The canine isolate Lactobacillus acidophilus LAB20 adheres to intestinal epithelium and attenuates LPS-induced IL-8 secretion of enterocytes in vitro

Kainulainen, Veera; Tang, Yuting; Spillmann, Thomas; Kilpinen, Susanne; Reunanen, Justus; Saris, Per E. J.; Satokari, Reetta

doi:10.1186/s12866-014-0337-9

Cited by 41 publications

(47 citation statements)

References 58 publications

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“…* indicates a significant difference at P < 0.05. and CCL2 when compared with L. sakei. These results are consistent with those of another study in Caco2 cells in which L. acidophilus LB20 were found to adhere to the surface of cells and attenuate LPS mediated inflammatory response by reducing IL-8 production [27]. Another in vitro study reported that L. casei sub sp., rhamnosus could inhibit production of IL-8 and MCP-1 in Caco2 cells stimulated with LPS [23].…”

Section: Figsupporting

confidence: 92%

Functional capabilities of probiotic strains on attenuation of intestinal epithelial cell inflammatory response induced by TLR4 stimuli

Kanmani

Kim

2018

BioFactors

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Intestinal epithelial cells (IECs) respond to intruders and their cellular molecules by displaying inflammatory state that can be abrogated by probiotics. However, the molecular mechanisms underlying the beneficial activity of probiotic strains have yet to be elucidated. This study was conducted to investigate whether probiotic strains have immunoregulatory effects in IECs, and how they respond to bacterial lipopolysaccharide (LPS) in vitro. Caco2 cells were stimulated with LABs and followed by LPS. The expression of different cytokines that involved in toll‐like receptor (TLR) signaling was analyzed. Caco2 cells that were exposed to LPS showed upregulated expression of IL‐6, CXCL8, CCL2, and BPI that were counteracted by LAB strains through the modulation of TLR negative regulators (A20, Tollip, SIGIRR, and IRAKM), p38 MAPK and p65 NF‐κB signaling. Lactobacillus plantarum, L. farciminis, and L. pentosus unveiled better activity as compared to other strains. Moreover, LAB strains were the potent inducers of immunoregulatory cytokines in coculture system. The expression of IL‐10 and TGF‐β were found to be higher as compared with LPS. Conversely, TNF‐α at the protein level was dampened by LAB strains in both the apical and basolateral compartments. Collectively, our results demonstrated that the selected LAB strains exert profound immunoregulatory effects in response to LPS on IECs; however, further studies in vivo and in clinical settings are important to corroborate these effects. © 2018 BioFactors, 45(2):223–235, 2019

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Section: Figsupporting

confidence: 92%

Functional capabilities of probiotic strains on attenuation of intestinal epithelial cell inflammatory response induced by TLR4 stimuli

Kanmani

Kim

2018

BioFactors

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“…EPS of L. delbrueckii have been shown to attenuate ETEC-induced inflammatory responses in porcine IECs, with TLR2/TLR4 playing a central role in the immunomodulatory action [57]. Further, Kainulainen et al [58] showed that EPS of LAB20 might have a role in the immunomodulatory activity of LAB20. Our results indicate that EPS of HDRsEf1 may play a similar role in the immunomodulatory activity of Ef1.…”

Section: Discussionmentioning

confidence: 99%

Enterococcus faeciumHDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes

Tian

Liu

Dai

et al. 2016

Mediators of Inflammation

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The probiotic Enterococcus faecium HDRsEf1 (Ef1) has been shown to have positive effects on piglet diarrhoea, but the mechanism has not yet been elucidated. In this study, using the IPEC-J2 cell line to mimic intestinal epithelial cells and enterotoxigenic Escherichia coli (ETEC) K88ac as a representative intestinal pathogen, the mechanism underlying Ef1 protection against an enteropathogen was investigated. The results demonstrated that Ef1 was effective in displacing K88ac from the IPEC-J2 cell layer. Moreover, Ef1 and its cell-free supernatant (S-Ef1) modulate IL-8 released by IPEC-J2 cells. Ef1 and its cell-free supernatant showed the potential to protect enterocytes from an acute inflammatory response. In addition, Ef1 and its cell-free supernatant increased the transepithelial electrical resistance (TEER) of the enterocyte monolayer, thus strengthening the intestinal barrier against ETEC. These results may contribute to the development of therapeutic interventions using Ef1 in intestinal disorders of piglets.

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“…on transepithelial electrical resistance (TER) of Caco-2 monolayer was determined as previously described (Kainulainen et al, 2015; Reunanen et al, 2015). The Caco-2 cell line is a suitable model for TER assays, because Caco-2 cells undergo enterocytic differentiation as well as express intercellular junctional complexes and, therefore, changes in TER can be measured as a response to external stimuli (Hidalgo et al, 1989; Klingberg et al, 2005; Hsieh et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

et al. 2016

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Sutterella species have been frequently associated with human diseases, such as autism, Down syndrome, and inflammatory bowel disease (IBD), but the impact of these bacteria on health still remains unclear. Especially the interactions of Sutterella spp. with the host are largely unknown, despite of the species being highly prevalent. In this study, we addressed the interaction of three known species of Sutterella with the intestinal epithelium and examined their adhesion properties, the effect on intestinal barrier function and the pro-inflammatory capacity in vitro. We also studied the relative abundance and prevalence of the genus Sutterella and Sutterella wadsworthensis in intestinal biopsies of healthy individuals and patients with celiac disease (CeD) or IBD. Our results show that Sutterella spp. are abundant in the duodenum of healthy adults with a decreasing gradient toward the colon. No difference was detected in the prevalence of Sutterella between the pediatric IBD or CeD patients and the healthy controls. Sutterella parvirubra adhered better than the two other Sutterella spp. to differentiated Caco-2 cells and was capable of decreasing the adherence of S. wadsworthensis, which preferably bound to mucus and human extracellular matrix proteins. Furthermore, only S. wadsworthensis induced an interleukin-8 production in enterocytes, which could be due to different lipopolysaccharide structures between the species. However, its pro-inflammatory activity was modest as compared to non-pathogenic Escherichia coli. Sutterella spp. had no effect on the enterocyte monolayer integrity in vitro. Our findings indicate that the members of genus Sutterella are widely prevalent commensals with mild pro-inflammatory capacity in the human gastrointestinal tract and do not contribute significantly to the disrupted epithelial homeostasis associated with microbiota dysbiosis and increase of Proteobacteria. The ability of Sutterella spp. to adhere to intestinal epithelial cells indicate that they may have an immunomodulatory role.

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The canine isolate Lactobacillus acidophilus LAB20 adheres to intestinal epithelium and attenuates LPS-induced IL-8 secretion of enterocytes in vitro

Cited by 41 publications

References 58 publications

Functional capabilities of probiotic strains on attenuation of intestinal epithelial cell inflammatory response induced by TLR4 stimuli

Functional capabilities of probiotic strains on attenuation of intestinal epithelial cell inflammatory response induced by TLR4 stimuli

Enterococcus faeciumHDRsEf1 Protects the Intestinal Epithelium and Attenuates ETEC-Induced IL-8 Secretion in Enterocytes

Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

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