The Catabolism of Phenylacetic Acid and Other Related Molecules in Pseudomonas putida U

Luengo, José M.; Arias, Sagrario; Arcos, Mario; Olivera, Elı́as R.

doi:10.1007/978-1-4020-6097-7_6

Cited by 5 publications

(5 citation statements)

References 110 publications

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“…The transcription of two genes (Sso2067 and Sso2069) encoding for both subunits of this protein was found to be significantly increased in medium containing caseinhydrolysate. Presumably, the acetate-CoA ligase homologue (Sso1806) is again responsible for the formation of phenylacetate (as was shown for P. aerophilum [41]) and 4-hydroxyphenylacetate from their respective CoA [44]. All in all, the Stickland-like incomplete oxidation of aromatic amino acids observed in this work has not yet been reported for obligate aerobic organisms.…”

Section: Aromatic Amino Acids Are Catabolised To Organic Acids Via Oxsupporting

confidence: 52%

“…Phenylacetate, which could only be detected in cells grown on caseinhydrolysate (Table ), can be further metabolised to 2‐hydroxyphenylacetate (and also to 4‐hydroxyphenylacetate) via action of monooxygenases. The best relevant candidate for a 4‐hydroxyphenylacetate‐3‐hydroxylase (Sso2053) shares high sequence similarity to the enzyme from Pseudomonas putida (identity: 37%, e ‐value: 4·10 −90 ) which was shown to exhibit a broad substrate specificity including phenylacetate . All in all, the Stickland‐like incomplete oxidation of aromatic amino acids observed in this work has not yet been reported for obligate aerobic organisms.…”

Section: Resultsmentioning

confidence: 75%

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Oxidative Stickland reactions in an obligate aerobic organism – amino acid catabolism in the Crenarchaeon Sulfolobus solfataricus

et al. 2017

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The thermoacidophilic Crenarchaeon Sulfolobus solfataricus is a model organism for archaeal adaptation to extreme environments and renowned for its ability to degrade a broad variety of substrates. It has been well characterised concerning the utilisation of numerous carbohydrates as carbon source. However, its amino acid metabolism, especially the degradation of single amino acids, is not as well understood. In this work, we performed metabolic modelling as well as metabolome, transcriptome and proteome analysis on cells grown on caseinhydrolysate as carbon source in order to draw a comprehensive picture of amino acid metabolism in S. solfataricus P2. We found that 10 out of 16 detectable amino acids are imported from the growth medium. Overall, uptake of glutamate, methionine, leucine, phenylalanine and isoleucine was the highest of all observed amino acids. Our simulations predict an incomplete degradation of leucine and tyrosine to organic acids, and in accordance with this, we detected the export of branched‐chain and aromatic organic acids as well as amino acids, ammonium and trehalose into the culture supernatants. The branched‐chain amino acids as well as phenylalanine and tyrosine are degraded to organic acids via oxidative Stickland reactions. Such reactions are known for prokaryotes capable of anaerobic growth, but so far have never been observed in an obligate aerobe. Also, 3‐methyl‐2‐butenoate and 2‐methyl‐2‐butenoate are for the first time found as products of modified Stickland reactions for the degradation of branched‐chain amino acids. This work presents the first detailed description of branched‐chain and aromatic amino acid catabolism in S. solfataricus.

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Section: Aromatic Amino Acids Are Catabolised To Organic Acids Via Oxsupporting

confidence: 52%

Section: Resultsmentioning

confidence: 75%

Oxidative Stickland reactions in an obligate aerobic organism – amino acid catabolism in the Crenarchaeon Sulfolobus solfataricus

et al. 2017

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“…putida U [ 142 ]. The degradation of those polymers, which have plastic properties, is carried out when the carbon source has been exhausted from the media, thus providing this bacterium with advantages for its survival [ 143 ]. Also, some putative substrates of this pathway, like styrene, are toxic compounds [ 142 ].…”

Section: Resultsmentioning

confidence: 99%

Genomic insights into the broad antifungal activity, plant-probiotic properties, and their regulation, in Pseudomonas donghuensis strain SVBP6

