The Caucasian Clover Gene TaMYC2 Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes

Zhao, Yihang; Yang, Yupeng; Jiang, Jingwen; Zhang, Xiaomeng; Ma, Zewang; Meng, Long; Cui, Guowen; Yin, Xiujie

doi:10.3390/genes13020329

Cited by 5 publications

(2 citation statements)

References 51 publications

(49 reference statements)

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“…Bieb.) by increasing antioxidant enzymes’ activities ( Zhao et al., 2022 ). It has also found that TaMYC2 overexpression significantly improved drought and cold tolerance of transgenic tobacco by increasing these activities.…”

Section: Discussionmentioning

confidence: 99%

Potential role of the regulatory miR1119-MYC2 module in wheat (Triticum aestivum L.) drought tolerance

Shamloo‐Dashtpagerdi¹,

Shahriari²,

Tahmasebi

et al. 2023

Front. Plant Sci.

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MicroRNA (miRNA)-target gene modules are essential components of plants’ abiotic stress signalling pathways Little is known about the drought-responsive miRNA-target modules in wheat, but systems biology approaches have enabled the prediction of these regulatory modules and systematic study of their roles in responses to abiotic stresses. Using such an approach, we sought miRNA-target module(s) that may be differentially expressed under drought and non-stressed conditions by mining Expressed Sequence Tag (EST) libraries of wheat roots and identified a strong candidate (miR1119-MYC2). We then assessed molecular and physiochemical differences between two wheat genotypes with contrasting drought tolerance in a controlled drought experiment and assessed possible relationships between their tolerance and evaluated traits. We found that the miR1119-MYC2 module significantly responds to drought stress in wheat roots. It is differentially expressed between the contrasting wheat genotypes and under drought versus non-stressed conditions. We also found significant associations between the module’s expression profiles and ABA hormone content, water relations, photosynthetic activities, H2O2 levels, plasma membrane damage, and antioxidant enzyme activities in wheat. Collectively, our results suggest that a regulatory module consisting of miR1119 and MYC2 may play an important role in wheat’s drought tolerance.

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Section: Discussionmentioning

confidence: 99%

Potential role of the regulatory miR1119-MYC2 module in wheat (Triticum aestivum L.) drought tolerance

Shamloo‐Dashtpagerdi¹,

Shahriari²,

Tahmasebi

et al. 2023

Front. Plant Sci.

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“…These results indicate that TaeRF1 plays an active regulatory role in the responses of plants to LT stress. At the same time, TaMYC2 in Caucasian clover can also increase the activity of antioxidant enzymes in plants, and increase the expression of ROS scavenging related genes and stress response genes under LT and drought stress, thereby enhancing the response ability of transgenic plants to stress ( Zhao et al., 2022 ). Taken together, these findings provide further insights into the properties of TaeRF1 protein and how it protects plants at low temperatures, and provide references for clover breeding.…”

Section: Discussionmentioning

confidence: 99%

Cloning of TaeRF1 gene from Caucasian clover and its functional analysis responding to low-temperature stress

Zhang

Jiang

et al. 2022

Front. Plant Sci.

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Low temperature (LT) is an important threat to the normal growth of plants. In this study, based on the full-length transcriptome sequencing results, the cold resistance genes were cloned from Caucasian clover with strong cold resistance. We cloned the CDS of TaeRF1, which is 1311 bp in length and encodes 436 amino acids. The molecular weight of the protein is 48.97 kDa, which had no transmembrane structure, and its isoelectric point (pI) was 5.42. We predicted the structure of TaeRF1 and found 29 phosphorylation sites. Subcellular localization showed that TaeRF1 was localized and expressed in cell membrane and chloroplasts. The TaeRF1 gene was induced by stress due to cold, salt, alkali and drought and its expression level was higher in roots and it was more sensitive to LT. Analysis of transgenic A. thaliana plants before and after LT treatment showed that the TaeRF1 gene enhanced the removal of excess H2O2, and increased the activity of antioxidant enzymes, thus improving the plant’s ability to resist stress. Additionally, the OE lines showed increased cold tolerance by upregulating the transcription level of cold-responsive genes (CBF1, CBF2, COR15B, COR47, ICE1, and RD29A). This study demonstrates that TaeRF1 is actively involved in the responses of plants to LT stress. We also provide a theoretical basis for breeding and a potential mechanism underlying the responses of Caucasian clover to abiotic stress.

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VaMYB44 transcription factor from Chinese wild Vitis amurensis negatively regulates cold tolerance in transgenic Arabidopsis thaliana and V. vinifera

Zhang

et al. 2022

Plant Cell Rep

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The Caucasian Clover Gene TaMYC2 Responds to Abiotic Stress and Improves Tolerance by Increasing the Activity of Antioxidant Enzymes

Cited by 5 publications

References 51 publications

Potential role of the regulatory miR1119-MYC2 module in wheat (Triticum aestivum L.) drought tolerance

Potential role of the regulatory miR1119-MYC2 module in wheat (Triticum aestivum L.) drought tolerance

Cloning of TaeRF1 gene from Caucasian clover and its functional analysis responding to low-temperature stress

VaMYB44 transcription factor from Chinese wild Vitis amurensis negatively regulates cold tolerance in transgenic Arabidopsis thaliana and V. vinifera

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