARTICLE:The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation

RETRACTED ARTICLE:The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation

A structural inventory of native ribosomal ABCE1‐43S pre‐initiation complexes

So close, no matter how far: multiple paths connecting transcription to mRNA translation in eukaryotes

Contact Info

Product

Resources

About