The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells

Moll, Roland; Franke, Werner W.; Schiller, Dorothea L.; Geiger, Benjamin; Krepler, R

doi:10.1016/0092-8674(82)90400-7

Cited by 4,944 publications

(3,595 citation statements)

References 59 publications

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“…Similarly, the E sheet positive control was thicker than the epithelial layers on the EC constructs, suggesting that nutrient availability, nutrient and oxygen diffusion or the presence of hMSCs altered epithelial layer development. Furthermore, expression of cytokeratins, an epithelial cell marker indicating structural cell integrity,23 was robust in the epithelial portion of the EC bilayer sheets and similar to that of the E control sheets cultured on standard cell culture inserts. The successful epithelial cell growth and differentiation at ALI and submerged in media achieved here is promising for engineering tubular tracheal replacements with a luminal epithelial coating.…”

Section: Discussionmentioning

confidence: 72%

A Modular Strategy to Engineer Complex Tissues and Organs

et al. 2018

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Currently, there are no synthetic or biologic materials suitable for long‐term treatment of large tracheal defects. A successful tracheal replacement must (1) have radial rigidity to prevent airway collapse during respiration, (2) contain an immunoprotective respiratory epithelium, and (3) integrate with the host vasculature to support epithelium viability. Herein, biopolymer microspheres are used to deliver chondrogenic growth factors to human mesenchymal stem cells (hMSCs) seeded in a custom mold that self‐assemble into cartilage rings, which can be fused into tubes. These rings and tubes can be fabricated with tunable wall thicknesses and lumen diameters with promising mechanical properties for airway collapse prevention. Epithelialized cartilage is developed by establishing a spatially defined composite tissue composed of human epithelial cells on the surface of an hMSC‐derived cartilage sheet. Prevascular rings comprised of human umbilical vein endothelial cells and hMSCs are fused with cartilage rings to form prevascular–cartilage composite tubes, which are then coated with human epithelial cells, forming a tri‐tissue construct. When prevascular– cartilage tubes are implanted subcutaneously in mice, the prevascular structures anastomose with host vasculature, demonstrated by their ability to be perfused. This microparticle–cell self‐assembly strategy is promising for engineering complex tissues such as a multi‐tissue composite trachea.

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Section: Discussionmentioning

confidence: 72%

A Modular Strategy to Engineer Complex Tissues and Organs

et al. 2018

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“…We suggest that apical cells of tadpoles are the counterpart of the periderm cells of mammalian embryos. One of the evidence supporting this suggestion is that the human periderm expresses keratin 8 (Moll et al, 1982), and apical cells express RK8, a Rana homologue of mammalian keratin 8 . We suggest that XAK-C is a Xenopus homologue of mammalian keratin 14, because both are expressed in epidermal basal cells.…”

Section: Discussionmentioning

confidence: 97%

“…Generally, keratins are useful markers to characterize and identify epithelial cells (Moll et al, 1982). We and other investigators used keratins as molecular probes to observe the metamorphic transformation of larval epidermal cells (Kinoshita and Sasaki, 1994a,b;Miller, 1996;Watanabe et al, 2001;Suzuki et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Metamorphosis‐dependent transcriptional regulation of xak‐c, a novel Xenopus type I keratin gene

Watanabe

Tanaka

Kobayashi

et al. 2002

Developmental Dynamics

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Anuran larvae transform their epidermis to the adult counterpart during metamorphosis. The major event of this process is the proliferation of larval epidermal basal cells and their differentiation into adult ones. The present study isolated novel type I keratin cDNA dubbed xak-c (Xenopus adult keratin-c) that was exclusively expressed in adult epidermal basal cells. The gene started its expression in the larval epidermis at the onset of metamorphosis. Thyroid hormone (TH) induced the precocious expression of the gene in the epidermis of premetamorphic tadpoles. To study the transcriptional regulation of this gene in relation to epidermal metamorphosis, a 2.8 kb 5 -flanking region of xak-c was cloned and its promoter activity was investigated. Gene constructs were made so as to contain the xak-c promoter region and gene of EGFP or luciferase as a reporter gene and were transfected into various types of cells, which revealed that the 5 -flanking region had an epidermal cellspecific transcriptional activity in both anurans and mammals. Larval skin tissues of Xenopus were transfected with the constructs and cultured in the presence and absence of TH, which showed that the promoter region is responsive to TH, although the region did not contain the consensus TH response element-like sequence. In sharp contrast, the promoter region did not respond to TH in the adult skin, clearly indicating that the cloned region contains specific sequences that respond to metamorphosis-dependent transcription factor(s).

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“…Keratin, a naturally occurring polymer, is biorenewable, biodegradable, biocompatible, and biofunctional. 12 14 The use of keratin in tissue engineering scaffolds (e.g., hydrated gel or sponges) has been shown to enhance cell attachment and proliferation, and to improve the biomaterial's cellular and tissue biocompatibility, both in vitro 15 and in vivo. 12,16 Although these recent efforts in keratinbased biomaterials are encouraging, much remains to be explored and improved, particularly in terms of the wide range of biomedical applications where mechanical strength and integrity of nanofibers in aqueous medium are important.…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)/keratin-based composite nanofibers for biomedical applications

Edwards

Jarvis

Hopkins

et al. 2014

J. Biomed. Mater. Res.

111

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Keratin-based composite nanofibers have been fabricated by an electrospinning technique. Aqueous soluble keratin extracted from human hair was successfully blended with poly(e-caprolactone) (PCL) in different ratios and transformed into nanofibrous membranes. Toward the potential use of this nanofibrous membrane in tissue engineering, its physicochemical properties, such as morphology, mechanical strength, crystallinity, chemical structure, and integrity in aqueous medium were studied and its cellular compatibility was determined. Nanofibrous membranes with PCL/keratin ratios from 100/00 to 70/30 showed good uniformity in fiber morphology and suitable mechanical properties, and retained the integrity of their fibrous structure in buffered solutions.Experimental results, using cell viability assays and scanning electron microscopy imaging, showed that the nanofibrous membranes supported 3T3 cell viability. The ability to produce blended nanofibers from protein and synthetic polymers represents a significant advancement in development of composite materials with structural and material properties that will support biomedical applications. This provides new nanofibrous materials for applications in tissue engineering and regenerative medicine.

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The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells

Cited by 4,944 publications

References 59 publications

A Modular Strategy to Engineer Complex Tissues and Organs

A Modular Strategy to Engineer Complex Tissues and Organs

Metamorphosis‐dependent transcriptional regulation of xak‐c, a novel Xenopus type I keratin gene

Poly(ε-caprolactone)/keratin-based composite nanofibers for biomedical applications

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