The capillary dilator substances in dry powders of spinal roots; a possible role of adenosine triphosphate in chemical transmission from nerve endings

Holton, F. A.; Holton, Pamela

doi:10.1113/jphysiol.1954.sp005198

Cited by 209 publications

(85 citation statements)

References 24 publications

(41 reference statements)

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“…This substance has already been implicated in the transmission of dorsal root antidromic vasodilatation (Holton and Holton, 1954). However, as reported by Bueding et al (1967).…”

mentioning

confidence: 40%

Effects of Imidazole and Phentolamine on the Relaxant Responses of Guinea-pig Taenia Coli to Transmural Stimulation and to Adenosine Triphosphate

Rikimaru

Fukushi

Suzuki

1971

Tohoku J. Exp. Med.

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“…This substance has already been implicated in the transmission of dorsal root antidromic vasodilatation (Holton and Holton, 1954). However, as reported by Bueding et al (1967).…”

mentioning

confidence: 40%

Effects of Imidazole and Phentolamine on the Relaxant Responses of Guinea-pig Taenia Coli to Transmural Stimulation and to Adenosine Triphosphate

Rikimaru

Fukushi

Suzuki

1971

Tohoku J. Exp. Med.

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“…The P21 receptor is the dominant subtype of purinoceptor in rat urinary bladder (Bo & Burnstock, 1990). These findings do not exclude the possibility that in other species or in other tissues ATP may contribute to the efferent function of sensory nerves (Holton & Holton, 1954;Sweeney et al, 1989) and further studies on this topic are warranted.…”

Section: Discussionmentioning

confidence: 97%

“…ATP produces a rapidly developing phasic bladder contraction and is considered a likely noncholinergic excitatory transmitter at this level (Brown et al, 1979;Bo & Burnstock, 1990;Brading & Williams, 1990;Luheshi & Zar, 1990). After the early suggestion of Holton & Holton (1954) and Holton (1959), a role for ATP as a sensory transmitter has been recently reconsidered (Jahr & Jessell, 1983;Fyffe & Perl, 1984). In particular, it appears that certain primary sensory neurones which are capsaicin-sensitive may utilize ATP as a transmitter (Nagy & Daddona, 1985) and ATP release by capsaicin in the dorsal half of spinal cord has been reported upon application of capsaicin (Sweeney et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Tachykinin antagonists and capsaicin‐induced contraction of the rat isolated urinary bladder: evidence for tachykinin‐mediated cotransmission

Maggi

Patacchini

Santicioli

et al. 1991

British J Pharmacology

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1 The possible involvement of tachykinins (TKs) in the contraction produced by capsaicin in the rat isolated urinary bladder was addressed on the hypothesis that co-release of substance P (SP) and neurokinin A (NKA) occurs from sensory nerve terminals. 2 A low concentration of SP (30 nM) produced a rapid contraction which faded to baseline within 10min. A low concentration of NKA (10nM) produced a slowly developing contraction which was still evident at 10min. Capsaicin (1 pM) produced a rapid phasic response and a tonic response (late response to capsaicin). Co-administration of SP and NKA mimicked the response to capsaicin more than each TK alone. 3 Fading of the response to SP was not caused by receptor desensitization and was partially prevented by peptidase inhibitors.4 Spantide (3pM) selectively antagonized the SP-induced contraction while L-659,877 (3-lOpM) or MEN 10,376 (10-30pM) which are NK2 receptor selective antagonists selectively blocked the response to NKA.Co-administration of spantide and L-659,877 inhibited the response to both SP and NKA by an amount not greater than that produced by each antagonist alone. 5 Spantide selectively reduced the peak response to capsaicin, while leaving the late response unaffected.L-659,877 (3 pM) and MEN 10,376 (10pM) selectively inhibited the late response to capsaicin while, at higher concentrations, also reduced the peak response to capsaicin. Co-administration of spantide and L-659,877 reduced the peak response to capsaicin more than that produced by each antagonist alone. 6 Bombesin (10nM) produced a tonic contraction similar to that induced by NKA. The response to bombesin was not affected by spantide, L-659,877 or MEN 10,376. 7 P2. purinoceptor desensitization by repeated administration of a,fl-methylene ATP depressed the twitch response to electrical stimulation of postganglionic nerves but did not affect the peak or the late response to capsaicin. 8 We conclude that multiple TKs are coreleased by capsaicin in the rat bladder and mediate the capsaicin-induced contraction by activating both NKI and NK2 receptors. Endogenous TK with preferential affinity for the NK, receptor (putatively SP) are selectively involved in the peak response to capsaicin while endogenous TK with preferential affinity for the NK2 receptor (putatively NKA) are selectively involved in the late response to capsaicin and partly contribute to the peak response. These findings provide pharmacological evidence for tachykinin-mediated cotransmission in the rat urinary bladder. ATP is unlikely to be involved in the efferent function of capsaicin-sensitive sensory nerves in the rat bladder.

