The canine prostate cancer cell line CHP‐1 shows over‐expression of the co‐chaperone small glutamine‐rich tetratricopeptide repeat‐containing protein <i>α</i>

Azakami, Daigo; Nakahira, Rei; Kato, Yuiko; Michishita, Masaki; Kobayashi, Masato; Onozawa, Eri; Bonkobara, Makoto; Takahashi, Koji; Watanabe, Masami; Ishioka, Katsumi; Sako, Toshinori; Ochiai, Kazuhiko; Omi, Toshinori

doi:10.1111/vco.12199

Cited by 11 publications

(23 citation statements)

References 18 publications

(33 reference statements)

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“…Dimerized SGTA is an inhibitor of dynein motor-dependent AR transport and signalling, which is the cause of the androgen-independent condition in human prostate cancer [3, 17]. Canine SGTA also forms dimers and suppresses AR signal transduction [5, 20]. REIC/Dkk-3 interacts with SGTA and interferes with its dimerization recovering AR translocation and signalling [19].…”

Section: Discussionmentioning

confidence: 99%

“…The CHP-1 cell line, which was established from a sample of canine prostate cancer, was positive for cytokeratin AE1/AE3, an epithelial marker [5]. Although prostate cancer CHP-1 cells, which were obtained from a castrated dog, do not express the AR, the cells with AR exhibited androgen-dependent AR signalling (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The canine prostate cancer CHP-1 cell line was established from a prostate mass collected immediately following surgery of a tumour-bearing, 10-year-old, castrated male Jack Russell Terrier breed dog in our university [5]. The 293 T and the CHP-1 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Wako, Osaka, Japan) and PC3 cells were maintained in Ham’s F12 medium (Wako) supplemented with 10% foetal bovine serum (FBS), penicillin (50 IU/mL), and streptomycin (50 μg/mL) under a humidified atmosphere with 5% CO 2 at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, we showed that canine SGTA played an inhibitory role in AR signalling both in canine and human androgen-independent prostate cancer cells [5, 20]. From these findings, we were able to hypothesize that canine SGTA leads to an androgen-independent condition in canine prostate cancer.…”

Section: Introductionmentioning

confidence: 95%

“…Canine prostate cancer shows similar characteristics to human androgen-independent prostate cancer, which progresses without androgen stimulation and is refractory to anti-androgen therapy [4]. To study the similarities between the pathological conditions of both canine and human androgen-independent prostate cancer, we have established a canine androgen-independent prostate cancer cell line, CHP-1 [5]. …”

Section: Introductionmentioning

confidence: 99%

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Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines

et al. 2017

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BackgroundThe pathological condition of canine prostate cancer resembles that of human androgen-independent prostate cancer. Both canine and human androgen receptor (AR) signalling are inhibited by overexpression of the dimerized co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is considered to cause the development of androgen-independency. Reduced expression in immortalised cells (REIC/Dkk-3) interferes with SGTA dimerization and rescues AR signalling. This study aimed to assess the effects of REIC/Dkk-3 and SGTA interactions on AR signalling in the canine androgen-independent prostate cancer cell line CHP-1.ResultsMammalian two-hybrid and Halo-tagged pull-down assays showed that canine REIC/Dkk-3 interacted with SGTA and interfered with SGTA dimerization. Additionally, reporter assays revealed that canine REIC/Dkk-3 restored AR signalling in both human and canine androgen-independent prostate cancer cells. Therefore, we confirmed the interaction between canine SGTA and REIC/Dkk-3, as well as their role in AR signalling.ConclusionsOur results suggest that this interaction might contribute to the development of a novel strategy for androgen-independent prostate cancer treatment. Moreover, we established the canine androgen-independent prostate cancer model as a suitable animal model for the study of this type of treatment-refractory human cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1094-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines

et al. 2017

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Canine prostatic cancer cell line (LuMa) with osteoblastic bone metastasis

