The catalytic effect of molybdate on the hydrolysis of organic phosphate bonds

Weil‐Malherbe, H.; Green, R. H.

doi:10.1042/bj0490286

Cited by 380 publications

(85 citation statements)

References 10 publications

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“…Milk fractions including MFGM prepared from several samples of milk by this procedure were assayed for 5'-nucleotidase [4] , nucleotide pyrophosphatase [6] and ATPases [8] …”

Section: Methodsmentioning

confidence: 99%

The presence of plasma membrane enzymes on the surface of bovine milk fat globules

Patton

Trams

1971

FEBS Letters

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“…Milk fractions including MFGM prepared from several samples of milk by this procedure were assayed for 5'-nucleotidase [4] , nucleotide pyrophosphatase [6] and ATPases [8] …”

Section: Methodsmentioning

confidence: 99%

The presence of plasma membrane enzymes on the surface of bovine milk fat globules

Patton

Trams

1971

FEBS Letters

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“…PPi was determined colorimetrically in the presence of j3-mercaptoethanol and metabisulphite after three-fold extraction of orthophosphate in the from of a phosphomolebdate complex [ 16]. Inorganic orthophosphate was determined by the method of Behrenblum and Chain as modified by Weil-Malherbe and Green [ 17]. To identify PPi it was precipitated in the form of barium salts from the acid extract of mitochondria and subjected to t.l.c, on DEAE cellulose and paper.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of inorganic pyrophosphate by animal tissue mitochondria

et al. 1975

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“…The crude extracts were centrifuged at 2000g, and aliquots of the supernatant fractions were adjusted to 4 ml with water. Then 6 ml of isobutanol and 1 ml of acid molybdate reagent were added (24), and the method of Jennings and Morton was followed (11).…”

Section: Methodsmentioning

confidence: 99%

The Role of Phytic Acid in the Wheat Grain

Williams¹

1970

Plant Physiol.

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The concentrations of adenosine triphosphate and phytic acid in testa, embryo plus scutellum, aleurone, and endosperm fractions from grain of Triticum vulgare cv. Insignia have been determined during development under both normal conditions and those of water stress. Phytic acid was not detected in the endosperm. In the embryo plus scutellum and aleurone fractions there was a rapid build-up of phytic acid, but the adenosine triphosphate level did not change markedly at this time. These results are not consistent with physiological roles previously suggested for phytic acid other than the role of phytin as a phosphorus and cation store for the germinating seed.Phytic acid is a major phosphorus component of many seeds. In mature grain of Triticum vulgare cv. Gabo this compound, which accounts for about 88% of the acid-soluble phosphorus and 53% of the total phosphorus, is largely concentrated in the testa-pericarp fraction (11). Despite the abundance of this material in the seed, very little is known of its physiological role. A suggestion by Atkinson and Morton (2) that phytic acid may be able to phosphorylate nucleotide diphosphates to triphosphates has some support from the work of Morton and Raison (13) and Biswas and Biswas (4), but this evidence is not unequivocable. Recently Sobolev and Rodionova (20) reported that phytic acid was synthesised by a mixture of aleurone grains and mitochondria isolated from ripening sunflower seeds when myoinositol and succinate were present. On addition of hexokinase and glucose to this mixture the amount of adenosine triphosphate in the medium fell, and no phytic acid was produced. Both sets of findings imply that the rate of synthesis of phytic acid is closely linked to the ATP level in the cell. For this reason the amounts of ATP and phytic acid in several morphologically distinct components of the wheat grain were determined from shortly after anthesis through to ripening. MATERIALS

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The catalytic effect of molybdate on the hydrolysis of organic phosphate bonds

Cited by 380 publications

References 10 publications

The presence of plasma membrane enzymes on the surface of bovine milk fat globules

The presence of plasma membrane enzymes on the surface of bovine milk fat globules

Synthesis of inorganic pyrophosphate by animal tissue mitochondria

The Role of Phytic Acid in the Wheat Grain

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