The Cancer Chemopreventive Agent Resveratrol Is Incorporated into Model Membranes and Inhibits Protein Kinase C α Activity

Garcı́a-Garcı́a, Josefa; Micol, Vicente; Godos, Ana de; Gómez‐Fernández, Juan C.

doi:10.1006/abbi.1999.1507

Cited by 68 publications

(34 citation statements)

References 37 publications

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“…[29][30][31][32] The mechanisms of resveratrol-induced suppression of cellular proliferation have been reported to involve the induction of apoptosis through Fas/CD95, mitochondrial and p53 mediated pathways, [29][30][31][32] upregulation of CHOP gene expression. 33 In addition, resveratrol suppressed NF-kB and protein kinase-C activity, and modulated the angiogenesis and expression of various growth factors as well as NO/ NOS. [34][35][36][37][38] Our group previously reported that resveratrol triggers apoptosis through up-regulating longevity assurance (LASS) genes that generate apoptotic ceramides and by down-regulating sphingosine kinase-1 and glucosylceramide synthase genes that convert apoptotic ceramides to antiapoptotic sphingosine-1-phosphate and glucosylceramide lipids, respectively, in acute myeloid leukemia and CML cells.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

2013

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Chronic lymphocytic leukemia (CLL), defined by accumulation of pathogenic B cells, has a very complex biology due to various factors such as inherited, host, and enviromental factors. Recently, finding new therapeutic agents or development of novel treatment strategies have been paid attention. Resveratrol and quercetin, important phytoalexins found in many plants, have been reported to have cytotoxic effects on various types of cancer. In this study, we examined cytotoxic, cytostatic, and apoptotic effects of these two important phenolic compounds on 232B4 human CLL cells. Cytotoxic effects of resveratrol and quercetin were determined by MTT cell proliferation assay. Changes in caspase-3 enzyme activity were measured using caspase-3 colorimetric assay. Annexin V-FITC/PI double staining was performed to measure apoptotic cell population. Effects of resveratrol and quercetin on cell cycle profiles of CLL cells were investigated by flow cytometry. Treatment of CLL cells with resveratrol and quercetin caused dose dependent inhibition of cell proliferation and increased apoptotic cell population through induction of caspase-3 activity. Cell cycle analysis displayed cell cycle arrest mainly in G0/G1 for both polyphenols. Our data, in total, showed for the first time that resveratrol and quercetin might block CLL growth through inducing apoptosis and cell cycle arrest.

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Section: Discussionmentioning

confidence: 99%

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

2013

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“…5). Indeed, resveratrol is able to act on the preceding stages by inhibiting the phosphorylation and the activity of PKC [59][60][61][62]. Resveratrol inhibits the PKCcatalyzed phosphorylation of arginin-rich protein substrate in a non competitive manner [63].…”

Section: A) Resveratrol Chemoprevention By Inhibition Of Kinase Cascadementioning

confidence: 99%

Resveratrol as a Chemopreventive Agent: A Promising Molecule for Fighting Cancer

Delmas¹,

Lançon²,

Colin³

et al. 2006

CDT

352

223

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“…However, the co-treatment with bFGF plus tresveratrol (43.8 μM/ disk) abolished the stimulant effect afforded by stilbene alone. Thus we hypothesize, once evidences show that bFGF partially mediates the mitogenic activity through PKC activation (49), this growth factor could be furnishing an additional target to resveratrol, in this way decreasing its availability.…”

Section: Discussionmentioning

confidence: 98%

Trans-Resveratrol Inhibits Early Blood Vessel Formation (Vasculogenesis) Without Impairment of Embryonic Growth

et al. 2008

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Abstract. Resveratrol is a stilbene compound found in grapes and other sources. In this study we examined the effects of trans-resveratrol (4.38 -438 μM/ implant) in the vasculogenesis of yolk-sac membranes and its capacity to improve chick embryo growth. High concentrations of the stilbene (43.8 -438 μM) significantly inhibited early vessel formation, decreasing the percentage vitelline vessels of 3.5-day embryos by 50% compared to the control. In addition, basic fibroblast growth factor-stimulated vasculogenesis (140% of vessels as compared to control) was partially reversed by t-resveratrol (35% of inhibition) and treatments with cyclooxygenase inhibitors (acetylsalicylic acid and indomethacin) as well a protein-kinase C (PKC) activator (phorbol-12,13-dibutyrate) decreased the vessel number to 60%, 50%, and 44%, respectively. Treatments with t-resveratrol (4.38 -43.8 μM/ implant) significantly increased the body length of embryos incubated in vitro uncoupled from any impairment in the body shape or detectable embryotoxic effect. We suggest that the antivasculogenic activity and the enhancement in embryonic growth promoted by non acute treatments with t-resveratrol were, at least in part, due to PKC inhibition. We suggest that t-resveratrol can be usable not only as a reliable functional nutriment, but also is useful for the development of prophylactic and / or therapeutic agents for treatment of angiogenic-degenerative diseases.

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The Cancer Chemopreventive Agent Resveratrol Is Incorporated into Model Membranes and Inhibits Protein Kinase C α Activity

Cited by 68 publications

References 37 publications

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

Resveratrol as a Chemopreventive Agent: A Promising Molecule for Fighting Cancer

Trans-Resveratrol Inhibits Early Blood Vessel Formation (Vasculogenesis) Without Impairment of Embryonic Growth

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