2018

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Plant-growth promotion has been linked to the Pseudomonas genus since the beginning of this research field. In this work, we mined the genome of an Argentinean isolate of the recently described species P. donghuensis. Strain SVBP6, isolated from bulk soil of an agricultural plot, showed a broad antifungal activity and several other plant-probiotic activities. As this species has been recently described, and it seems like some plant-growth promoting (PGP) traits do not belong to the classical pseudomonads toolbox, we decide to explore the SVBP6 genome via an bioinformatic approach. Genome inspection confirmed our previous in vitro results about genes involved in several probiotic activities. Other genetic traits possibly involved in survival of SVBP6 in highly competitive environments, such as rhizospheres, were found. Tn5 mutagenesis revealed that the antifungal activity against the soil pathogen Macrophomina phaseolina was dependent on a functional gacS gene, from the regulatory cascade Gac-Rsm, but it was not due to volatile compounds. Altogether, our genomic analyses and in vitro tests allowed the phylogenetic assignment and provided the first insights into probiotic properties of the first P. donghuensis isolate from the Americas.

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“…In bacteria, metabolic pathways of PA assimilation have been widely studied and considered to follow either of four possible routes ( Figure 1). Utilization of PA via hydroxy intermediates (2-, 3-, or 4-hydroxyphenylacetic acid, Figure 1, route a, b or c, respectively) has been proposed in several microorganisms like Aspergillus, Nocardia, Flavobacterium, Trichosporon, Streptomyces, a chloridazon-degrading bacterium, P. putida, E. coli, Klebsiella, Acinetobacter and other unrelated microbes [6][7][8][9][10][11][12]. The metabolism of PA via hydroxylation of the aromatic ring was proposed in these strains.…”

Section: Introductionmentioning

confidence: 99%

“…An unconventional 'aerobic hybrid pathway' of PA catabolism encoded by paa gene cluster by phenylacetyl-CoA ligase ( Figure 1, route d). This key enzyme has unequivocally been shown to be directly involved in the degradation of PA and its presence has been observed across the bacterial phylum [5,7,[18][19][20][21]. A series of CoA-thioester intermediates have been involved in this pathway which ultimately leads to the formation of succinyl-CoA and acetyl-CoA that enters the central metabolic pathway [1] ( Figure 1, route d).…”

Section: Introductionmentioning

confidence: 99%

Metabolism and Preferential Utilization of Phenylacetic acid and 4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86

Shrivastava¹,

Purohit²,

Phale³

2011

J Bioremed Biodegrad

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Metabolic pathways for phenylaceticacid (PA) and 4-hydroxyphenylacetic acid (4-HPA) from Pseudomonas putida CSV86 were elucidated. PA grown strain CSV86 cells showed higher oxygen-uptake on PA as compared to hydroxyphenylacetic acids. Detection of phenylacetyl-CoA ligase and absence of 4-hydroxyphenylacetic acid hydroxylase (4HPAH) and 3,4-dihydroxyphenylacetic acid-dioxygenase (3,4-DHPADO) activity supports this observation. 4-HPA grown cells showed oxygen-uptake on 4-HPA and 3,4-dihydroxyphenylacetic acid (3,4-DHPA) but showed significantly lower respiration rates on 2,5-dihydroxyphenylacetic acid and PA. Detection of 4HPAH and 3,4-DHPADO activities support the respiration data. Metabolic studies indicate that strain CSV86 metabolizes PA via phenylacetyl-CoA while 4-HPA via 3,4-DHPA pathway. Glucose grown cells showed lower activity of enzymes and respiration on PA, 4-HPA and 3,4-DHPA, suggesting that pathways are inducible. When grown on double carbon sources such as PA or 4-HPA plus glucose, CSV86 cells showed diauxic growth pattern with oxygen uptake on PA, 4-HPA and 3,4-DHPA in the first log-phase which was reduced during the second log-phase with increase in the respiration rate on glucose. This observation was supported by detection of high 4HPAH and 3,4-DHPADO activity in the first log-phase and glucose-6-phosphate dehydrogenase activity in the second log-phase. These results suggest that strain CSV86 utilizes PA and 4-HPA preferentially over glucose. Metabolism and Preferential Utilization of

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The Catabolism of Phenylacetic Acid and Other Related Molecules in Pseudomonas putida U

Cited by 5 publications

References 110 publications

Oxidative Stickland reactions in an obligate aerobic organism – amino acid catabolism in the Crenarchaeon Sulfolobus solfataricus

Oxidative Stickland reactions in an obligate aerobic organism – amino acid catabolism in the Crenarchaeon Sulfolobus solfataricus

Genomic insights into the broad antifungal activity, plant-probiotic properties, and their regulation, in Pseudomonas donghuensis strain SVBP6

Metabolism and Preferential Utilization of Phenylacetic acid and 4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86

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