Abstract:Many Argonaute proteins can cleave RNA (″slicing″) as part of the microRNA-induced silencing complex (miRISC), even though miRNA-mediated target repression is generally independent of target cleavage. Here we useC. elegansto examine the role of miRNA-guided slicing in organismal development. We find that unwinding and decay of the miRNA star strand is weakly defective in the absence of slicing, with the largest effect observed in embryos. Argonaute-Like Gene 2 (ALG-2) is more dependent on slicing for unwinding… Show more
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