The canonical Wnt pathway in early mammalian embryogenesis and stem cell maintenance/differentiation

Wang, Jianbo; Wynshaw‐Boris, Anthony

doi:10.1016/j.gde.2004.07.013

Cited by 111 publications

(75 citation statements)

References 51 publications

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“…FGF and Wnt family members are important mammalian developmental and stem cell signals (43,44). FGF signaling is crucial for hESC maintenance (20).…”

Section: Discussionmentioning

confidence: 99%

Defined culture conditions of human embryonic stem cells

Booth³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

188

118

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Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by the difficulties of eliminating animal products in the culture medium and͞or the complexity of conditions required to support hESC growth. We have developed a simple medium [termed hESC Cocktail (HESCO)] containing basic fibroblast growth factor, Wnt3a, April (a proliferation-inducing ligand)͞BAFF (B cell-activating factor belonging to TNF), albumin, cholesterol, insulin, and transferrin, which is sufficient for hESC self-renewal and proliferation. Cells grown in HESCO were maintained in an undifferentiated state as determined by using six different stem cell markers, and their genomic integrity was confirmed by karyotyping. Cells cultured in HESCO readily form embryoid bodies in tissue culture and teratomas in mice. In both cases, the cells differentiated into each of the three cell lineages, ectoderm, endoderm, and mesoderm, indicating that they maintained their pluripotency. The use of a minimal medium sufficient for hESC growth is expected to greatly facilitate clinical application and developmental studies of hESCs.April͞BAFF ͉ fibroblast growth factor ͉ serum free culture ͉ wnt

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“…FGF and Wnt family members are important mammalian developmental and stem cell signals (43,44). FGF signaling is crucial for hESC maintenance (20).…”

Section: Discussionmentioning

confidence: 99%

Defined culture conditions of human embryonic stem cells

Booth³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

188

118

View full text Add to dashboard Cite

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“…The BAT nlacZ model used in this study visualizes only the canonical Wnt response (Maretto et al, 2003), which involves stabilization and nuclear translocation of ␤catenin (Wang and Wynshaw-Boris, 2004;Widelitz, 2005). In general, members of the Wnt1 subclass (including Wnt1, Wnt2, Wnt3, Wnt7, and Wnt8) are potent activators of this pathway, whereas members of the Wnt5a subclass (including Wnt4, Wnt5, Wnt8, and Wnt11) are poor activators or, in some cases, inhibitors (Torres et al, 1996;Yamaguchi, 2001;Church et al, 2002;Oishi et al, 2003;Topol et al, 2003;Maye et al, 2004;Tian et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Activation of theWnt–βCatenin Pathway in a Cell Population on the Surface of the Forebrain Is Essential for the Establishment of Olfactory Axon Connections

Zaghetto¹,

Paina²,

Mantero³

et al. 2007

J. Neurosci.

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A variety of signals governing early extension, guidance, and connectivity of olfactory receptor neuron (ORN) axons has been identified; however, little is known about axon-mesoderm and forebrain (FB)-mesoderm signals. Using Wnt-␤catenin reporter mice, we identify a novel Wnt-responsive resident cell population, located in a Frizzled7 expression domain at the surface of the embryonic FB, along the trajectory of incoming ORN axons. Organotypic slice cultures that recapitulate olfactory-associated Wnt-␤catenin activation show that the ␤catenin response depends on a placode-derived signal(s). Likewise, in Dlx5Ϫ/Ϫ embryos, in which the primary connections fail to form, Wnt-␤catenin response on the surface of the FB is strongly reduced. The olfactory placode expresses a number of ␤catenin-activating Wnt genes, and the Frizzled7 receptor transduces the "canonical" Wnt signal; using Wnt expression plasmids we show that Wnt5a and Wnt7b are sufficient to rescue ␤catenin activation in the absence of incoming axons. Finally, blocking the canonical Wnt pathway with the exogenous application of the antagonists Dikkopf-1 or secreted-Frizzled-receptor protein-2 prevents ORN axon contact to the FB. These data reveal a novel function for Wnt signaling in the establishment of periphery-CNS olfactory connections and highlight a complex interplay between cells of different embryonic origin for ORN axon connectivity.

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“…The canonical wnt signaling pathway involves the intracellular activity of B-catenin and a cyclin D1 target (Wang and Wynshaw-Boris, 2004). Suppression of wnt signaling in the zebrafish results in failure of retinal progenitors to incorporate BrdU, suggesting a requirement for this signaling pathway in promoting passage through the cell cycle .…”

Section: Extrinsic Factorsmentioning

confidence: 99%

Neurogenesis in the Fish Retina

Stenkamp

2007

International Review of Cytology

122

136

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The retinas of teleost fish have long been of interest to developmental neurobiologists for their persistent plasticity during growth, life history changes, and response to injury. Because the vertebrate retina is a highly conserved tissue, the study of persistent plasticity in teleosts has provided insights into mechanisms for postembryonic retinal neurogenesis in mammals. In addition, in the past 10 years there has been an explosion in the use of teleost fish-zebrafish (Danio rerio) in particular-to understand the mechanisms of embryonic retinal neurogenesis in a model vertebrate with genetic resources. This review summarizes the key features of teleost retinal neurogenesis that make it a productive and interesting experimental system, and focuses on the contributions to our knowledge of retinal neurogenesis that uniquely required or significantly benefited from the use of a fish model system.

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The canonical Wnt pathway in early mammalian embryogenesis and stem cell maintenance/differentiation

Cited by 111 publications

References 51 publications

Defined culture conditions of human embryonic stem cells

Defined culture conditions of human embryonic stem cells

Activation of theWnt–βCatenin Pathway in a Cell Population on the Surface of the Forebrain Is Essential for the Establishment of Olfactory Axon Connections

Neurogenesis in the Fish Retina

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