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“…To release Ca2+ , Ins(l,4,5)P3 must bind to receptors that are somehow linked to Ca2 + channels connected with Ins( 1,4,5)P3-sensitive pools. Heparin inhibits both the binding of Ins (1,4,5)P3 to its purified receptor [ 151 and the mobilization of Ca2 + in numerous cell systems [16]. Fig 3B shows that intra-oocyte injection of heparin (final concentration = 80 PM) blocked the ATP responsiveness (n = 4).…”

Section: Resultsmentioning

confidence: 99%

“…The Ins(l,4,5)P3 response of this Ca2' mobilizing neurotransmitter may be considered as a reference positive control since the muscarinic receptor transduction pathway has been established in previous studies as a Ca2 + release from Ins(l,4,5)P3-sensitive Ca2 + stores in defolliculated oocytes injected with mamm~ian brain mRNA [14]. Moreover, Ins ( 1,4,5)P3 level remained unchanged in native oocytes in the presence of ATP (300 PM; data not shown; 3 experiments). To release Ca2+ , Ins(l,4,5)P3 must bind to receptors that are somehow linked to Ca2 + channels connected with Ins( 1,4,5)P3-sensitive pools.…”

Section: Resultsmentioning

confidence: 99%

Ins(1,4,5)P₃ formation and fluctuating chloride current response induced by external ATP in Xenopus oocytes injected with embryonic guinea pig brain mRNA

Fournier¹,

Honoré²,

Collin³

et al. 1990

FEBS Letters

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In voltage-clamped Xenopus oocytes injected with embryonic guinea pig mRNA, effective concentrations of extracellular ATP elicited an inward fluctuating current. This current, carried by Cl-ions, was mainly dependent upon liberation of Caz+ ions from stores as demonstrated by experiments using intracellular EGTA loading and TMB-8 superfusion. Neomycin inhibited these fluctuating currents indicating that the transplanted purinoceptor is linked to phospholipase C activity and triggers Ins(l,4,5)P, formation. Ins(l,4,5)P, production evoked by external ATP was clearly demonstrated by directly measuring the water-soluble Ins(l,4,5)P, level in injected oocytes. Finally, it is suggested that the ATP effect was mediated by a Ca*+ release from Ins(l,4,5)P, sensitive pools since heparin blocked the ATP responsiveness. The acquired purinoceptor may be made apparent to a P2 subtype since ATP and ADP were equipotent in eliciting Cl-current while AMP and Adenosine were ineffective in injected oocytes.

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The capillary dilator substances in dry powders of spinal roots; a possible role of adenosine triphosphate in chemical transmission from nerve endings

Cited by 209 publications

References 24 publications

Effects of Imidazole and Phentolamine on the Relaxant Responses of Guinea-pig Taenia Coli to Transmural Stimulation and to Adenosine Triphosphate

Effects of Imidazole and Phentolamine on the Relaxant Responses of Guinea-pig Taenia Coli to Transmural Stimulation and to Adenosine Triphosphate

Tachykinin antagonists and capsaicin‐induced contraction of the rat isolated urinary bladder: evidence for tachykinin‐mediated cotransmission

Ins(1,4,5)P₃ formation and fluctuating chloride current response induced by external ATP in Xenopus oocytes injected with embryonic guinea pig brain mRNA

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The capillary dilator substances in dry powders of spinal roots; a possible role of adenosine triphosphate in chemical transmission from nerve endings

Cited by 209 publications

References 24 publications

Effects of Imidazole and Phentolamine on the Relaxant Responses of Guinea-pig Taenia Coli to Transmural Stimulation and to Adenosine Triphosphate

Effects of Imidazole and Phentolamine on the Relaxant Responses of Guinea-pig Taenia Coli to Transmural Stimulation and to Adenosine Triphosphate

Tachykinin antagonists and capsaicin‐induced contraction of the rat isolated urinary bladder: evidence for tachykinin‐mediated cotransmission

Ins(1,4,5)P3 formation and fluctuating chloride current response induced by external ATP in Xenopus oocytes injected with embryonic guinea pig brain mRNA

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Product

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Ins(1,4,5)P₃ formation and fluctuating chloride current response induced by external ATP in Xenopus oocytes injected with embryonic guinea pig brain mRNA