The CC-chemokine receptor 2 is involved in the control of ovarian folliculogenesis and fertility lifespan in mice

Santos, A. G. A.; Pereira, Luiza Aparecida Ansaloni Chagas; Viana, J. H. M.; Russo, Remo Castro; Campos-Júnior, Paulo Henrique Almeida

doi:10.1016/j.jri.2020.103174

Cited by 9 publications

(6 citation statements)

References 30 publications

(45 reference statements)

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“…In the FH group, the gene is over-represented in the CXCR chemokine receptor pathway. The chemokine receptors control ovarian folliculogenesis and fertility in mice [51]. The CXCRs have a potential role in follicular growth during adult life, potentially affecting the reproductive lifespan [51].…”

Section: Discussionmentioning

confidence: 99%

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

Banerjee

Diniz

Hollingsworth

et al. 2023

Genes

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Reproductive failure is a major contributor to inefficiency within the cow-calf industry. Particularly problematic is the inability to diagnose heifer reproductive issues prior to pregnancy diagnosis following their first breeding season. Therefore, we hypothesized that gene expression from the peripheral white blood cells at weaning could predict the future reproductive potential of beef heifers. To investigate this, the gene expression was measured using RNA-Seq in Angus–Simmental crossbred heifers sampled at weaning and retrospectively classified as fertile (FH, n = 8) or subfertile (SFH, n = 7) after pregnancy diagnosis. We identified 92 differentially expressed genes between the groups. Network co-expression analysis identified 14 and 52 hub targets. ENSBTAG00000052659, OLR1, TFF2, and NAIP were exclusive hubs to the FH group, while 42 hubs were exclusive to the SFH group. The differential connectivity between the networks of each group revealed a gain in connectivity due to the rewiring of major regulators in the SFH group. The exclusive hub targets from FH were over-represented for the CXCR chemokine receptor pathway and inflammasome complex, while for the SFH, they were over-represented for immune response and cytokine production pathways. These multiple interactions revealed novel targets and pathways predicting reproductive potential at an early stage of heifer development.

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Section: Discussionmentioning

confidence: 99%

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

Banerjee

Diniz

Hollingsworth

et al. 2023

Genes

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“…CCL2-CCR2 signaling initiates G protein-coupled receptor signaling in leukocytes, which includes the activation of PI3K/Akt/mTOR, a critical pathway also associated with follicular activation and survival ( 50 , 51 ). CCR2 plays a crucial role in the regulation of murine folliculogenesis ( 52 ). Antral follicle development and oocyte growth and maturation occur together; moreover, antral folliculogenesis affects oocyte development and maturation ( 53 ).…”

Section: Discussionmentioning

confidence: 99%

The effect of C–C motif chemokine ligand 2 supplementation on in vitro maturation of porcine cumulus-oocyte complexes and subsequent developmental competence after parthenogenetic activation

Kim

Choi

et al. 2023

Front. Vet. Sci.

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Porcine embryos are used for a variety of applications. However, the maturation rate in vitro remains low, and novel in vitro maturation (IVM) techniques that facilitate the collection of mature oocytes are necessary. C-C motif chemokine ligand 2 (CCL2) is a key periovulatory chemokine present in cumulus-oocyte complexes (COCs). We aimed to examine the effects of CCL2 supplementation during IVM on oocyte maturation and embryonic development. The CCL2 concentration was significantly higher in porcine follicular fluid (pFF) derived from follicles >8 mm in size than in pFF derived from smaller follicles. There was a significant increase in CCL2 mRNA levels in all follicular cells after IVM compared with that before IVM. We analyzed the localization of CCL2 and its receptor, the CCL2 receptor, in follicular cells. During IVM, different concentrations of CCL2 were added to COCs cultured in a maturation medium. After IVM, the group treated with 100 ng/mL CCL2 showed significantly higher metaphase II rates than the control group. All CCL2-treatment groups showed a significant increase in intracellular glutathione levels and a significant decrease in reactive oxygen species levels, compared to the control. In CCs treated with 100 ng/mL CCL2, the mRNA levels of BAX, CASP3, and NPR2 were significantly decreased. Furthermore, the mRNA levels of SOD1, SOD2, and CD44 were significantly increased. In oocytes treated with 10 ng/mL CCL2, mRNA levels of BAX and CASP3 were significantly decreased, whereas, NRF2 and NPM2 were significantly increased. ERK1 exhibited significantly increased mRNA expression in both CCs and oocytes treated with 10 ng/mL CCL2. The protein expression ratio of phosphorylated ERK1/2 to total ERK1/2 was significantly increased in CCs treated with 10 ng/mL CCL2. After parthenogenetic activation, cleavage rates were significantly improved in the 100 ng/mL CCL2 treatment group, and blastocyst formation rates were significantly enhanced in the 10 ng/mL CCL2 treatment group. Overall, our results suggest that IVM medium along with CCL2 improves porcine oocyte maturation and the development of parthenogenetically-activated embryos.

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“…Thereby controls ovarian function, fertilization, and ovulation. Its proinflammatory activity is linked to the formation of prostaglandin E by downregulation of COX-2 mRNA of granulosa cells whereas it is upregulated twice in PCOS [25]. It is also being proven that the PPAR-γ controls genes responsible for expression of TNFalpha and Interleukins along with others.…”

Section: Peroxisome Proliferator-activated Receptor Gammamentioning

confidence: 99%

“…Earlier study reported the expression of chemokine receptor-2 (C-C motif) (CCR2) in the human ovarian cumulus-oocyte complexes, theca cells, preovulatory follicles and in feline ovarian follicle walls. This receptor is believed to be involved in folliculogenesis and determines the reproductive lifespan of female [25] (Figure 1).…”

Section: Chemokinesmentioning

confidence: 99%

Inflammation and Ovulation

Pant¹,

Chitme²

2021

Fibroids

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The ovulation is a complex physiological process which is very commonly affected in patients with PCOS. Understanding inflammatory process involved in ovulation is important with respect to its onset, diagnosis and treatment. There are multiple inflammatory factors are associated with ovulation however anovulation and contraception have not been therapeutically explored in context with inflammatory process. Therefore, this chapter is written to help readers to understand the basics of inflammation in ovulation and role of inflammatory mediators in ovulation. This chapter also describes genetic and molecular aspects linked to ovulation.

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The CC-chemokine receptor 2 is involved in the control of ovarian folliculogenesis and fertility lifespan in mice

Cited by 9 publications

References 30 publications

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

The effect of C–C motif chemokine ligand 2 supplementation on in vitro maturation of porcine cumulus-oocyte complexes and subsequent developmental competence after parthenogenetic activation

Inflammation and Ovulation

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