Elshafae

Dirksen

Alasonyalilar-Demirer

et al. 2020

The Prostate

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Background: Osteoblastic bone metastasis represents the most common complication in men with prostate cancer (PCa). During progression and bone metastasis, PCa cells acquire properties similar to bone cells in a phenomenon called osteomimicry, which promotes their ability to metastasize, proliferate, and survive in the bone microenvironment. The mechanism of osteomimicry resulting in osteoblastic bone metastasis is unclear. Methods:We developed and characterized a novel canine prostatic cancer cell line (LuMa) that will be useful to investigate the relationship between osteoblastic bone metastasis and osteomimicry in PCa. The LuMa cell line was established from a primary prostate carcinoma of a 13-year old mixed breed castrated male dog. Cell proliferation and gene expression of LuMa were measured and compared to three other canine prostatic cancer cell lines (Probasco, Ace-1, and Leo) in vitro. The effect of LuMa cells on calvaria and murine preosteoblastic (MC3T3-E1) cells was measured by quantitative reverse-transcription polymerase chain reaction and alkaline phosphatase assay. LuMa cells were transduced with luciferase for monitoring in vivo tumor growth and metastasis using different inoculation routes (subcutaneous, intratibial [IT], and intracardiac [IC]). Xenograft tumors and metastases were evaluated using radiography and histopathology. Results: After left ventricular injection, LuMa cells metastasized to bone, brain, and adrenal glands. IT injections induced tumors with intramedullary new bone formation. LuMa cells had the highest messenger RNA levels of osteomimicry genes (RUNX2, RANKL, and Osteopontin [OPN]), CD44, E-cadherin, and MYOF compared to Ace-1, Probasco, and Leo cells. LuMa cells induced growth in calvaria defects and modulated gene expression in MC3T3-E1 cells. Conclusions: LuMa is a novel canine PCa cell line with osteomimicry and stemness properties. LuMa cells induced osteoblastic bone formation in vitro and in vivo.LuMa PCa cells will serve as an excellent model for studying the mechanisms of osteomimicry and osteoblastic bone and brain metastasis in prostate cancer. K E Y W O R D Sbone, canine, dog, metastasis, osteoblast, prostate cancer, tumor

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New In-Depth Analytical Approach of the Porcine Seminal Plasma Proteome Reveals Potential Fertility Biomarkers

et al. 2018

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A complete characterization of the proteome of seminal plasma (SP) is an essential step to understand how SP influences sperm function and fertility after artificial insemination (AI). The purpose of this study was to identify which among characterized proteins in boar SP were differently expressed among AI boars with significantly different fertility outcomes. A total of 872 SP proteins, 390 of them belonging specifically to Sus Scrofa taxonomy, were identified (Experiment 1) by using a novel proteomic approach that combined size exclusion chromatography and solid-phase extraction as prefractionation steps prior to Nano LC-ESI-MS/MS analysis. The SP proteomes of 26 boars showing significant differences in farrowing rate (n = 13) and litter size (n = 13) after the AI of 10 526 sows were further analyzed (Experiment 2). A total of 679 SP proteins were then quantified by the SWATH approach, where the penalized linear regression LASSO revealed differentially expressed SP proteins for farrowing rate (FURIN, AKR1B1, UBA1, PIN1, SPAM1, BLMH, SMPDL3A, KRT17, KRT10, TTC23, and AGT) and litter size (PN-1, THBS1, DSC1, and CAT). This study extended our knowledge of the SP proteome and revealed some SP proteins as potential biomarkers of fertility in AI boars.

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The canine prostate cancer cell line CHP‐1 shows over‐expression of the co‐chaperone small glutamine‐rich tetratricopeptide repeat‐containing protein α

Cited by 11 publications

References 18 publications

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines

Canine prostatic cancer cell line (LuMa) with osteoblastic bone metastasis

New In-Depth Analytical Approach of the Porcine Seminal Plasma Proteome Reveals Potential Fertility Biomarkers

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