The Cancer Genome Atlas (TCGA) based m
            <sup>6</sup>
            A methylation-related genes predict prognosis in hepatocellular carcinoma

Li, Jun; Sun, Guili; Pan, Shang‐Ling; Qin, Mengbin; Ouyang, Rong; Li, Zhongzhuan; Huang, Jiean

doi:10.1080/21655979.2020.1787764

Cited by 72 publications

(59 citation statements)

References 40 publications

(53 reference statements)

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“…In this study, the FRLS contained 15 ferroptosis-related lncRNAs, several of which have been confirmed to be correlated with tumor progression. For example, SNAI3-AS1 promotes PEG10-mediated proliferation and metastasis by acting as a sponge for miR-27-3p and miR-34a-5p in hepatocellular carcinoma ( Liu J. et al, 2020 ). WDFY3-AS2 suppresses cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition (EMT) via miR-18a/RORA axis in ovarian cancer ( Zhou et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

Zheng

Zhou

Qiu

et al. 2021

Front. Cell Dev. Biol.

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Recent studies have demonstrated that long non-coding RNAs (lncRNAs) are implicated in the regulation of tumor cell ferroptosis. However, the prognostic value of ferroptosis-related lncRNAs has never been comprehensively explored in glioma. In this study, the transcriptomic data and clinical information of glioma patients were downloaded from TCGA, CGGA and Rembrandt databases. We identified 24 prognostic ferroptosis-related lncRNAs, 15 of which (SNAI3-AS1, GDNF-AS1, WDFY3-AS2, CPB2-AS1, WAC-AS1, SLC25A21-AS1, ARHGEF26-AS1, LINC00641, LINC00844, MIR155HG, MIR22HG, PVT1, SNHG18, PAXIP1-AS2, and SBF2-AS1) were used to construct a ferroptosis-related lncRNAs signature (FRLS) according to the least absolute shrinkage and selection operator (LASSO) regression. The validity of this FRLS was verified in training (TCGA) and validation (CGGA and Rembrandt) cohorts, respectively. The Kaplan-Meier curves revealed a significant distinction of overall survival (OS) between the high- and low-risk groups. The receiver operating characteristic (ROC) curves exhibited robust prognostic capacity of this FRLS. A nomogram with improved accuracy for predicting OS was established based on independent prognostic factors (FRLS, age, and WHO grade). Besides, patients in the high-risk group had higher immune, stroma, and ESTIMATE scores, lower tumor purity, higher infiltration of immunosuppressive cells, and higher expression of immune checkpoints. Patients in the low-risk group benefited significantly from radiotherapy, while no survival benefit of radiotherapy was observed for those in the high-risk group. In conclusion, we identified the prognostic ferroptosis-related lncRNAs in glioma, and constructed a prognostic signature which was associated with the immune landscape of glioma microenvironment and radiotherapy response.

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Section: Discussionmentioning

confidence: 99%

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

Zheng

Zhou

Qiu

et al. 2021

Front. Cell Dev. Biol.

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“…Meanwhile, it was also noted that m6A was distributed differently in mRNA and lncRNA, indicating that m6A modification of lncRNA may be different from mRNA, and its specific mechanism needs to be further studied. 31 METTL3 has been indicated to be upregulated in HCC 32 . while METTL3 and METTL14 hold opposite regulatory roles in HCC.…”

Section: Discussionmentioning

confidence: 99%

m6A-Induced LncRNA MEG3 Suppresses the Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cell Through miR-544b/BTG2 Signaling

