The capability of the fungus Mucor circinelloides to maintain parasiticidal activity after the industrial feed pelleting enhances the possibilities of biological control of livestock parasites

Arroyo, F.L.; Arias, M.; Cazapal-Monteiro, C.; Hernández, José Ángel; Suárez, J. L.; Miguélez, Silvia; Romasanta, A.; Sánchez-Andrade, R.; Silva, Adolfo Paz

doi:10.1016/j.biocontrol.2015.09.007

Cited by 17 publications

(9 citation statements)

References 17 publications

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“…The demonstration of spores of M. circinelloides and D. flagrans which survived the industrial manufacturing of pelleted feed offers a very useful solution to their distribution and provides also a practical approach to implement strategies of integrated control [ 35 , 36 ]. To our knowledge, this is the first investigation concerning the feeding of captive animals with pellets containing spores of parasiticide fungi.…”

Section: Discussionmentioning

confidence: 99%

Implementation of Biological Control to the Integrated Control of Strongyle Infection among Wild Captive Equids in a Zoological Park

Palomero

Hernández

Cazapal-Monteiro

et al. 2018

BioMed Research International

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The integrated control of strongyles was assayed for a period of three years in wild equids (zebras, European donkeys, and African wild asses) captive in a zoo and infected by strongyles. During three years control of parasites consisted of deworming with ivermectin + praziquantel; equids also received every two days commercial nutritional pellets containing a blend of 104 - 105 spores of the fungi Mucor circinelloides + Duddingtonia flagrans per kg meal. Coprological analyses were done monthly to establish the counts of eggs of strongyles per gram of feces (EPG). The reductions in the fecal egg counts (FECR) and in the positive horses (PHR) were calculated fifteen days after deworming; the egg reappearance period (ERP) and the time elapsed from the previous deworming (TPD) were also recorded. Four anthelmintic treatments were administered during the assay, three times throughout the first 2 yrs, and another treatment during the last one. FECR values of 96-100% and 75-100% for the PHR were recorded. The ERP oscillated between eight and twenty-eight weeks, and the TPD ranged from four to eighteen months, increasing to the end of the trial. No side effects were observed in any of the equids. It is concluded that integrated control of strongyles among equids captive in a zoo can be developed by anthelmintic deworming together with the administration of pellets manufactured with spores of parasiticide fungi every two days.

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Section: Discussionmentioning

confidence: 99%

Implementation of Biological Control to the Integrated Control of Strongyle Infection among Wild Captive Equids in a Zoological Park

Palomero

Hernández

Cazapal-Monteiro

et al. 2018

BioMed Research International

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“…Therefore, administration of those spores has been advised as an appropriate procedure to prevent horse infection by strongyles [ 11 , 12 ]. As occurs with other ovicidal fungi, Mucor circinelloides can attach to the egg-shell of the parasite, penetrate inside and destroy the inner content [ 13 , 14 ]. An antagonistic effect of M. circinelloides has been shown on the eggs of helminths such as Fasciola hepatica and Ascaris suum that are shed in feces of cattle and pigs, respectively [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…An antagonistic effect of M. circinelloides has been shown on the eggs of helminths such as Fasciola hepatica and Ascaris suum that are shed in feces of cattle and pigs, respectively [ 15 ]. Recently, the manufacturing of pelleted feed with spores of M. circinelloides and D. flagrans has been suggested as an easy way to ensure the presence of fungal stages in the feces, where strongyle eggs hatch and develop to larvae [ 14 , 16 ]. Herein, the possibility of enhancing the beneficial effect of rotational grazing by providing pelleted feed manufactured with the spores of M. circinelloides and D. flagrans has been evaluated.…”

