The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)

Lissabet, Jorge Félix Beltrán; Belén, Lisandra Herrera; Lee‐Estévez, Manuel; Risopatrón, Jennie; Valdebenito, Iván; Figueroa, Elías; Farías, Jorge G.

doi:10.1016/j.cbpa.2019.110634

Cited by 16 publications

(17 citation statements)

References 62 publications

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“…However, we can speculate that changes in the sh sperm motility pattern before fertilization, and then probably even during the cooling process, may have something in common with the hyperactivated movement of mammalian spermatozoa which are about to fertilize eggs [38], or that occurs as a result of cryocapacitation [33,34]. Our speculation can be supported by recent ndings [39], in which the presence of cAMP-dependent protein kinase and CatSper-like protein in the spermatozoa of many sh, including trout, were identi ed. In particular, CatSper is a Ca 2+ -speci c channel of mammalian spermatozoa plasma membrane, which by mediating Ca 2 + i in ux induces the initiation of the vigorous and hyperactive sperm motility prior to fertilization [40,41].…”

Section: Discussionsupporting

confidence: 63%

Could the Storage Time Post-collection Affect the Post-thaw Motility and Fertilizing Ability of Mediterranean Brown Trout Semen?

Rusco

Iorio

Iampietro

et al. 2021

Preprint

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The aim of this study was to evaluate the effect of different cool storage time intervals between collection and semen freezing on both fresh and cryopreserved semen motility parameters and the post-thaw fertilizing ability of Mediterranean brown trout semen. The ejaculates were split into six aliquots and stored on ice for 1 to 6 hours, until freezing. Fresh and post-thawing sperm motility were evaluated by Computer-Assisted Sperm Analysis system, whilst the fertilizing ability was assessed by in vivo trials. In fresh semen, at 3 h of storage, a significant decrease of total motility, linear movement (STR, LIN) and beat cross frequency was recorded, whilst the amplitude of lateral displacement of the spermatozoon head underwent a significant increase. Velocity parameters (VCL, VAP and VSL) were not affected by the cold storage time, whilst the duration of sperm movement was significantly higher at 1h compared to the other times tested. Freezing procedure overall decreased almost all post-thaw sperm motility parameters, however no significant differences was observed over time, both in term of fast and linear movement. Cool storage time did not significantly affect the percentage of post-thaw eyed embryos. Our results showed that Mediterranean brown trout semen can be stored on ice even up to 6 hours before freezing, without decreasing its post-thawing quality and fertilizing ability.

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Section: Discussionsupporting

confidence: 63%

Could the Storage Time Post-collection Affect the Post-thaw Motility and Fertilizing Ability of Mediterranean Brown Trout Semen?

Rusco

Iorio

Iampietro

et al. 2021

Preprint

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“…According to the literature, homology of CatSper among species is also low [ 64 ]. Following comparative genomics done by Cai and Clapham [ 65 ], genes of CatSperα and CatSperβ subunits have been lost through metazoan evolution, especially in vertebrate lineages, such as Agnatha, Teleostei, Amphibia, and Aves; however, that is in complete opposition to a study by Lissabet et al [ 66 ], where CatSper was found to be present in Salmo salar (the infraclass: Teleostei) sperm cells. Nevertheless, the CatSper channel has also been found in sea urchin [ 67 ] and a variety of mammalian species (human, mouse, horses, and boars) [ 58 , 68 , 69 , 70 , 71 ].…”

Section: Calcium Channelsmentioning

confidence: 99%

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Nowicka-Bauer

Szymczak-Cendlak

2021

IJMS

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Sperm motility is linked to the activation of signaling pathways that trigger movement. These pathways are mainly dependent on Ca2+, which acts as a secondary messenger. The maintenance of adequate Ca2+ concentrations is possible thanks to proper concentrations of other ions, such as K+ and Na+, among others, that modulate plasma membrane potential and the intracellular pH. Like in every cell, ion homeostasis in spermatozoa is ensured by a vast spectrum of ion channels supported by the work of ion pumps and transporters. To achieve success in fertilization, sperm ion channels have to be sensitive to various external and internal factors. This sensitivity is provided by specific channel structures. In addition, novel sperm-specific channels or isoforms have been found with compositions that increase the chance of fertilization. Notably, the most significant sperm ion channel is the cation channel of sperm (CatSper), which is a sperm-specific Ca2+ channel required for the hyperactivation of sperm motility. The role of other ion channels in the spermatozoa, such as voltage-gated Ca2+ channels (VGCCs), Ca2+-activated Cl-channels (CaCCs), SLO K+ channels or voltage-gated H+ channels (VGHCs), is to ensure the activation and modulation of CatSper. As the activation of sperm motility differs among metazoa, different ion channels may participate; however, knowledge regarding these channels is still scarce. In the present review, the roles and structures of the most important known ion channels are described in regard to regulation of sperm motility in animals.

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“…Several studies on different fish species such as rainbow trout [35][36][37], common carp [38], Atlantic croaker [39], and sterlet sturgeon [40] have reported that calcium chan-nels play a key role in regulating sperm motility. Recently, some authors have identified the presence of CatSper-like protein in the spermatozoa of many fish, including rainbow trout [41,42]. In particular, CatSper is a Ca 2+ -specific channel of mammalian spermatozoa plasma membrane, which by mediating Ca 2+ i influx induces the initiation of the vigorous and hyperactive sperm motility prior to fertilization [43,44].…”

Section: Discussionmentioning

confidence: 99%

Cryobank of Mediterranean Brown Trout Semen: Evaluation of the Use of Frozen Semen up to Six Hours Post-Collection

Rusco

Iorio

Iampietro

et al. 2021

Fishes

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The aim of this study was to evaluate the effects of different cold-storage time intervals between collection and semen-freezing on both fresh and cryopreserved semen motility parameters and the post-thaw fertilizing ability of Mediterranean brown trout semen. The ejaculates were split into six aliquots and stored on ice from 1 to 6 h, until freezing. Fresh and post-thaw sperm motility was evaluated by a Computer-Assisted Sperm Analysis system, whilst the fertilizing ability was assessed by in vivo trials. In fresh semen, at 3 h of storage, a significant decrease of total motility, linear movement (STR, LIN) and beat cross frequency (BCF) was recorded, whilst the amplitude of lateral displacement of the spermatozoon head (ALH) underwent a significant increase. In frozen semen, no significant difference was observed for all the motility parameters evaluated, except for the total motility between 1 and 6 h of storage and the duration of sperm movement between 1 and 5 h. Cold-storage time did not significantly affect the percentage of live embryos following the use of frozen semen. In conclusion, our results showed that, if necessary, the Mediterranean brown trout semen can be frozen even until 6 h post-collection without losing its fertilizing ability.

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The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar)

Cited by 16 publications

References 62 publications

Could the Storage Time Post-collection Affect the Post-thaw Motility and Fertilizing Ability of Mediterranean Brown Trout Semen?

Could the Storage Time Post-collection Affect the Post-thaw Motility and Fertilizing Ability of Mediterranean Brown Trout Semen?

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Cryobank of Mediterranean Brown Trout Semen: Evaluation of the Use of Frozen Semen up to Six Hours Post-Collection

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