Wu¹,

Pang²,

Li³

et al. 2021

OTT

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Objective Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Long non-coding RNA plays an important role in the development of HCC. This study analyzed the impact of MEG3 on malignant behavior of HCC and explored its possible molecular mechanism. Methods Expression of MEG3 in HCC tissues and cell lines was measured by qRT-PCR. Transfection efficiency of MEG3 was verified by qRT-PCR. Cell proliferation, transwell migration, invasion and cell cloning assays were used to detect the effect of MEG3 on the proliferation, migration and invasion ability of HCC cells. The bioinformatics analysis was applied to predict the binding between miR-544b and MEG3 as well as BTG2. Luciferase reporter assay was performed to verify their interaction. Finally, the m6A modification of MEG3 by METTL3 was identified through RIP experiments. Results MEG3 was lowly expressed in HCC tissues and cells. Overexpression of MEG3 inhibits the proliferation, migration and invasion of HCC cells. MiR-544b can be sponged by MEG3, and overexpression of miR-544b reverses the anti-cancer effect of MEG3. We further confirmed that BTG2 gene is the target gene of miR-544b. Epigenetic studies have shown that METTL3-mediated N 6 -methyladenosine modification led to MEG3 downregulation. Conclusion In HCC, MEG3 and BTG2 are lowly expressed while miR-544b is highly expressed. MEG3 regulates the expression of BTG2 through miR-544b, thus affecting the malignant behavior of HCC. METTL3 regulates the m6A modification of MEG3 and its expression. This study clarified the role of MEG3/miR-544b/BTG2 axis in HCC and also provided new targets for HCC research.

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“…YTHDF2 expression is closely associated with HCC malignance, and can be inhibited by miR-145 [152]. YTHDF2, YTHDF1, METTL3, KIAA1429, and ZC3H13 could be a prognostic signature of malignant HCC patients [52,[157][158][159]. The expression of two genes (hnRNPA2B1 and RBM15) are closely related to the TNM stage and metastasis risk and can act as a prognostic indicator for HBV-related HCC patients in the early TNM stage [160].…”

Section: Prognosis and Therapeutic Potency Of Targeting Altered M 6 Amentioning

confidence: 99%

The emerging roles of m⁶A modification in liver carcinogenesis

Pan¹,

Li²

2021

Int. J. Biol. Sci.

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The 'epitranscriptome', a collective term for chemical modifications that influence the structure, metabolism, and functions of RNA, has recently emerged as vitally important for the regulation of gene expression. N 6 -methyladenosine (m 6 A), the most prevalent mammalian mRNA internal modification, has been demonstrated to have a pivotal role in almost all vital bioprocesses, such as stem cell self-renewal and differentiation, heat shock or DNA damage response, tissue development, and maternal-to-zygotic transition. Hepatocellular carcinoma (HCC) is prevalent worldwide with high morbidity and mortality because of late diagnosis at an advanced stage and lack of effective treatment strategies. Epigenetic modifications including DNA methylation and histone modification have been demonstrated to be crucial for liver carcinogenesis. However, the role and underlying molecular mechanism of m 6 A in liver carcinogenesis are mostly unknown. In this review, we summarize recent advances in the m 6 A region and how these new findings remodel our understanding of m 6 A regulation of gene expression. We also describe the influence of m 6 A modification on liver carcinoma and lipid metabolism to instigate further investigations of the role of m 6 A in liver biological diseases and its potential application in the development of therapeutic strategies.

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The Cancer Genome Atlas (TCGA) based m ⁶ A methylation-related genes predict prognosis in hepatocellular carcinoma

Cited by 72 publications

References 40 publications

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

m6A-Induced LncRNA MEG3 Suppresses the Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cell Through miR-544b/BTG2 Signaling

The emerging roles of m⁶A modification in liver carcinogenesis

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The Cancer Genome Atlas (TCGA) based m 6 A methylation-related genes predict prognosis in hepatocellular carcinoma

Cited by 72 publications

References 40 publications

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

m6A-Induced LncRNA MEG3 Suppresses the Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cell Through miR-544b/BTG2 Signaling

The emerging roles of m6A modification in liver carcinogenesis

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The Cancer Genome Atlas (TCGA) based m ⁶ A methylation-related genes predict prognosis in hepatocellular carcinoma

The emerging roles of m⁶A modification in liver carcinogenesis