Section: Introductionmentioning

confidence: 99%

A combined effort to avoid strongyle infection in horses in an oceanic climate region: rotational grazing and parasiticidal fungi

et al. 2018

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BackgroundAn approach to preventing strongyle infection in horses was tested, comprising rotational pasturing and the administration of spores of two parasiticidal fungi, Mucor circinelloides and Duddingtonia flagrans.MethodsTwenty-two adult Spanish Sport Horses were dewormed with ivermectin (1 mg pour-on/kg body weight) and then randomly divided into three groups. G-1 was maintained with continuous grazing, and G-2 and G-3 were kept on a four-paddock rotation system. Commercial pelleted feed (2.5 kg/horse) was supplied to G-1 and G-2 twice a week; horses in G-3 received pellets containing 2 × 106 spores/kg of each fungus. Fecal samples were analyzed by the flotation method to estimate the reduction in the fecal egg counts (FECR), the percentage of horses shedding eggs (PHR), and the egg reappearance period (ERP).ResultsThird-stage larvae were identified in fecal pats as Cyathostomum (sensu lato) types A, C and D, Gyalocephalus capitatus, Triodontophorus serratus, Poteriosthomum spp., Strongylus vulgaris and S. edentatus. Two weeks after treatment, the FECR values were 100% in G-1, 96% in G-2 and 99% in G-3; the PHR values were 100% in G-1, 75% in G-2 and 88% in G-3. A strongyle ERP of 6 weeks was observed in G-1, ERP of 10 weeks was observed in G-2, and ERP of 16 weeks was observed in G-3. The counts of eggs per gram of feces (EPG) were > 300 EPG in G-1 and G-2 but remained below 250 EPG in G-3 throughout the observation period of 12 months.ConclusionsThese results suggest that horse strongyle infection could be decreased by combining rotational pasturing with feeding pellets containing the spores of parasiticidal fungi.

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“…Most known species with ovicidal activity are Verticillium spp., Pochonia chlamydosporia , Paecilomyces lilacinus , Trichoderma spp., or Mucor circinelloides [ 18 , 19 , 20 ]. By developing the phases of adhesion, colonization, penetration, and deliberation, these fungi develop an ovicidal activity [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Ovicidal Fungi from Fecal Samples of Captive Animals Maintained in a Zoological Park

Hernández

Vázquez‐Ruiz

Cazapal-Monteiro

et al. 2017

JoF

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There are certain saprophytic fungi in the soil able to develop an antagonistic effect against eggs of parasites. Some of these fungal species are ingested by animals during grazing, and survive in their feces after passing through the digestive tract. To identify and isolate ovicidal fungi in the feces of wild captive animals, a total of 60 fecal samples were taken from different wild animals kept captive in the Marcelle Natureza Zoological Park (Lugo, Spain). After the serial culture of the feces onto Petri dishes with different media, their parasicitide activity was assayed against eggs of trematodes (Calicophoron daubneyi) and ascarids (Parascaris equorum). Seven fungal genera were identified in the feces. Isolates from Fusarium, Lecanicillium, Mucor, Trichoderma, and Verticillium showed an ovicidal effect classified as type 3, because of their ability to adhere to the eggshell, penetrate, and damage permanently the inner embryo. Penicillium and Gliocladium developed a type 1 effect (hyphae attach to the eggshell but morphological damage was not provoked). These results provide very interesting and useful information about fungi susceptible for being used in biological control procedures against parasites.

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The capability of the fungus Mucor circinelloides to maintain parasiticidal activity after the industrial feed pelleting enhances the possibilities of biological control of livestock parasites

Cited by 17 publications

References 17 publications

Implementation of Biological Control to the Integrated Control of Strongyle Infection among Wild Captive Equids in a Zoological Park

Implementation of Biological Control to the Integrated Control of Strongyle Infection among Wild Captive Equids in a Zoological Park

A combined effort to avoid strongyle infection in horses in an oceanic climate region: rotational grazing and parasiticidal fungi

Isolation of Ovicidal Fungi from Fecal Samples of Captive Animals Maintained in a Zoological Park

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