The Catalase-Peroxidase KatG Is Required for Virulence of
 Xanthomonas campestris
 pv. campestris in a Host Plant by Providing Protection against Low Levels of H
 2
 O
 2

Jittawuttipoka, Thichakorn; Buranajitpakorn, Sarinya; Vattanaviboon, Paiboon; Mongkolsuk, Skorn

doi:10.1128/jb.00788-09

Cited by 47 publications

(57 citation statements)

References 32 publications

(37 reference statements)

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“…A similar pattern of catalase function is seen in X. campestris pv. campestris, a pathogen that has two monofunctional catalases, KatA and KatE, and a bifunctional catalase, KatG (21). In our studies, DC3000 KatE does provide a small amount of catalase activity, as the katB katG double mutant has higher catalase activity than the triple mutant (Fig.…”

Section: Discussionmentioning

confidence: 76%

“…This nonredundant role of catalases in virulence is also seen in X. campestris pv. campestris, in which both katA and katG single mutants are avirulent in its host Chinese radish (Raphanus sativus) (21). Catalases have also been shown to be important in the virulence of other plant-pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Other bifunctional catalases have been shown to play a major role in detoxifying menadione, including KatG from X. campestris pv. campestris strain ATCC 33913 (21) and KatA (a homolog of KatG) from A. tumefaciens NTL4 (35). It is possible that the enhanced ability of bifunctional catalases to detoxify menadione arises from an ability to remove organic hydroperoxides generated by menadione.…”

Section: Discussionmentioning

confidence: 99%

“…campestris uses KatA and KatG, and Xanthomonas axonopodis pv. citri uses KatE (21,45,49). Interestingly, the symbiotic bacterium Sinorhizobium meliloti uses both mono-and bifunctional catalases to aid its symbiosis (20,41).…”

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confidence: 99%

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Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis

et al. 2012

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ABSTRACTThe bacterial pathogenPseudomonas syringaepv. tomato DC3000 must detoxify plant-produced hydrogen peroxide (H2O2) in order to survive in its host plant. Candidate enzymes for this detoxification include the monofunctional catalases KatB and KatE and the bifunctional catalase-peroxidase KatG of DC3000. This study shows that KatG is the major housekeeping catalase of DC3000 and provides protection against menadione-generated endogenous H2O2. In contrast, KatB rapidly and substantially accumulates in response to exogenous H2O2. Furthermore, KatB and KatG have nonredundant roles in detoxifying exogenous H2O2and are required for full virulence of DC3000 inArabidopsis thaliana. Therefore, the nonredundant ability of KatB and KatG to detoxify plant-produced H2O2is essential for the bacteria to survive in plants. Indeed, a DC3000 catalase triple mutant is severely compromised in its ability to growin planta, and its growth can be partially rescued by the expression ofkatB,katE, orkatG. Interestingly, our data demonstrate that although KatB and KatG are the major catalases involved in the virulence of DC3000, KatE can also provide some protectionin planta. Thus, our results indicate that these catalases are virulence factors for DC3000 and are collectively required for pathogenesis.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis

et al. 2012

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“…The catalase-peroxidase enzyme, KatG, 3 is found in many microorganisms but in the human pathogen Mycobacterium tuberculosis (M. tb) it is the only "catalase" (2 H 2 O 2 3 O 2 ϩ 2 H 2 O) and plays an important role in protecting this organism against oxidative stress (1)(2). The structural origins of its high catalase activity are therefore of special interest since KatG may fulfill a role different from the ubiquitous monofunctional catalases, especially in organisms that contain genes for both the monofunctional and dual function enzymes (3).…”

mentioning

confidence: 99%

Specific Function of the Met-Tyr-Trp Adduct Radical and Residues Arg-418 and Asp-137 in the Atypical Catalase Reaction of Catalase-Peroxidase KatG

Zhao

Khajo

Jarrett

et al. 2012

Journal of Biological Chemistry

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Background: Catalase activity in catalase-peroxidases occurs through a poorly defined mechanism requiring residues Arg-418, Asp-137, and the amino acid adduct (M 255 Y 229 W 107 ). Results: Along with an adduct radical, Arg-418 stimulates O 2 release from dioxyheme, the rapid formation of which requires Asp-137. Conclusion:The electronics of the Arg-418:MYW-radical interaction enables discharge of O 2 during H 2 O 2 turnover. Significance: An atypical catalase mechanism operates in KatG.

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Mutations of ferric uptake regulator (fur) impair iron homeostasis, growth, oxidative stress survival, and virulence of Xanthomonas campestris pv. campestris

et al. 2010

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Iron is essential in numerous cellular functions. Intracellular iron homeostasis must be maintained for cell survival and protection against iron's toxic effects. Here, we characterize the roles of Xanthomonas campestris pv. campestris (Xcc) fur, which encodes an iron sensor and a transcriptional regulator that acts in iron homeostasis, oxidative stress, and virulence. Herein, we isolated spontaneous Xcc fur mutants that had high intracellular iron concentrations due to constitutively high siderophore levels and increased expression of iron transport genes. These mutants also had reduced aerobic plating efficiency and resistance to peroxide killing. Moreover, one fur mutant was attenuated on a host plant, thus indicating that fur has important roles in the virulence of X. campestris pv. campestris.

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The Catalase-Peroxidase KatG Is Required for Virulence of Xanthomonas campestris pv. campestris in a Host Plant by Providing Protection against Low Levels of H ₂ O ₂

Cited by 47 publications

References 32 publications

Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis

Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis

Specific Function of the Met-Tyr-Trp Adduct Radical and Residues Arg-418 and Asp-137 in the Atypical Catalase Reaction of Catalase-Peroxidase KatG

Mutations of ferric uptake regulator (fur) impair iron homeostasis, growth, oxidative stress survival, and virulence of Xanthomonas campestris pv. campestris

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The Catalase-Peroxidase KatG Is Required for Virulence of Xanthomonas campestris pv. campestris in a Host Plant by Providing Protection against Low Levels of H 2 O 2

Cited by 47 publications

References 32 publications

Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis

Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis

Specific Function of the Met-Tyr-Trp Adduct Radical and Residues Arg-418 and Asp-137 in the Atypical Catalase Reaction of Catalase-Peroxidase KatG

Mutations of ferric uptake regulator (fur) impair iron homeostasis, growth, oxidative stress survival, and virulence of Xanthomonas campestris pv. campestris

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The Catalase-Peroxidase KatG Is Required for Virulence of Xanthomonas campestris pv. campestris in a Host Plant by Providing Protection against Low Levels of H ₂ O ₂